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Literature summary for 3.4.24.B6 extracted from

  • Llano, E.; Pendas, A.M.; Knauper, V.; Sorsa, T.; Salo, T.; Salido, E.; Murphy, G.; Simmer, J.P.; Bartlett, J.D.; Lopez-Otin, C.
    Identification and structural and functional characterization of human enamelysin (MMP-20) (1997), Biochemistry, 36, 15101-15108.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, chromosome mapping to 11q22, expression in Escherichia coli BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
tissue matrix metalloprotease inhibitor TIMP-2 complete inhibition of amylogenin cleavage Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ enzyme contains a zinc-binding site in the catalytic domain with the consensus sequence HEXGHXXGXXH at positions 226-236 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
recombinant enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amylogenin + H2O Homo sapiens involved in tooth formation ?
-
?
proteins + H2O Homo sapiens
-
peptides
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60882
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification enzyme proform contains a prodomain motif PRCGVPD, the proform is in a latent state Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
proteolytic degradation of ameloblastin active site with the consensus sequence HEXGHXXGXXH at positions 226-236 Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
recombinant enzyme from inclusion bodies expressed in Escherichia coli Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
odontoblast
-
Homo sapiens
-
tooth
-
Homo sapiens
-
tooth enamel
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylogenin + H2O involved in tooth formation Homo sapiens ?
-
?
amylogenin + H2O
-
Homo sapiens 4 major peptide fragments of MWs 24 kDa, 23 kDa, 22 kDa, and 20 kDa
-
?
Mca-PLA-norvaline-Dpa-AR-NH2 + H2O synthetic fluorogenic substrate Homo sapiens ?
-
?
Mca-PLGL-Dpa-AR-NH2 + H2O synthetic fluorogenic substrate Homo sapiens ?
-
?
proteins + H2O
-
Homo sapiens peptides
-
?

Synonyms

Synonyms Comment Organism
enamelysin
-
Homo sapiens
M10.019 Merops-ID Homo sapiens
MMP-20
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens