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3.4.24.B6: matrix metalloproteinase-20

This is an abbreviated version!
For detailed information about matrix metalloproteinase-20, go to the full flat file.

Word Map on EC 3.4.24.B6

Reaction

proteolytic cleavage of ameloblastin =

Synonyms

enamel metalloproteinase, enamel protease, enamelysin, M10.019, matrix metalloproteinase 20, MMP-20, MMP20

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.B6 matrix metalloproteinase-20

Purification

Purification on EC 3.4.24.B6 - matrix metalloproteinase-20

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
anion exchange chromatography and gel filtration
-
His-TRAP chelating nickel affinity column chromatography
-
native enzyme from soft enamel-alkaline extract by anion exchange chromatography, ultrafiltration, and heparin affinity chromatography
-
Ni-NTA column chromatography
-
nickel resin chromatography
purified on Ni column
-
Q-Sepharose column chromatography and heparin Sepharose 6 column chromatography
recombinant from Escherichia coli
recombinant His-tagged MMP-20 by metal affinity chromatography
-
recombinant His-tagged pro-enzyme from Escherichia coli
recombinant wild-type and inactive mutant E227A