Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.22.35: Histolysain

This is an abbreviated version!
For detailed information about Histolysain, go to the full flat file.

Word Map on EC 3.4.22.35

Reaction

hydrolysis of proteins, including basement membrane collagen and azocasein. Preferential cleavage: Arg-Arg-/- in small molecule substrates including Z-Arg-Arg-/-NHMec =

Synonyms

Amebapain, amoebapain, CP1, CP2, CP5, CP6, cysteine protease, cysteine protease 1, cysteine protease 2, cysteine protease 5, cysteine protease 6, cysteine proteinase 1, cysteine proteinase 5, EhCP1, EhCP112, EhCP2, EhCP5, Entamoeba histolytica 112kDa adhesin, Entamoeba histolytica cysteine protease, Entamoeba histolytica cysteine proteinase, Entamoeba histolytica cysteine proteinase 5, Entamoeba histolytica cysteine-proteinase, Entamoeba histolytica neutral thiol proteinase, Histolysin, More, PCP5, ppEhCP-B9, Proteinase, Entamoeba histolytica cysteine

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.35 Histolysain

Inhibitors

Inhibitors on EC 3.4.22.35 - Histolysain

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-hydroxymercuribenzoate
-
E64 inhibits cell growth and is cytopathic/lethal for E64-sensitive and E64-resistant amoebae
4-[(2S,3S)-carboxyoxiran-2-ylcarbonyl-L-leucylamido]butylguanidine
-
i.e. trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane or E64
chicken cystatin
-
-
-
chymostatin
-
-
cystatin
-
-
-
cysteine protease inhibitor 1
-
-
-
cysteine protease inhibitor 2
-
inhibits isoform CP5 approximately 20times more efficient than isoform CP2
-
EDTA
-
slight inhibition at 10 mM
EhICP1
Entamoeba histolytica inhibitor for cysteine protease type 1; Entamoeba histolytica inhibitor for cysteine protease type 1; Entamoeba histolytica inhibitor for cysteine protease type 1, inhibition also by regulation of CP5 processing
-
EhICP2
Entamoeba histolytica inhibitor for cysteine protease type 2; Entamoeba histolytica inhibitor for cysteine protease type 2; Entamoeba histolytica inhibitor for cysteine protease type 2, inhibition also by regulation of CP5 processing
-
iodoacetamide
iodoacetic acid
-
-
L-3-carboxy-2,3-trans-epoxypropionyl-leucyl-amido(4-guanidino)butane
-
i.e. E-64
L-trans-epoxysuccinyl-(ethoxy)-Leu-3-methylbutylamide ethyl ester
-
i.e. E64d, inhibits cell growth and is cytopathic/lethal for E64-sensitive and E64-resistant amoebae
L-trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
-
L-trans-epoxysuccinyl-Leu-4-guanidinobutylamide
-
i.e. E64, inhibits cell growth in E64-resistant by about 80% and is cytopathic/lethal for E64-sensitive amoebae
N-ethylmaleimide
-
-
N-methylpiperazine-urea-phenylalanylhomoarginylvinylsulfone-benzene
-
WRR483, inhibitor addition to medium fully prevents invasion of parasite
N-methylpiperazine-urea-phenylalanylhomophenylalanylvinylsulfone-benzene
-
K11777, inhibitor addition to medium partially prevents invasion of parasite
Nalpha-4-tosyl-L-lysine chloromethylketone
-
-
Natural and synthetic inhibitors of cysteine proteinases
-
-
-
p-hydroxymercuribenzoate
-
Peptidyldiazomethanes
-
-
Phenanthroline
-
slight inhibition at 10 mM
tosylsulfonylleucylchloromethyl ketone
-
i.e. TLCK
tosylsulfonylphenylalanylchloromethyl ketone
-
i.e. TPCK
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
Z-Phe-Ala-diazomethylketone
-
E64 inhibits cell growth and is cytopathic/lethal for E64-sensitive and E64-resistant amoebae
Z-Phe-Phe-diazomethylketone
-
25% inhibition of E64-sensitive and E64-resistant amoba growth
Zn2+
-
77% inhibition of proteolytic activity at 2.5 mM, completely reversible by L-cystein, phenanthroline, and L-histidine, Zn2+ interferes with the fibronectin binding of the amoeba
additional information
-