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Literature summary for 3.4.22.35 extracted from

  • Melendez-Lopez, S.G.; Herdman, S.; Hirata, K.; Choi, M.H.; Choe, Y.; Craik, C.; Caffrey, C.R.; Hansell, E.; Chavez-Munguia, B.; Chen, Y.T.; Roush, W.R.; McKerrow, J.; Eckmann, L.; Guo, J.; Stanley, S.L.; Reed, S.L.
    Use of recombinant Entamoeba histolytica cysteine proteinase 1 to identify a potent inhibitor of amebic invasion in a human colonic model (2007), Eukaryot. Cell, 6, 1130-1136.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
cysteine 5-10 mM activate refolded protein Entamoeba histolytica
dithiothreitol 2-5 mM activate refolded protein in a Tris, phosphate or citrate buffer containing EDTA, pH 7.0-7.5 Entamoeba histolytica

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag and thioredoxin fusin protein in Escherichia coli Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
N-methylpiperazine-urea-phenylalanylhomoarginylvinylsulfone-benzene WRR483, inhibitor addition to medium fully prevents invasion of parasite Entamoeba histolytica
N-methylpiperazine-urea-phenylalanylhomophenylalanylvinylsulfone-benzene K11777, inhibitor addition to medium partially prevents invasion of parasite Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic vesicle large cytoplasmic vesicles Entamoeba histolytica 31410
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 31000, SDS-PAGE, refolded, active protein Entamoeba histolytica
50000
-
x * 50000, SDS-PAGE, predicted molecular mass for thio-pro-EhCP1 of 50300 Da (36300 Da for the zymogen form of EhCP1, 13000 Da for the thioredoxin peptide, and 9000 Da for the six-His tag) Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Entamoeba histolytica cysteine proteinases play a central role in tissue invasion and disruption of host defenses by digesting components of the extracellular matrix, immunoglobulins, complement, and cytokines ?
-
?
additional information Entamoeba histolytica HM1:IMSS cysteine proteinases play a central role in tissue invasion and disruption of host defenses by digesting components of the extracellular matrix, immunoglobulins, complement, and cytokines ?
-
?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-
Entamoeba histolytica HM1:IMSS
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag under denaturating conditions Entamoeba histolytica

Renatured (Commentary)

Renatured (Comment) Organism
-
Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
C3 protein + H2O protein from human Entamoeba histolytica ?
-
?
immunoglobulin G + H2O heavy chain, protein from human Entamoeba histolytica ?
-
?
immunoglobulin G + H2O heavy chain, protein from human Entamoeba histolytica HM1:IMSS ?
-
?
additional information cysteine proteinases play a central role in tissue invasion and disruption of host defenses by digesting components of the extracellular matrix, immunoglobulins, complement, and cytokines Entamoeba histolytica ?
-
?
additional information no activity with Z-Phe-Arg-4-amido-7-methylcoumarin Entamoeba histolytica ?
-
?
additional information cysteine proteinases play a central role in tissue invasion and disruption of host defenses by digesting components of the extracellular matrix, immunoglobulins, complement, and cytokines Entamoeba histolytica HM1:IMSS ?
-
?
additional information no activity with Z-Phe-Arg-4-amido-7-methylcoumarin Entamoeba histolytica HM1:IMSS ?
-
?
pro-interleukin-18 + H2O protein from human Entamoeba histolytica ?
-
?
Z-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Entamoeba histolytica Z-Ala-Arg-Arg + 7-amino-4-methylcoumarin
-
?
Z-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Entamoeba histolytica HM1:IMSS Z-Ala-Arg-Arg + 7-amino-4-methylcoumarin
-
?
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Entamoeba histolytica Z-Arg-Arg + 7-amino-4-methylcoumarin
-
?
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Entamoeba histolytica HM1:IMSS Z-Arg-Arg + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE, refolded, active protein Entamoeba histolytica
? x * 50000, SDS-PAGE, predicted molecular mass for thio-pro-EhCP1 of 50300 Da (36300 Da for the zymogen form of EhCP1, 13000 Da for the thioredoxin peptide, and 9000 Da for the six-His tag) Entamoeba histolytica

Synonyms

Synonyms Comment Organism
cysteine proteinase 1
-
Entamoeba histolytica
EhCP1
-
Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
almost fully active between pH 5.5 and 7.5 Entamoeba histolytica

pH Range

pH Minimum pH Maximum Comment Organism
5 8 50% of activity at pH 5 and pH 8 Entamoeba histolytica