Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | 5-10 mM activate refolded protein | Entamoeba histolytica | |
dithiothreitol | 2-5 mM activate refolded protein in a Tris, phosphate or citrate buffer containing EDTA, pH 7.0-7.5 | Entamoeba histolytica |
Cloned (Comment) | Organism |
---|---|
expressed as His-tag and thioredoxin fusin protein in Escherichia coli | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-methylpiperazine-urea-phenylalanylhomoarginylvinylsulfone-benzene | WRR483, inhibitor addition to medium fully prevents invasion of parasite | Entamoeba histolytica | |
N-methylpiperazine-urea-phenylalanylhomophenylalanylvinylsulfone-benzene | K11777, inhibitor addition to medium partially prevents invasion of parasite | Entamoeba histolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic vesicle | large cytoplasmic vesicles | Entamoeba histolytica | 31410 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE, refolded, active protein | Entamoeba histolytica |
50000 | - |
x * 50000, SDS-PAGE, predicted molecular mass for thio-pro-EhCP1 of 50300 Da (36300 Da for the zymogen form of EhCP1, 13000 Da for the thioredoxin peptide, and 9000 Da for the six-His tag) | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Entamoeba histolytica | cysteine proteinases play a central role in tissue invasion and disruption of host defenses by digesting components of the extracellular matrix, immunoglobulins, complement, and cytokines | ? | - |
? | |
additional information | Entamoeba histolytica HM1:IMSS | cysteine proteinases play a central role in tissue invasion and disruption of host defenses by digesting components of the extracellular matrix, immunoglobulins, complement, and cytokines | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Entamoeba histolytica HM1:IMSS | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag under denaturating conditions | Entamoeba histolytica |
Renatured (Comment) | Organism |
---|---|
- |
Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
C3 protein + H2O | protein from human | Entamoeba histolytica | ? | - |
? | |
immunoglobulin G + H2O | heavy chain, protein from human | Entamoeba histolytica | ? | - |
? | |
immunoglobulin G + H2O | heavy chain, protein from human | Entamoeba histolytica HM1:IMSS | ? | - |
? | |
additional information | cysteine proteinases play a central role in tissue invasion and disruption of host defenses by digesting components of the extracellular matrix, immunoglobulins, complement, and cytokines | Entamoeba histolytica | ? | - |
? | |
additional information | no activity with Z-Phe-Arg-4-amido-7-methylcoumarin | Entamoeba histolytica | ? | - |
? | |
additional information | cysteine proteinases play a central role in tissue invasion and disruption of host defenses by digesting components of the extracellular matrix, immunoglobulins, complement, and cytokines | Entamoeba histolytica HM1:IMSS | ? | - |
? | |
additional information | no activity with Z-Phe-Arg-4-amido-7-methylcoumarin | Entamoeba histolytica HM1:IMSS | ? | - |
? | |
pro-interleukin-18 + H2O | protein from human | Entamoeba histolytica | ? | - |
? | |
Z-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Entamoeba histolytica | Z-Ala-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
Z-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Entamoeba histolytica HM1:IMSS | Z-Ala-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Entamoeba histolytica | Z-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Entamoeba histolytica HM1:IMSS | Z-Arg-Arg + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE, refolded, active protein | Entamoeba histolytica |
? | x * 50000, SDS-PAGE, predicted molecular mass for thio-pro-EhCP1 of 50300 Da (36300 Da for the zymogen form of EhCP1, 13000 Da for the thioredoxin peptide, and 9000 Da for the six-His tag) | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
cysteine proteinase 1 | - |
Entamoeba histolytica |
EhCP1 | - |
Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
almost fully active between pH 5.5 and 7.5 | Entamoeba histolytica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 50% of activity at pH 5 and pH 8 | Entamoeba histolytica |