3.4.22.35: Histolysain

This is an abbreviated version!
For detailed information about Histolysain, go to the full flat file.

Reaction

hydrolysis of proteins, including basement membrane collagen and azocasein. Preferential cleavage: Arg-Arg-/- in small molecule substrates including Z-Arg-Arg-/-NHMec =

Synonyms

Amebapain, amoebapain, CP1, CP2, CP5, CP6, cysteine protease, cysteine protease 1, cysteine protease 2, cysteine protease 5, cysteine protease 6, cysteine proteinase 1, cysteine proteinase 5, EhCP1, EhCP112, EhCP2, EhCP5, Entamoeba histolytica 112kDa adhesin, Entamoeba histolytica cysteine protease, Entamoeba histolytica cysteine proteinase, Entamoeba histolytica cysteine proteinase 5, Entamoeba histolytica cysteine-proteinase, Entamoeba histolytica neutral thiol proteinase, Histolysin, More, PCP5, ppEhCP-B9, Proteinase, Entamoeba histolytica cysteine

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.35 Histolysain

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Results	in table
7	Activating Compound
8	AA Sequence
1	CAS Registry Number
12	Cloned(Commentary)
5	Expression
4	General Information
1	General Stability
44	Inhibitors
12	Ki Value [mM]
2	KM Value [mM]
17	Localization
15	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
78	Organism
13	pH Optimum
5	pH Range
1	pH Stability
3	pI Value
3	Posttranslational Modification
1	Protein Variants
16	Purification (Commentary)
3	Reaction
8	Reaction Type
41	Reference
6	Renatured (Commentary)
19	Source Tissue
8	Specific Activity [micromol/min/mg]
109	Substrates and Products (Substrate)
21	Subunits
67	Synonyms
6	Temperature Optimum [°C]
2	Temperature Range [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Cloned

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Cloned on EC 3.4.22.35 - Histolysain

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

DNA and amino acid sequence determination

Entamoeba histolytica

Q95030

653256

expressed as His-tag and thioredoxin fusin protein in Escherichia coli

Entamoeba histolytica

-

683417

expressed as His-tag fusion protein in Escherichia coli BL21(DE3), formation of inclusion bodies

Entamoeba histolytica

Q01957, Q01958

683468

expressed in Escherichia coli BL21(DE3) cells

Entamoeba histolytica

C4LSI6

732864

expressed in Escherichia coli C43 cells

Entamoeba histolytica

-

731356

expression of partial gene ehcp1 in Escherichia coli as GST-fusion protein, expression of functional EhCP2 in insect cells using the baculovirus transfection system

Entamoeba histolytica

-

668769

expression of the His-tagged inactive pro-enzyme in Escherichia coli BL21(DE3)

Entamoeba histolytica

-

653742

functional expression of EhCP5

Entamoeba histolytica

-

668713

gene ehcp112, functional expression of the His-tagged full-length EhCP112 zymogen, containing the signal peptide and the pro-peptide, in Escherichia coli strain BL21

Entamoeba histolytica

-

668210

gene Ehcp112, gene is encoded adjacent to Ehadh112, the latter encoding an adhesin that is covalently connected to the enzyme by electrostatic forces, DNA sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli BL21(DE3)

Entamoeba histolytica

-

653261

genes ehcp1, ehcp2, and ehcp5, stable episomal transfection, overexpression of EhCP5 highly increases activity of EhCP1, EhCP2, and EhCP5 in recombinant amoeba and cytopathic activity, while overexpression of EhCP1 or 2 enhances only the single activity and moderately enhances cytopathic activity, overview

Entamoeba histolytica

-

670192

recombinant expression of the His-tagged enzyme in Escherichia coli inclusion bodies

Entamoeba histolytica

-

701062