Cloned (Comment) | Organism |
---|---|
expression of partial gene ehcp1 in Escherichia coli as GST-fusion protein, expression of functional EhCP2 in insect cells using the baculovirus transfection system | Entamoeba histolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | the enzyme is secreted and localized on the cell surface | Entamoeba histolytica | 9986 | - |
lysosome | lysosome-like vesicles | Entamoeba histolytica | 5764 | - |
membrane | the enzyme contains a transmembrane segment | Entamoeba histolytica | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
1 * 27000, about | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Entamoeba histolytica | the enzyme degrades a number of proteins, including those of the extracellular matrix | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba dispar | - |
gene edcp2 or acp2 | - |
Entamoeba histolytica | - |
gene ehcp2 or acp2 encodes histolysain, gene ehcp1 or acp1 encodes amoebapain | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | no N-glycosylation | Entamoeba histolytica |
Purification (Comment) | Organism |
---|---|
to homogeneity | Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the enzyme is expressed and active in different life cycle stages | Entamoeba histolytica | - |
trophozoite | - |
Entamoeba histolytica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Gly-Phe-Phe + H2O | substrate of amoebapain | Entamoeba histolytica | Arg-Gly + Phe-Phe | - |
? | |
azocasein + H2O | - |
Entamoeba histolytica | ? | - |
? | |
Azocoll + H2O | - |
Entamoeba histolytica | ? | - |
? | |
additional information | the enzyme degrades a number of proteins, including those of the extracellular matrix | Entamoeba histolytica | ? | - |
? | |
additional information | the enzyme degrades a number of proteins, including those of the extracellular matrix, but also acts on small molecule substrates showing endopeptidase and exopeptidase-like activities, amoebapain hydrolyzes unblocked tetrapeptides with basic residues at P2 | Entamoeba histolytica | ? | - |
? | |
Z-Arg-Arg-4-methoxy-2-nitroanilide + H2O | - |
Entamoeba histolytica | Z-Arg-Arg + 4-methoxy-2-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27000, about | Entamoeba histolytica |
More | the enzyme contains an RGD integrin attachment motif, structure-function overview | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
amoebapain | - |
Entamoeba histolytica |
EhCP1 | - |
Entamoeba histolytica |
EhCP2 | - |
Entamoeba histolytica |
Histolysin | - |
Entamoeba histolytica |
Histolysin | - |
Entamoeba dispar |
More | the enzyme belongs to the C1 peptidase family | Entamoeba histolytica |
More | the enzyme belongs to the C1 peptidase family | Entamoeba dispar |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | dependent on the substrate | Entamoeba histolytica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Entamoeba histolytica | - |
- |
additional information |