3.4.22.35: Histolysain

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For detailed information about Histolysain, go to the full flat file.

Reaction

hydrolysis of proteins, including basement membrane collagen and azocasein. Preferential cleavage: Arg-Arg-/- in small molecule substrates including Z-Arg-Arg-/-NHMec =

Synonyms

Amebapain, amoebapain, CP1, CP2, CP5, CP6, cysteine protease, cysteine protease 1, cysteine protease 2, cysteine protease 5, cysteine protease 6, cysteine proteinase 1, cysteine proteinase 5, EhCP1, EhCP112, EhCP2, EhCP5, Entamoeba histolytica 112kDa adhesin, Entamoeba histolytica cysteine protease, Entamoeba histolytica cysteine proteinase, Entamoeba histolytica cysteine proteinase 5, Entamoeba histolytica cysteine-proteinase, Entamoeba histolytica neutral thiol proteinase, Histolysin, More, PCP5, ppEhCP-B9, Proteinase, Entamoeba histolytica cysteine

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.35 Histolysain

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Results	in table
7	Activating Compound
8	AA Sequence
1	CAS Registry Number
12	Cloned(Commentary)
5	Expression
4	General Information
1	General Stability
44	Inhibitors
12	Ki Value [mM]
2	KM Value [mM]
17	Localization
15	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
78	Organism
13	pH Optimum
5	pH Range
1	pH Stability
3	pI Value
3	Posttranslational Modification
1	Protein Variants
16	Purification (Commentary)
3	Reaction
8	Reaction Type
41	Reference
6	Renatured (Commentary)
19	Source Tissue
8	Specific Activity [micromol/min/mg]
109	Substrates and Products (Substrate)
21	Subunits
67	Synonyms
6	Temperature Optimum [°C]
2	Temperature Range [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Purification

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Purification on EC 3.4.22.35 - Histolysain

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Entamoeba histolytica

-

30316, 30320, 30323, 30325

15fold, to homogeneity

Entamoeba histolytica

-

651056

Ni-Sepharose column chromatography

Entamoeba histolytica

-

731356

Ni2+-NTA resin column chromatography, and gel filtration

Entamoeba histolytica

C4LSI6

732864

partial

Entamoeba histolytica

-

30319

recombinant His-tagged enzyme from Escherichia coli

Entamoeba histolytica

-

653261

recombinant His-tagged full-length EhCP112 zymogen, containing the signal peptide and the pro-peptide, from Escherichia coli strain BL21 by nickel affinity chromatography

Entamoeba histolytica

-

668210

recombinant His-tagged inactive pro-enzyme to homogeneity

Entamoeba histolytica

-

653742

recombinant protein using His-tag under denaturating conditions

2 entries

recombinant refolded His-tagged enzyme by nickel affinity chromatography

Entamoeba histolytica

-

701062

to homogeneity

2 entries

recombinant protein using His-tag under denaturating conditions

Entamoeba histolytica

-

683417, 683468

recombinant protein using His-tag under denaturating conditions

Entamoeba histolytica

Q01957, Q01958

683468

to homogeneity

Entamoeba histolytica

-

668769

to homogeneity

Entamoeba histolytica

Q95030

653256