3.4.22.35: Histolysain

This is an abbreviated version!
For detailed information about Histolysain, go to the full flat file.

Reaction

hydrolysis of proteins, including basement membrane collagen and azocasein. Preferential cleavage: Arg-Arg-/- in small molecule substrates including Z-Arg-Arg-/-NHMec =

Synonyms

Amebapain, amoebapain, CP1, CP2, CP5, CP6, cysteine protease, cysteine protease 1, cysteine protease 2, cysteine protease 5, cysteine protease 6, cysteine proteinase 1, cysteine proteinase 5, EhCP1, EhCP112, EhCP2, EhCP5, Entamoeba histolytica 112kDa adhesin, Entamoeba histolytica cysteine protease, Entamoeba histolytica cysteine proteinase, Entamoeba histolytica cysteine proteinase 5, Entamoeba histolytica cysteine-proteinase, Entamoeba histolytica neutral thiol proteinase, Histolysin, More, PCP5, ppEhCP-B9, Proteinase, Entamoeba histolytica cysteine

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.35 Histolysain

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Results	in table
7	Activating Compound
8	AA Sequence
1	CAS Registry Number
12	Cloned(Commentary)
5	Expression
4	General Information
1	General Stability
44	Inhibitors
12	Ki Value [mM]
2	KM Value [mM]
17	Localization
15	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
78	Organism
13	pH Optimum
5	pH Range
1	pH Stability
3	pI Value
3	Posttranslational Modification
1	Protein Variants
16	Purification (Commentary)
3	Reaction
8	Reaction Type
41	Reference
6	Renatured (Commentary)
19	Source Tissue
8	Specific Activity [micromol/min/mg]
109	Substrates and Products (Substrate)
21	Subunits
67	Synonyms
6	Temperature Optimum [°C]
2	Temperature Range [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Posttranslational Modification

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Posttranslational Modification on EC 3.4.22.35 - Histolysain

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POSTTRANSLATIONAL MODIFICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

no glycoprotein

Entamoeba histolytica

-

no N-glycosylation

668769

proteolytic modification

Entamoeba histolytica

-

the recombinant enzyme needs to be refolded and processed from the inactive precursor, ppEhCP112, to a 35.5 kDa mature and active enzyme, EhCP112. The thiol specific inhibitor E-64, but not serine or aspartic proteinase inhibitors arrests this activation process

701062

additional information

Entamoeba histolytica

-

processing of the inactive pro-enzyme to the mature active form by treatment with 10 mM DTT and 0.04% SDS at 37°C and pH 8.8, SDS can be substituted by Triton X-100 and octylglucoside but these are 3fold less efficient

653742