3.4.22.35: Histolysain

This is an abbreviated version!
For detailed information about Histolysain, go to the full flat file.

Reaction

hydrolysis of proteins, including basement membrane collagen and azocasein. Preferential cleavage: Arg-Arg-/- in small molecule substrates including Z-Arg-Arg-/-NHMec =

Synonyms

Amebapain, amoebapain, CP1, CP2, CP5, CP6, cysteine protease, cysteine protease 1, cysteine protease 2, cysteine protease 5, cysteine protease 6, cysteine proteinase 1, cysteine proteinase 5, EhCP1, EhCP112, EhCP2, EhCP5, Entamoeba histolytica 112kDa adhesin, Entamoeba histolytica cysteine protease, Entamoeba histolytica cysteine proteinase, Entamoeba histolytica cysteine proteinase 5, Entamoeba histolytica cysteine-proteinase, Entamoeba histolytica neutral thiol proteinase, Histolysin, More, PCP5, ppEhCP-B9, Proteinase, Entamoeba histolytica cysteine

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.35 Histolysain

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Results	in table
7	Activating Compound
8	AA Sequence
1	CAS Registry Number
12	Cloned(Commentary)
5	Expression
4	General Information
1	General Stability
44	Inhibitors
12	Ki Value [mM]
2	KM Value [mM]
17	Localization
15	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
78	Organism
13	pH Optimum
5	pH Range
1	pH Stability
3	pI Value
3	Posttranslational Modification
1	Protein Variants
16	Purification (Commentary)
3	Reaction
8	Reaction Type
41	Reference
6	Renatured (Commentary)
19	Source Tissue
8	Specific Activity [micromol/min/mg]
109	Substrates and Products (Substrate)
21	Subunits
67	Synonyms
6	Temperature Optimum [°C]
2	Temperature Range [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Reference

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Reference on EC 3.4.22.35 - Histolysain

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

30316

Luaces, A.L.; Barrett, A.J.

Affinity purification and biochemical characterization of histolysin, the major cysteine proteinase of Entamoeba histolytica

Biochem. J.

250

903-909

1988

Entamoeba histolytica

2898937

brenda

30317

Navarro-Garcia, F.; Chavez-Duenas, L.; Tsutsumi, V.; Posadas del Rio, F.; Lopez-Revilla, R.

Entamoeba histolytica: increase of enterotoxicity and of 53- and 75-kDa cysteine proteinases in a clone of higher virulence

Exp. Parasitol.

80

361-372

1995

Entamoeba histolytica

7729472

brenda

30318

Scholze, H.; Loehden-Bendinger, U.; Mueller, G.; Bakker-Grunwald, T.

Subcellular distribution of amebapain, the major cysteine proteinase of Entamoeba histolytica

Arch. Med. Res.

23(3)

105-108

1992

Entamoeba histolytica

1340268

brenda

30319

Scholze, H.; Werries, E.

A weakly acidic protease has a powerful proteolytic activity in Entamoeba histolytica

Mol. Biochem. Parasitol.

11

293-300

1984

Entamoeba histolytica

6087137

brenda

30320

Scholze, H.; Schulte, W.

On the specificity of a cysteine proteinase from Entamoeba histolytica

Biomed. Biochim. Acta

47

115-123

1988

Entamoeba histolytica

2902852

brenda

30321

Perez-Montfort, R.; Ostoa-Saloma, P.; Velazquez-Medina, L.; Montfort, I.; Becker, I.

Catalytic classes of proteinases of Entamoeba histolytica

Mol. Biochem. Parasitol.

26

87-97

1987

Entamoeba histolytica, Entamoeba histolytica HM 1:IMSS

2893258

brenda

30322

Schulte, W.; Sholze, H.; Werries, E.

Specificity of a cysteine proteinase of Entamoeba histolytica towards the alpha 1-CB2 peptide of bovine collagen type I

Mol. Biochem. Parasitol.

25

39-43

1987

Entamoeba histolytica

2890103

brenda

30323

Keene, W.E.; Petitt, M.G.; Allen, S.; McKerrow, J.H.

The major neutral proteinase of Entamoeba histolytica

J. Exp. Med.

163

536-549

1986

Entamoeba histolytica

2869098

brenda

30324

Scholze, H.; Otte, J.; Werries, E.

Cysteine proteinase of Entamoeba histolytica. II. Identification of the major split position in bovine insulin B-chain

Mol. Biochem. Parasitol.

18

113-121

1986

Entamoeba histolytica

2870431

brenda

30325

Scholze, H.; Werries, E.

Cysteine proteinase of Entamoeba histolytica. I. Partial purification and action on different enzymes

Mol. Biochem. Parasitol.

18

103-112

1986

Entamoeba histolytica

2870430

brenda

649500

Franco, E.; de Araujo Soares, R.M.; Meza, I.

Specific and reversible inhibition of Entamoeba histolytica cysteine-proteinase activities by Zn2+: implications for adhesion and cell damage

Arch. Med. Res.

30

82-88

1999

Entamoeba histolytica

10372439

brenda

651056

Spinella, S.; Levavasseur, E.; Petek, F.; Rigothier, M.C.

Purification and biochemical characterization of a novel cysteine protease of Entamoeba histolytica

Eur. J. Biochem.

266

170-180

1999

Entamoeba histolytica, Entamoeba histolytica HM1:IMSS

10542062

brenda

651493

Moncada, D.; Keller, K.; Chadee, K.

Entamoeba histolytica cysteine proteinases disrupt the polymeric structure of colonic mucin and alter its protective function

Infect. Immun.

71

838-844

2003

Entamoeba histolytica

12540564

brenda

653256

Jacobs, T.; Bruchhaus, I.; Dankebar, T.; Tannich, E.; Leippe, M.

Isolation and molecular characterization of a surface-bound proteinase of Entamoeba histolytica

Mol. Microbiol.

27

169-276

1998

Entamoeba histolytica (Q95030)

-

brenda

653261

Garcia-Rivera, G.; Rodriguez, M.A.; Ocadiz, R.; Martinez-Lopez, M.C.; Arroyo, R.; Gonzalez-Robles, A.; Orozco, E.

Entamoeba histolytica: a novel cysteine protease and an adhesin form the 112kDa surface protein

Mol. Microbiol.

33

556-568

1999

Entamoeba histolytica

10417646

brenda

653742

Hellberg, A.; Nowak, N.; Leippe, M.; Tannich, E.; Bruchhaus, I.

Recombinant expression and purification of an enzymatically active cysteine proteinase of the protozoan parasite Entamoeba histolytica

Protein Expr. Purif.

24

131-137

2002

Entamoeba histolytica

11812234

brenda

668210

Ocadiz, R.; Orozco, E.; Carrillo, E.; Quintas, L.I.; Ortega-Lopez, J.; Garcia-Perez, R.M.; Sanchez, T.; Castillo-Juarez, B.A.; Garcia-Rivera, G.; Rodriguez, M.A.

EhCP112 is an Entamoeba histolytica secreted cysteine protease that may be involved in the parasite-virulence

Cell. Microbiol.

7

221-232

2005

Entamoeba histolytica

15659066

brenda

668713

Moncada, D.; Keller, K.; Ankri, S.; Mirelman, D.; Chadee, K.

Antisense inhibition of Entamoeba histolytica cysteine proteases inhibits colonic mucus degradation

Gastroenterology

130

721-730

2006

Entamoeba histolytica

16530514

brenda

668769

Scholze, H.; Tannich, E.

Histolysain and other Entamoeba cysteine endopeptidases

Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds)

2

1164-1166

2004

Entamoeba dispar, Entamoeba histolytica

-

brenda

669261

Nowak, N.; Lotter, H.; Tannich, E.; Bruchhaus, I.

Resistance of Entamoeba histolytica to the cysteine proteinase inhibitor E64 is associated with secretion of pro-enzymes and reduced pathogenicity

J. Biol. Chem.

279

38260-38266

2004

Entamoeba histolytica

15215238

brenda

670192

Tillack, M.; Nowak, N.; Lotter, H.; Bracha, R.; Mirelman, D.; Tannich, E.; Bruchhaus, I.

Increased expression of the major cysteine proteinases by stable episomal transfection underlines the important role of EhCP5 for the pathogenicity of Entamoeba histolytica

Mol. Biochem. Parasitol.

149

58-64

2006

Entamoeba histolytica

16753229

brenda

670467

Pertuz Belloso, S.; Ostoa Saloma, P.; Benitez, I.; Soldevila, G.; Olivos, A.; Garcia-Zepeda, E.

Entamoeba histolytica cysteine protease 2 (EhCP2) modulates leucocyte migration by proteolytic cleavage of chemokines

Parasite Immunol.

26

237-241

2004

Entamoeba histolytica

15491473

brenda

670707

Lidell, M.E.; Moncada, D.M.; Chadee, K.; Hansson, G.C.

Entamoeba histolytica cysteine proteases cleave the MUC2 mucin in its C-terminal domain and dissolve the protective colonic mucus gel

Proc. Natl. Acad. Sci. USA

103

9298-9303

2006

Entamoeba histolytica

16754877

brenda

683340

Saito-Nakano, Y.; Mitra, B.N.; Nakada-Tsukui, K.; Sato, D.; Nozaki, T.

Two Rab7 isotypes, EhRab7A and EhRab7B, play distinct roles in biogenesis of lysosomes and phagosomes in the enteric protozoan parasite Entamoeba histolytica

Cell. Microbiol.

9

1796-1808

2007

Entamoeba histolytica

17359234

brenda

683341

Mitra, B.N.; Saito-Nakano, Y.; Nakada-Tsukui, K.; Sato, D.; Nozaki, T.

Rab11B small GTPase regulates secretion of cysteine proteases in the enteric protozoan parasite Entamoeba histolytica

Cell. Microbiol.

9

2112-2125

2007

Entamoeba histolytica, Entamoeba histolytica (Q01957), Entamoeba histolytica (Q01958)

17441984

brenda

683417

Melendez-Lopez, S.G.; Herdman, S.; Hirata, K.; Choi, M.H.; Choe, Y.; Craik, C.; Caffrey, C.R.; Hansell, E.; Chavez-Munguia, B.; Chen, Y.T.; Roush, W.R.; McKerrow, J.; Eckmann, L.; Guo, J.; Stanley, S.L.; Reed, S.L.

Use of recombinant Entamoeba histolytica cysteine proteinase 1 to identify a potent inhibitor of amebic invasion in a human colonic model

Eukaryot. Cell

6

1130-1136

2007

Entamoeba histolytica, Entamoeba histolytica HM1:IMSS

17513563

brenda

683468

Sato, D.; Nakada-Tsukui, K.; Okada, M.; Nozaki, T.

Two cysteine protease inhibitors, EhICP1 and 2, localized in distinct compartments, negatively regulate secretion in Entamoeba histolytica

FEBS Lett.

580

5306-5312

2006

Entamoeba histolytica, Entamoeba histolytica (Q01957), Entamoeba histolytica (Q01958)

16979632

brenda

701062

Quintas-Granados, L.I.; Orozco, E.; Brieba, L.G.; Arroyo, R.; Ortega-Lopez, J.

Purification, refolding and autoactivation of the recombinant cysteine proteinase EhCP112 from Entamoeba histolytica

Protein Expr. Purif.

63

26-32

2009

Entamoeba histolytica

18824105

brenda

708440

Freitas, M.A.; Fernandes, H.C.; Calixto, V.C.; Martins, A.S.; Silva, E.F.; Pesquero, J.L.; Gomes, M.A.

Entamoeba histolytica: cysteine proteinase activity and virulence. Focus on cysteine proteinase 5 expression levels

Exp. Parasitol.

122

306-309

2009

Entamoeba histolytica

19389401

brenda

717635

Casados-Vazquez, L.E.; Lara-Gonzalez, S.; Brieba, L.G.

Crystal structure of the cysteine protease inhibitor 2 from Entamoeba histolytica: functional convergence of a common protein fold

Gene

471

45-52

2011

Entamoeba histolytica, Entamoeba histolytica HM1:IMSS

20951777

brenda

717810

Hou, Y.; Mortimer, L.; Chadee, K.

Entamoeba histolytica cysteine proteinase 5 binds integrin on colonic cells and stimulates NFkappaB-mediated pro-inflammatory responses

J. Biol. Chem.

285

35497-35504

2010

Entamoeba histolytica, Entamoeba histolytica HM1:IMSS

20837477

brenda

731356

Zamudio-Prieto, O.; Benitez-Cardoza, C.; Arroyo, R.; Ortega-Lopez, J.

Conformational changes induced by detergents during the refolding of chemically denatured cysteine protease ppEhCP-B9 from Entamoeba histolytica

Biochim. Biophys. Acta

1844

1299-1306

2014

Entamoeba histolytica

24769005

brenda

732605

Lee, Y.A.; Nam, Y.H.; Min, A.; Kim, K.A.; Nozaki, T.; Saito-Nakano, Y.; Mirelman, D.; Shin, M.H.

Entamoeba histolytica-secreted cysteine proteases induce IL-8 production in human mast cells via a PAR2-independent mechanism

Parasite

21

1-1

2014

Entamoeba histolytica

24502918

brenda

732864

Ghosh, A.; Raha, S.

Molecular and functional characterisation of a stress responsive cysteine protease, EhCP6 from Entamoeba histolytica

Protein Expr. Purif.

109

55-61

2015

Entamoeba histolytica (C4LSI6), Entamoeba histolytica, Entamoeba histolytica HM1:IMSS (C4LSI6)

25680771

brenda

Entries 1 - 20 of 34

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