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Literature summary for 3.4.22.35 extracted from
- Conformational changes induced by detergents during the refolding of chemically denatured cysteine protease ppEhCP-B9 from Entamoeba histolytica (2014), Biochim. Biophys. Acta, 1844, 1299-1306.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli C43 cells | Entamoeba histolytica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
x * 47000, SDS-PAGE | Entamoeba histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose column chromatography | Entamoeba histolytica |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| 2 mM SDS is the only surfactant that is effective at promoting the refolding and activation of the enzyme | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gelatin + H2O | - |
Entamoeba histolytica | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, SDS-PAGE | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ppEhCP-B9 | - |
Entamoeba histolytica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is highly involved in the virulence of Entamoeba histolytica | Entamoeba histolytica |
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