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dimer
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dynamic light scattering and gel filtration
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x * 24600, SDS-PAGE
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x * 27000, recombinant detagged enzyme, SDS-PAGE, x * 27218, sequence calculation
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x * 27000, recombinant detagged enzyme, SDS-PAGE, x * 27218, sequence calculation
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x * 30000-34000, SDS-PAGE
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x * 32000, about, sequence calculation
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x * 60000, recombinant N-terminally GST-tagged enzyme, SDS-PAGE, x * 32000, detagged enzyme, SDS-PAGE
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x * 32000, about, sequence calculation
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x * 27700, about, sequence calculation
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x * 60000, recombinant enzyme, SDS-PAGE
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x * about 28000, SDS-PAGE
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x * 30000-34000, SDS-PAGE
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x * about 28000, SDS-PAGE
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x * 30000, isoform UNG1, SDS-PAGE
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x * 36000, isoform UNG2, SDS-PAGE
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x * 24000, SDS-PAGE
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x * 27604, N-terminally His-tagged enzyme, sequence calculation
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x * 27604, N-terminally His-tagged enzyme, sequence calculation
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x * 30000, isoform UNG1, SDS-PAGE
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x * 36000, isoform UNG2, SDS-PAGE
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x * 28500, recombinant enzyme, SDS-PAGE
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x * 28500, recombinant enzyme, SDS-PAGE
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x * 25000, SDS-PAGE
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x * 28000, recombinant His6-tagged UDGb, SDS-PAGE
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x * 25000, SDS-PAGE
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x * 37000, recombinant enzyme, SDS-PAGE
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x * 25601, calculated from sequence
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x * 20578, sequence calculation
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x * 31000, SMUG1, SDS-PAGE
monomer
1 * 25068, calculated from amino acid sequence
monomer
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1 * 25068, calculated from amino acid sequence
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additional information
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asUNG structure homology modelling based on the crystal structure of vcUNG from Vibrio cholerae, asUNG possesses typical cold-adapted features compared with vcUNG, overview
additional information
peptide mapping
additional information
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peptide mapping
additional information
type 1 UNG structure analysis and comparison with the human and Escherichia coli enzymes, structure-activity relationship, overview
additional information
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type 1 UNG structure analysis and comparison with the human and Escherichia coli enzymes, structure-activity relationship, overview
additional information
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primary and secondary structure, and structural studies of ligand complexes, overview
additional information
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structure comparison to the human and Herpes simplex virus enzymes, overview
additional information
modeling of thymine in the active site pocket of Ung, overview
additional information
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the structural fold of UNG proteins is based on four beta-sheets sandwiched between two pairs of alpha-helices, the surface of the protein globule is traversed by a shallow and narrow positively charged groove, where substrate DNA binds. This groove harbors the enzyme's active site, which in turn contains a deep pocket that accommodates the uracil base, conformational change upon substrate binding, overall structure and conserved structural motifs, structure-function relationship, overview
additional information
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structure comparison to the human and Herpes simplex virus enzymes, overview
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additional information
mutational analysis and molecular dynamics simulations of SMUG1 identify important structural determinants in conserved motifs 1 and 2 for XDG and UDG activities
additional information
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mutational analysis and molecular dynamics simulations of SMUG1 identify important structural determinants in conserved motifs 1 and 2 for XDG and UDG activities
additional information
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mutational analysis and molecular dynamics simulations of SMUG1 identify important structural determinants in conserved motifs 1 and 2 for XDG and UDG activities
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additional information
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primary and secondary structure, and structural studies of ligand complexes, overview
additional information
structure of wild-type enzyme in complex with DNA, modelling, overview
additional information
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structure of wild-type enzyme in complex with DNA, modelling, overview
additional information
three-dimensional structure and molecular modelling
additional information
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three-dimensional structure and molecular modelling
additional information
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homology modelling of the structure of hSMUG1
additional information
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the structural fold of UNG proteins is based on four beta-sheets sandwiched between two pairs of alpha-helices, the surface of the protein globule is traversed by a shallow and narrow positively charged groove, where substrate DNA binds. This groove harbors the enzyme's active site, which in turn contains a deep pocket that accommodates the uracil base, conformational change upon substrate binding, overall structure and conserved structural motifs, structure-function relationship, overview
additional information
primary and secondary structure, and structural studies of ligand complexes, overview
additional information
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UDG contains a helix-hairpin-helix motif and Gly/Pro rich loop in the GPD region important for catalytic activity
additional information
UDG contains a helix-hairpin-helix motif and Gly/Pro rich loop in the GPD region important for catalytic activity
additional information
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the initiating amino acid, formyl-methionine is cleaved from the mature UDG protein
additional information
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the initiating amino acid, formyl-methionine is cleaved from the mature UDG protein
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additional information
family 5 UDGB secondary structure elements, overview
additional information
Q7WYV4
the UDG possesses a [4Fe-4S] cluster, distant from the active site, which interacts with loop structures and is unessential to the activity but necessary for stabilizing the loop structures, salt-bridges and ion pairs on the molecular surface and the presence of proline on loops and turns contribute to the enzyme's thermostability
additional information
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family 5 UDGB secondary structure elements, overview
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additional information
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the UDG possesses a [4Fe-4S] cluster, distant from the active site, which interacts with loop structures and is unessential to the activity but necessary for stabilizing the loop structures, salt-bridges and ion pairs on the molecular surface and the presence of proline on loops and turns contribute to the enzyme's thermostability
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additional information
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the enzyme differs from UDGs of other species in its primary, secondary and tertiary structure, through modifications of otherwise conserved active site motifs, assembly and protein-protein interactions, overview
additional information
UNG structure modelling, overview
additional information
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UNG structure modelling, overview