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Literature summary for 3.2.2.27 extracted from

  • Knaevelsrud, I.; Slupphaug, G.; Leiros, I.; Matsuda, A.; Ruoff, P.; Bjelland, S.
    Opposite-base dependent excision of 5-formyluracil from DNA by hSMUG1 (2009), Int. J. Radiat. Biol., 85, 413-420.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters for the opposite-base dependent excision of 5-formyluracil from DNA by hSMUG1 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens hSMUG1 shows excision activity for 5-formyluracil, a major thymine lesion formed by ionizing radiation, opposite all normal bases in DNA ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fU-containing 10 nucleotide DNA sequence 5'-GGAGAfUCTCC-3' with opposing C, T, A, or G + H2O
-
Homo sapiens ?
-
?
additional information hSMUG1 shows excision activity for 5-formyluracil, a major thymine lesion formed by ionizing radiation, opposite all normal bases in DNA Homo sapiens ?
-
?
additional information excision of 5-formyluracil, fU, from fU-containing 10 nucleotide DNA sequence 5'-GGAGAfUCTCC-3'. Substrate-binding pocket of hSMUG1 and its interactions with uracil and fU, base-pairing properties of fU residues in DNA, structures, overview Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More homology modelling of the structure of hSMUG1 Homo sapiens

Synonyms

Synonyms Comment Organism
single-strand-selective monofunctional uracil-DNA glycosylase
-
Homo sapiens
SMUG1
-
Homo sapiens
UNG2
-
Homo sapiens
uracil-N-glycosylase 2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0002 0.0003 fU-containing 10 nucleotide DNA sequence 5'-GGAGAfUCTCC-3' with opposing C, T, A, or G pH 7.5 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens