3.2.2.27: uracil-DNA glycosylase

This is an abbreviated version!
For detailed information about uracil-DNA glycosylase, go to the full flat file.

Reaction

Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil =

Synonyms

5-formyluracil-DNA glycosylase, AFUDG, Afung protein, Blr0248, DNA N-glycosylase, dr0689, DR_0968, endonuclease VIII, family 1 uracil N-glycosylase, family 4 UDGa, family 4 uracil-DNA glycosylase, HMUDG, mismatch-specific uracil-DNA glycosylase, MjUDG, More, MtuNei1, MUG, Pa-UDG, PF1385, PfuUDG, single-strand selective monofunctional uracil-DNA glycosylase, single-strand selective uracil DNA glycosylase, single-strand-selective mono-functional uracil-DNA glycosylase, single-strand-selective monofunctional uracil DNA glycosylase, single-strand-selective monofunctional uracil-DNA glycosylase, single-stranded monofunctional uracil DNA N-glycosylase, SMUG1, SMUG1-like glycosylase, STK_22380, Ta0477, TD12-encoded protein, Thd1p, Thp1p, TMUDG, type 1 UDG, UDG, UDG1, UDGa, UdgB, UDGIV, UDGV, UL114, UL2, UNG, UNG-1, Ung-type uracil glycosylase, UNG1, UNG2, Unga, uracil DNA glycosylase, uracil DNA glycosylase 2, uracil DNA-glycosylase, uracil DNA-glycosylase 2, uracil-DNA glycosylase, uracil-DNA glycosylase 2, uracil-DNA glycosylase a, uracil-DNA N-glycosylase, uracil-N-glycosylase 2

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.2 Hydrolysing N-glycosyl compounds

                3.2.2.27 uracil-DNA glycosylase

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Results	in table
9	Activating Compound
54789	AA Sequence
12	Application
1	CAS Registry Number
74	Cloned(Commentary)
1	Cofactor
24	Crystallization (Commentary)
425	Disease/ Diagnostics
3	Expression
35	General Information
4	General Stability
1	IC50 Value
66	Inhibitors
42	kcat/KM [mM/s]
4	Ki Value [mM]
90	KM Value [mM]
25	Localization
12	Metals/Ions
24	Molecular Weight [Da]
161	Natural Substrates/ Products (Substrates)
568	Organism
119	PDB ID
57	pH Optimum
9	pH Range
1	pH Stability
3	pI Value
2	Posttranslational Modification
127	Protein Variants
64	Purification (Commentary)
12	Reaction
152	Reference
1	Renatured (Commentary)
53	Source Tissue
27	Specific Activity [micromol/min/mg]
326	Substrates and Products (Substrate)
61	Subunits
288	Synonyms
1	Systematic Name
46	Temperature Optimum [°C]
10	Temperature Range [°C]
29	Temperature Stability [°C]
76	Turnover Number [1/s]

Inhibitors

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of the Enzyme Summary Page.

Inhibitors on EC 3.2.2.27 - uracil-DNA glycosylase

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1'-cyano-2'-deoxyuridine

2 entries

4(6)-aminouracil

Monkeypox virus

Q8V4Y4

-

690882

4-hydroxymercuribenzoate

Archaeoglobus fulgidus

O28007

1 mM, 94% inhibition

723240

4-methylindole

Homo sapiens

-

4-methylindole is an adenine isostere incapable of hydrogen bonding, and its pair with Thy is inherently prone to spontaneous opening, structure of hUNGin a complex with DNA containing a Thy:4-methylindole pair, a DNA mimic, overview

710029

5-Azauracil

Monkeypox virus

Q8V4Y4

strong inhibition

690882

5-halogenated uracil analogues

Monkeypox virus

Q8V4Y4

-

690882

-

Alba protein

Saccharolobus solfataricus

-

a small basic chromatin protein, repression of UDG1 activity

690803

-

apurinic/apyrimidinic site

Archaeoglobus fulgidus

O28007

the enzyme is product-inhibited by both uracil and apurinic/apyrimidinic sites

692950

-

Bacillus subtilis Ung inhibitor

Caenorhabditis elegans

-

-

694294

-

Cd2+

Listeria innocua

Q926U4

complete inhibition at 5 mM

750389

Co2+

2 entries

Cu2+

2 entries

DNA containing 1'-cyano-2'-deoxyuridine

2 entries

-

Fe2+

Listeria innocua

Q926U4

complete inhibition at 5 mM

750389

G-U dsDNA

Thermus thermophilus

Q7WYV4

product inhibition

693611

-

gentamicin

Escherichia coli

-

-

750294

MgCl2

3 entries

NaCl

2 entries

Ni2+

2 entries

Sso7d protein

Saccharolobus solfataricus

-

a small basic chromatin protein, slight repression of UDG1 activity

690803

-

UGD inhibition protein from Bacillus subtilis

Mycolicibacterium smegmatis

-

phage PBS-2-encoded uracil DNA glycosylase inhibitor, UDG forms a dissociable, activity-reduced complex with the inhibitor protein Ugi in 1:1 molar stoichiometry

692153

-

Ugi

4 entries

-

UGI inhibitor

2 entries

-

Ugi peptide

2 entries

-

Ugi protein

Homo sapiens

-

-

750113, 751736, 752261

-

UNG inhibitor Ugi

Gadus morhua

-

from the Bacillus subtilis bacteriophage PBS2

691896

-

Uracil

3 entries

uracil glycosylase inhibitor protein

Escherichia coli

-

-

750294

-

uracil-DNA glycosylase inhibitor

Mycobacterium tuberculosis

-

Ugi

749488

-

uracil-DNA glycosylase inhibitor protein

5 entries

-

Zn2+

2 entries

additional information

20 entries

-

1'-cyano-2'-deoxyuridine

Escherichia coli

-

-

708870

1'-cyano-2'-deoxyuridine

Homo sapiens

P13051

-

708870

Co2+

Listeria innocua

Q926U4

about 10% residual activity at 5 mM

750389

Co2+

Sulfolobus acidocaldarius

-

-

750695

Cu2+

Listeria innocua

Q926U4

complete inhibition at 5 mM

750389

Cu2+

Sulfolobus acidocaldarius

-

-

750695

DNA containing 1'-cyano-2'-deoxyuridine

Escherichia coli

-

CNdU, a UDG inhibitor whose respective nucleotide triphosphate is also a substrate for DNA polymerase

708870

-

DNA containing 1'-cyano-2'-deoxyuridine

Homo sapiens

P13051

CNdU, a UDG inhibitor whose respective nucleotide triphosphate is also a substrate for DNA polymerase

708870

-

MgCl2

Monkeypox virus

Q8V4Y4

the viral enzyme is strongly inhibited by physiological concentrations of NaCl and MgCl2

690882

MgCl2

Vaccinia virus

-

strong inhibition

691515

MgCl2

Vaccinia virus

-

strong inhibition at 7.5 mM

694422

NaCl

Monkeypox virus

Q8V4Y4

10fold inhibition at 10 mM, the viral enzyme is strongly inhibited by physiological concentrations of NaCl and MgCl2

690882

NaCl

Sulfolobus acidocaldarius

-

when the concentration of NaCl is higher than 50 mM, the enzymatic activity is decreased by more than 50%

750695

Ni2+

Listeria innocua

Q926U4

complete inhibition at 5 mM

750389

Ni2+

Sulfolobus acidocaldarius

-

-

750695

Ugi

Caenorhabditis elegans

-

a peptide inhibitor specific for UNG-family enzyme

708316

-

Ugi

Escherichia coli

P12295

competitive binding to wild-type and mutant enzymes, overview

710147

-

Ugi

Homo sapiens

-

UDG inhibitor protein from Bacillus subtilis

694295

-

Ugi

Mus musculus

-

a specific peptide inhibitor of UNG, inhibits class switch recombination without reducing DNA cleavage of the switch region, confirming dispensability of UNG in DNA cleavage in class switch recombination

710420

-

UGI inhibitor

Homo sapiens

Q53HV7

-

691932

-

UGI inhibitor

Xenopus laevis

-

-

691932

-

Ugi peptide

Homo sapiens

P22674

-

694122

-

Ugi peptide

Homo sapiens

-

-

732799, 732826

-

Uracil

Archaeoglobus fulgidus

O28007

the enzyme is product-inhibited by both uracil and apurinic/apyrimidinic sites; UDG is product-inhibited by both uracil and apurinic/apyrimidinic sites

692950

Uracil

Archaeoglobus fulgidus

O28007

5 mM, 51% inhibition

723240

Uracil

Bradyrhizobium diazoefficiens

Q89XR0

free uracil causes a 5fold reduction in enzyme activity at 10 mM concentration

751741

uracil-DNA glycosylase inhibitor protein

Pyrobaculum islandicum

-

-

725030

-

uracil-DNA glycosylase inhibitor protein

Pyrococcus furiosus

-

-

725030

-

uracil-DNA glycosylase inhibitor protein

Saccharolobus shibatae

-

-

725030

-

uracil-DNA glycosylase inhibitor protein

Saccharolobus solfataricus

-

-

725030

-

uracil-DNA glycosylase inhibitor protein

Thermotoga maritima

-

-

725030

-

Zn2+

Listeria innocua

Q926U4

complete inhibition at 5 mM

750389

Zn2+

Sulfolobus acidocaldarius

-

-

750695

additional information

Archaeoglobus fulgidus

-

UDG is product-inhibited by both uracil and apurinic/apyrimidinic sites

692950

-

additional information

Archaeoglobus fulgidus

O28007

UDG is product-inhibited by both uracil and apurinic/apyrimidinic sites

692950

-

additional information

Bradyrhizobium diazoefficiens

Q89XR0

not inhibited by ctcaagtg-tetrahydrofuran-aggcatgcaagagct, DNA with abasic site, and Ugi protein

751741

-

additional information

Bradyrhizobium diazoefficiens

-

not inhibited by ctcaagtg-tetrahydrofuran-aggcatgcaagagct, DNA with abasic site, and Ugi protein

751741

-

additional information

Chlamydia pneumoniae

Q9Z7D3

free uracil and abasic sites inhibit the enzyme reaction

694957

-

additional information

Chlamydia pneumoniae

-

free uracil and abasic sites inhibit the enzyme reaction

694957

-

additional information

Escherichia coli

P12295

association of linker histone reduces activity of UDG at selected sites near where the globular domain of H1 is proposed to bind to the nucleosome as well as within the extra-core DNA

709131

-

additional information

Homo sapiens

Q53HV7

design of mechanism-based inhibitors, overview

691932

-

additional information

Homo sapiens

-

design of mechanism-based inhibitors, overview

691932

-

additional information

Homo sapiens

Q53HV7

no inhibition of SMUG1 by uracil glycosylase inhibitor

693231

-

additional information

Homo sapiens

-

no inhibition of SMUG1 by uracil glycosylase inhibitor

693231

-

additional information

Monkeypox virus

Q8V4Y4

inhibitor screening, no inhibition by uracil

690882

-

additional information

Monkeypox virus

-

inhibitor screening, no inhibition by uracil

690882

-

additional information

Pyrobaculum aerophilum

Q8ZXE0

no inhibition by UGD inhibition protein from Bacillus subtilis-peptide

692065

-

additional information

Pyrobaculum aerophilum

Q8ZYS2

no inhibition by UGD inhibition protein from Bacillus subtilis-peptide

692065

-

additional information

Pyrobaculum aerophilum

-

no inhibition by UGD inhibition protein from Bacillus subtilis-peptide

692065

-

additional information

Thermotoga maritima

-

weak product inhibition of TMUDG by uracil

694297

-

additional information

Vaccinia virus

-

no inhibition of Vaccinia virus UDG by uracil-DNA glycosylase inhibitor protein, Ugi, from Bacillus subtilis bacteriophages PBS1 and PBS2

691515

-

additional information

Vaccinia virus

-

no inhibition by uracil-DNA glycosylase inhibitor, UGI, from Bacillus subtilis bacteriophages PBS1 and PBS2

694422

-

additional information

Xenopus laevis

-

design of mechanism-based inhibitors, overview

691932

-