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0.015
5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
0.04
5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
0.098
5-hydroxymethyluracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hSMUG1
-
0.000072 - 0.23
double stranded DNA containing G-U mismatch
-
0.0002 - 0.0003
fU-containing 10 nucleotide DNA sequence 5'-GGAGAfUCTCC-3' with opposing C, T, A, or G
-
pH 7.5
-
1.45 - 30.3
uracil-containing calf thymus DNA
-
0.33 - 0.85
uracil-containing double-stranded DNA
-
0.18 - 0.27
uracil-containing single-stranded DNA
-
0.0175 - 15.58
uracil-mismatched double-stranded DNA
-
5.9
uracil-mismatched double-stranded DNA with A-U mismatch +
pH 8.0, 37°C, recombinant wild-type enzyme
-
0.052 - 2.28
uracil-mismatched double-stranded DNA with U-A mismatch
-
0.0014 - 5.57
uracil-mismatched double-stranded DNA with U-G mismatch
-
0.083 - 46.1
uracil-mismatched single-stranded DNA
-
additional information
additional information
-
0.000072
double stranded DNA containing G-U mismatch
mutant enzyme H155S, at pH 7.6 and 60°C
-
0.0075
double stranded DNA containing G-U mismatch
mutant enzyme E41Q/G42D, at pH 7.6 and 60°C
-
0.025
double stranded DNA containing G-U mismatch
mutant enzyme N89A, at pH 7.6 and 60°C
-
0.23
double stranded DNA containing G-U mismatch
wild type enzyme, at pH 7.6 and 60°C
-
154
dUMP DNA
pH 7.5, recombinant mutant V90R
-
174
dUMP DNA
-
pH 7.5, recombinant wild-type enzyme
-
293
dUMP DNA
pH 7.5, recombinant wild-type enzyme
-
821
dUMP DNA
pH 7.5, recombinant mutant H194R
-
1.45
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 15°C
-
1.65
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 15°C
-
2
uracil-containing calf thymus DNA
-
mutant enzyme G183D, at pH 7.5 and 15°C
-
2.37
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 15°C
-
4.07
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 22°C
-
4.08
uracil-containing calf thymus DNA
-
mutant enzyme G183D, at pH 7.5 and 22°C
-
4.55
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 22°C
-
4.65
uracil-containing calf thymus DNA
-
wild type enzyme, at pH 7.5 and 15°C
-
4.67
uracil-containing calf thymus DNA
-
mutant enzyme G183D/R302K, at pH 7.5 and 22°C
-
5.33
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 22°C
-
7.73
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 37°C
-
9.83
uracil-containing calf thymus DNA
-
mutant enzyme G183D, at pH 7.5 and 37°C
-
10.28
uracil-containing calf thymus DNA
-
wild type enzyme, at pH 7.5 and 22°C
-
10.78
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 37°C
-
13.6
uracil-containing calf thymus DNA
-
mutant enzyme G183D/R302K, at pH 7.5 and 37°C
-
16.85
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 37°C
-
20.68
uracil-containing calf thymus DNA
-
wild type enzyme, at pH 7.5 and 37°C
-
30.3
uracil-containing calf thymus DNA
-
mutant enzyme G183D/R302K, at pH 7.5 and 15°C
-
0.33
uracil-containing double-stranded DNA
-
pH 7.5, 52°C, wild-type enzyme
-
0.85
uracil-containing double-stranded DNA
-
pH 7.5, 52°C, mutant enzyme K100A
-
0.18
uracil-containing single-stranded DNA
-
pH 7.5, 52°C, wild-type enzyme
-
0.27
uracil-containing single-stranded DNA
-
pH 7.5, 52°C, mutant enzyme K100A
-
0.0175
uracil-mismatched double-stranded DNA
pH 7.5, 70°C
-
0.02
uracil-mismatched double-stranded DNA
pH 7.5, 80°C
-
0.042
uracil-mismatched double-stranded DNA
pH 7.5, 37°C, excision of a single uracil residue from U-A, recombinant enzyme
-
0.042
uracil-mismatched double-stranded DNA
pH 8.0, 37°C, recombinant mutant D77N
-
0.048
uracil-mismatched double-stranded DNA
pH 7.5, 80°C, recombinant enzyme
-
0.053
uracil-mismatched double-stranded DNA
pH 8.0, 37°C, recombinant mutant H200Q
-
0.3
uracil-mismatched double-stranded DNA
pH 7.0, 37°C, excision of a single uracil residue from U-G, recombinant enzyme
-
0.55
uracil-mismatched double-stranded DNA
pH 7.5, 37°C
-
0.73
uracil-mismatched double-stranded DNA
pH 8.0, 37°C, recombinant mutant A205S
-
0.92
uracil-mismatched double-stranded DNA
pH 7.8, 70°C
-
0.967
uracil-mismatched double-stranded DNA
pH 7.5, 37°C, excision of a single uracil residue from U-G, recombinant enzyme
-
1.8
uracil-mismatched double-stranded DNA
pH 7.5, 80°C, cell extract (wild-type enzyme)
-
1.83
uracil-mismatched double-stranded DNA
pH 7.5, 37°C, excision of a single uracil residue from U-T, recombinant enzyme
-
2.18
uracil-mismatched double-stranded DNA
pH 7.5, 37°C, excision of a single uracil residue from PS-U, recombinant enzyme
-
2.38
uracil-mismatched double-stranded DNA
pH 7.5, 37°C, excision of a single uracil residue from U-C, recombinant enzyme
-
4.67
uracil-mismatched double-stranded DNA
pH 7.0, 37°C, excision of a single uracil residue from PS-G, recombinant enzyme
-
15.58
uracil-mismatched double-stranded DNA
pH 8.0, 37°C, recombinant enzyme
-
0.052
uracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
2.28
uracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hUNG2
-
0.0014
uracil-mismatched double-stranded DNA with U-G mismatch
-
-
-
0.014
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 37°C, wild-type enzyme
-
0.055
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
5.57
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hUNG2
-
0.083
uracil-mismatched single-stranded DNA
pH 7.5, 95°C
-
0.17
uracil-mismatched single-stranded DNA
Q7WYV4
pH 7.5, 37°C, family 4 UDG
-
0.29
uracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hSMUG1
-
0.55
uracil-mismatched single-stranded DNA
pH 7.8, 95°C
-
0.917
uracil-mismatched single-stranded DNA
pH 7.5, 37°C
-
2.4
uracil-mismatched single-stranded DNA
wild type enzyme, at pH 7.5 and 15°C
-
2.5
uracil-mismatched single-stranded DNA
-
-
-
4.7
uracil-mismatched single-stranded DNA
-
wild type enzyme, at pH 7.5 and 15°C
-
7.7
uracil-mismatched single-stranded DNA
mutant enzyme G183G, at pH 7.5 and 37°C
-
9.8
uracil-mismatched single-stranded DNA
-
mutant enzyme G183D, at pH 7.5 and 37°C
-
10.8
uracil-mismatched single-stranded DNA
wild type enzyme, at pH 7.5 and 37°C
-
13.6
uracil-mismatched single-stranded DNA
-
mutant enzyme G183D/R302K, at pH 7.5 and 37°C
-
16.9
uracil-mismatched single-stranded DNA
mutant enzyme G183G/K302R, at pH 7.5 and 37°C
-
20.7
uracil-mismatched single-stranded DNA
-
wild type enzyme, at pH 7.5 and 37°C
-
46.1
uracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hUNG2
-
additional information
additional information
excises 0.04 uracils per sec from uracil-mismatched oligonucleotide
-
additional information
additional information
-
excises 0.04 uracils per sec from uracil-mismatched oligonucleotide
-
additional information
additional information
kinetic parameters determined for Afung protein suggest a significantly lower level of enzymatic uracil release in Aeroglobus fulgidus as compared to the mesophilic Escherichia coli
-
additional information
additional information
-
kinetic parameters determined for Afung protein suggest a significantly lower level of enzymatic uracil release in Aeroglobus fulgidus as compared to the mesophilic Escherichia coli
-
additional information
additional information
-
rate constants of uracil excision determined for AFUDG and pseudo-FCL mutants using single-stranded uracil containing or double-stranded U:G base pair-containing substrates under single-turnover conditions
-