Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.27 extracted from

  • Duraffour, S.; Ishchenko, A.A
    Saparbaev, M.; Crance, J.-M.; Garin, D.: Substrate specificity of homogeneous Monkeypox virus uracil-DNA glycosylase (2007), Biochemistry, 46, 11874-11881.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
DTT
-
Monkeypox virus
EDTA
-
Monkeypox virus

Application

Application Comment Organism
medicine inhibition of UNG is a rational therapeutic strategy for infections with poxviruses Monkeypox virus

Cloned(Commentary)

Cloned (Comment) Organism
gene E4R, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Monkeypox virus

Inhibitors

Inhibitors Comment Organism Structure
4(6)-aminouracil
-
Monkeypox virus
5-Azauracil strong inhibition Monkeypox virus
5-halogenated uracil analogues
-
Monkeypox virus
MgCl2 the viral enzyme is strongly inhibited by physiological concentrations of NaCl and MgCl2 Monkeypox virus
additional information inhibitor screening, no inhibition by uracil Monkeypox virus
NaCl 10fold inhibition at 10 mM, the viral enzyme is strongly inhibited by physiological concentrations of NaCl and MgCl2 Monkeypox virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Monkeypox virus
0.000015
-
uracil-mismatched double-stranded DNA pH 7.0, 37°C, excision of a single uracil residue from PS-G, recombinant enzyme Monkeypox virus
0.00003
-
uracil-mismatched double-stranded DNA pH 7.0, 37°C, excision of a single uracil residue from U-G, recombinant enzyme Monkeypox virus
0.001
-
uracil-mismatched double-stranded DNA pH 7.5, 37°C, excision of a single uracil residue from U-A, recombinant enzyme Monkeypox virus
0.001
-
uracil-mismatched double-stranded DNA pH 7.5, 37°C, excision of a single uracil residue from U-G, recombinant enzyme Monkeypox virus
0.0014
-
uracil-mismatched double-stranded DNA pH 7.5, 37°C, excision of a single uracil residue from U-T, recombinant enzyme Monkeypox virus
0.0016
-
uracil-mismatched double-stranded DNA pH 7.5, 37°C, excision of a single uracil residue from U-C, recombinant enzyme Monkeypox virus
0.0017
-
uracil-mismatched double-stranded DNA pH 7.5, 37°C, excision of a single uracil residue from PS-U, recombinant enzyme Monkeypox virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27604
-
x * 27604, N-terminally His-tagged enzyme, sequence calculation Monkeypox virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O Monkeypox virus under physiological conditions of 60 mM NaCl, pH 7.5, increasing amounts of viral UNG cleave both 45mer U-G and PS-U oligonucleotides. Monkeypox virus, which occurs naturally in Africa, can cause a smallpoxlike disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview uracil + double-stranded DNA with abasic site
-
?
uracil-mismatched double-stranded DNA + H2O Monkeypox virus Zaire 1996 under physiological conditions of 60 mM NaCl, pH 7.5, increasing amounts of viral UNG cleave both 45mer U-G and PS-U oligonucleotides. Monkeypox virus, which occurs naturally in Africa, can cause a smallpoxlike disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview uracil + double-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O Monkeypox virus Monkeypox virus, which occurs naturally in Africa, can cause a smallpox-like disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview uracil + single-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O Monkeypox virus Zaire 1996 Monkeypox virus, which occurs naturally in Africa, can cause a smallpox-like disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview uracil + single-stranded DNA with abasic site
-
?

Organism

Organism UniProt Comment Textmining
Monkeypox virus Q8V4Y4 gene E4R
-
Monkeypox virus Zaire 1996 Q8V4Y4 gene E4R
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Monkeypox virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O under physiological conditions of 60 mM NaCl, pH 7.5, increasing amounts of viral UNG cleave both 45mer U-G and PS-U oligonucleotides. Monkeypox virus, which occurs naturally in Africa, can cause a smallpoxlike disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview Monkeypox virus uracil + double-stranded DNA with abasic site
-
?
uracil-mismatched double-stranded DNA + H2O cleavage of a duplex oligonucleotide containing a single lesion at a defined position. The viral mpUNG protein excises uracil and prefers the U-G pair over the U-A pair and does not excise oxidized bases Monkeypox virus uracil + double-stranded DNA with abasic site
-
?
uracil-mismatched double-stranded DNA + H2O under physiological conditions of 60 mM NaCl, pH 7.5, increasing amounts of viral UNG cleave both 45mer U-G and PS-U oligonucleotides. Monkeypox virus, which occurs naturally in Africa, can cause a smallpoxlike disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview Monkeypox virus Zaire 1996 uracil + double-stranded DNA with abasic site
-
?
uracil-mismatched double-stranded DNA + H2O cleavage of a duplex oligonucleotide containing a single lesion at a defined position. The viral mpUNG protein excises uracil and prefers the U-G pair over the U-A pair and does not excise oxidized bases Monkeypox virus Zaire 1996 uracil + double-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O Monkeypox virus, which occurs naturally in Africa, can cause a smallpox-like disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview Monkeypox virus uracil + single-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O cleavage of a single-stranded oligonucleotide containing a single lesion at a defined position, usage of single-stranded DNA oligonucleotide labeled with a 5'-fluorescein and a 3'-dabsyl. The viral mpUNG protein excises uracil preferentially from single-stranded DNA and does not excise oxidized bases Monkeypox virus uracil + single-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O Monkeypox virus, which occurs naturally in Africa, can cause a smallpox-like disease in humans. The DNA repair protein uracil-DNA glycosylase, UNG, is one of the viral enzymes important for poxvirus pathogenesis, thus inhibition of UNG is a therapeutic strategy, overview Monkeypox virus Zaire 1996 uracil + single-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O cleavage of a single-stranded oligonucleotide containing a single lesion at a defined position, usage of single-stranded DNA oligonucleotide labeled with a 5'-fluorescein and a 3'-dabsyl. The viral mpUNG protein excises uracil preferentially from single-stranded DNA and does not excise oxidized bases Monkeypox virus Zaire 1996 uracil + single-stranded DNA with abasic site
-
?

Subunits

Subunits Comment Organism
? x * 27604, N-terminally His-tagged enzyme, sequence calculation Monkeypox virus

Synonyms

Synonyms Comment Organism
UNG
-
Monkeypox virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Monkeypox virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.042
-
uracil-mismatched double-stranded DNA pH 7.5, 37°C, excision of a single uracil residue from U-A, recombinant enzyme Monkeypox virus
0.3
-
uracil-mismatched double-stranded DNA pH 7.0, 37°C, excision of a single uracil residue from U-G, recombinant enzyme Monkeypox virus
0.967
-
uracil-mismatched double-stranded DNA pH 7.5, 37°C, excision of a single uracil residue from U-G, recombinant enzyme Monkeypox virus
1.83
-
uracil-mismatched double-stranded DNA pH 7.5, 37°C, excision of a single uracil residue from U-T, recombinant enzyme Monkeypox virus
2.18
-
uracil-mismatched double-stranded DNA pH 7.5, 37°C, excision of a single uracil residue from PS-U, recombinant enzyme Monkeypox virus
2.38
-
uracil-mismatched double-stranded DNA pH 7.5, 37°C, excision of a single uracil residue from U-C, recombinant enzyme Monkeypox virus
4.67
-
uracil-mismatched double-stranded DNA pH 7.0, 37°C, excision of a single uracil residue from PS-G, recombinant enzyme Monkeypox virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Monkeypox virus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
pH profile Monkeypox virus