Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3)pLysS cells | Vaccinia virus |
Crystallization (Comment) | Organism |
---|---|
mutant enzymes R167A, E171G/S172G/P173G, and DELTAE171/DELTAS172, hanging drop vapor diffusion method, using of 0.6-1.0 M ammonium sulfate, 0.1 M HEPES buffer pH 7.4, 10% (v/v) glycerol or 16% (w/v) PEG 3350, 0.15 M potassium fluoride, 10% (v/v) glycerol, or 2.0 M lithium sulfate in 50 mM MES buffer pH 5.6, 5 mM magnesium chloride, 5% (v/v) ethylene glycol | Vaccinia virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25068 | - |
1 * 25068, calculated from amino acid sequence | Vaccinia virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched single-stranded DNA + H2O | Vaccinia virus | - |
uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | Vaccinia virus Ankara | - |
uracil + single-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vaccinia virus | Q91UM2 | - |
- |
Vaccinia virus Ankara | Q91UM2 | - |
- |
Purification (Comment) | Organism |
---|---|
TALON column chromatography and Superdex 75 gel filtration | Vaccinia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched single-stranded DNA + H2O | - |
Vaccinia virus | uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | - |
Vaccinia virus Ankara | uracil + single-stranded DNA with abasic site | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25068, calculated from amino acid sequence | Vaccinia virus |
Synonyms | Comment | Organism |
---|---|---|
UDG | - |
Vaccinia virus |
uracil DNA glycosylase | - |
Vaccinia virus |