Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
D150E | site-directed mutagenesis, inactive mutant | Methanocaldococcus jannaschii |
D150E | displays reduced activity of about 70% of the wild type value | Methanocaldococcus jannaschii |
D150W | site-directed mutagenesis, the mutant shows 70% of wild-type enzyme activity | Methanocaldococcus jannaschii |
D150W | completely lacks DNA glycosylase activity | Methanocaldococcus jannaschii |
E132K | site-directed mutagenesis, a mutation in the HhH motif with a lysine residue equivalent to Lys120 in endonuclease III leading to conversion of the enzyme into a bifunctional glycosylase/AP lyase capable of both removing uracil at a glycosylic bond and cleaving the phosphodiester backbone at an AP site. Mutant E132K catalyzes a beta-elimination reaction at the AP site via uracil excision and forms a Schiff base intermediate in the form of a protein-DNA complex | Methanocaldococcus jannaschii |
E132K | mutation converts the enzyme into a bifunctional glycosylase/AP lyase capable of both removing uracil at a glycosylic bond and cleaving the phosphodiester backbone at an apurinic/apyrimidinic site. The mutant catalyzes a beta-elimination reaction at the apurinic/apyrimidinic site via uracil excision and forms a Schiff base intermediate in the form of a protein-DNA complex | Methanocaldococcus jannaschii |
Y152E | site-directed mutagenesis, the mutant shows unaltered enzyme activity compared to the wild-type enzyme | Methanocaldococcus jannaschii |
Y152E | retains unchanged levels of uracil-DNA glycosylase activity | Methanocaldococcus jannaschii |
Y152N | site-directed mutagenesis, the mutant shows unaltered enzyme activity compared to the wild-type enzyme | Methanocaldococcus jannaschii |
Y152N | retains unchanged levels of uracil-DNA glycosylase activity | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, SDS-PAGE | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanocaldococcus jannaschii | Q58829 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58829 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded oligonucleotide + H2O | oligonucleotide duplex containing a U:T mispair | Methanocaldococcus jannaschii | uracil + double-stranded oligonucleotide with abasic site | - |
? | |
uracil-mismatched double-stranded oligonucleotide + H2O | oligonucleotide duplex containing a U:T mispair | Methanocaldococcus jannaschii DSM 2661 | uracil + double-stranded oligonucleotide with abasic site | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Methanocaldococcus jannaschii |
More | UDG contains a helix-hairpin-helix motif and Gly/Pro rich loop in the GPD region important for catalytic activity | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
MjUDG | - |
Methanocaldococcus jannaschii |
UDG | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Methanocaldococcus jannaschii |