Protein Variants | Comment | Organism |
---|---|---|
R276X | mutations at Arg276 transform uracil-DNA glycosylase into a single-stranded DNA-specific uracil-DNA glycosylase. The kcat of the R276 mutants is comparable to wild-type UNG on single-stranded DNA and differentially affected by NaCl, however, kcat on double-stranded DNA substrate is reduced 4-12-fold and decreases sharply at NaCl concentrations as low as 20 mM, the mutant proteins exhibit a 2.6 to 7.7fold reduction in affinity for a doubled-stranded oligonucleotide containing a pseudouracil residue opposite 2-aminopurine compared to the wild-type UNG | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P13051 | - |
- |
Subunits | Comment | Organism |
---|---|---|
More | structure of wild-type enzyme in complex with DNA, modelling, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
UNG | - |
Homo sapiens |