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Literature summary for 3.2.2.27 extracted from

  • Kim, G.A.; Sun Lee, M.; Sun, Y.; Loo, B.D.; Lee, J.I.; Lee, J.-H.; Kwon, S.-T.
    Characterization of cold-active uracil-DNA glycosylase from Bacillus sp. HJ171 and its use for contamination ontrol in PCR (2008), Appl. Microbiol. Biotechnol., 80, 785-794.
    View publication on PubMed
Search for more literature for 3.2.2.27

Application

Application Comment Organism
molecular biology UDG forms an apyrimidinic site, where an uracil base is removed, and facilitates the reactions of various DNA-repairing enzymes, such as AP endonuclease, DNA polymerase, and DNA ligase, it is thus useful in applications to remove uracil-DNA. Use of UDG to control carry-over contamination in PCR Bacillus sp. (in: Bacteria)

Cloned(Commentary)

Cloned (Comment) Organism
Bsp HJ171 UDG gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression of UDG containing an N-terminal intein cleavage site with an affinity chitin-binding tag under the control of a T7lac promoter, pTYB1 plasmid, in Escherichia coli strain BL21(DE3) Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activates optimal at 25 mM, recombinant UDG Bacillus sp. (in: Bacteria)
NaCl activates, optimal at 25 mM, recombinant UDG Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 x * 27000, recombinant detagged enzyme, SDS-PAGE, x * 27218, sequence calculation Bacillus sp. (in: Bacteria)
27218
-
 x * 27000, recombinant detagged enzyme, SDS-PAGE, x * 27218, sequence calculation Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) B6CM51
-
-
Bacillus sp. (in: Bacteria) HJ171 B6CM51
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant UDG from Escherichia coli strain BL21(DE3) by chitin affinity chromatogaphy to homogeneity, the tag is cleaved off by intein Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UDG specifically and selectively removes uracil bases from DNA, substrate specificity, overview Bacillus sp. (in: Bacteria) ?
-
 ?
additional information UDG specifically and selectively removes uracil bases from DNA, substrate specificity, overview Bacillus sp. (in: Bacteria) HJ171 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 27000, recombinant detagged enzyme, SDS-PAGE, x * 27218, sequence calculation Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
UDG
-
 Bacillus sp. (in: Bacteria)
UNG
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 25 recombinant UDG Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 the enzyme is heat-labile and cold-active Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the enzyme is heat-labile and cold-active Bacillus sp. (in: Bacteria)
30
-
 rapid decrease of enzyme activity above 30°C Bacillus sp. (in: Bacteria)
40
-
 half-life of the purified recombinant intein-tagged UDG is 131 s Bacillus sp. (in: Bacteria)
50
-
 half-life of the purified recombinant intein-tagged UDG is 45 s Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant UDG Bacillus sp. (in: Bacteria)