Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the interaction between Lhr and MtuNei2 may be crucial for recruiting MtuNei2 onto damaged DNA and stimulating the activity of MtuNei2 during DNA repair | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
genes Rv2464c and Rv3297, expression of His-tagged enzymes from a bicistronic vector in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | nei nth double mutants exhibit an elevated spontaneous mutation frequency. Spontaneous mutations observed in the double mutant are solely from C to T transitions due to the inability to repair oxidized cytosines, which can be efficiently prevented by MtuNei1 and MtuNei2 | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of MtuNei1 with diverse substrates, overview | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28500 | - |
x * 28500, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycobacterium tuberculosis | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
genes Rv2464c and Rv3297, encoding MtuNei1 and MtuNei2 | - |
Mycobacterium tuberculosis H37Rv | - |
genes Rv2464c and Rv3297, encoding MtuNei1 and MtuNei2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzymes from Escherichia coli by nickel affinity chromatograpyh | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea | Mycobacterium tuberculosis | ? | - |
? | |
additional information | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei1 excises thymine glycol and strongly prefers the 5R isomers. GC/MS analysis of products released by MtuNei1, overview. Substrates are oligonucleotides with 7,8-dihydro-8-oxoguanine, thymine glycol, 5,6-dihydrouracil, 5,6-dihydrothymine, 5-hydroxyuracil, 5-hydroxycytosine, uracil, and furan | Mycobacterium tuberculosis | ? | - |
? | |
additional information | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei1 excises thymine glycol and strongly prefers the 5R isomers. GC/MS analysis of products released by MtuNei1, overview. Substrates are oligonucleotides with 7,8-dihydro-8-oxoguanine, thymine glycol, 5,6-dihydrouracil, 5,6-dihydrothymine, 5-hydroxyuracil, 5-hydroxycytosine, uracil, and furan | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28500, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
endonuclease VIII | - |
Mycobacterium tuberculosis |
MtuNei1 | - |
Mycobacterium tuberculosis |
UDG | - |
Mycobacterium tuberculosis |
uracil DNA glycosylase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mycobacterium tuberculosis |