3.2.2.27: uracil-DNA glycosylase
This is an abbreviated version!
For detailed information about uracil-DNA glycosylase, go to the full flat file.
Word Map on EC 3.2.2.27
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3.2.2.27
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deamination
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cytosine
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glycosylases
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endonuclease
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thymine
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abasic
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misincorporation
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ung2
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uracil-containing
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duplex
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hypermutation
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dump
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deoxyuridine
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activation-induced
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excises
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mispairs
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base-excision
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dutpase
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8-oxoguanine
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apyrimidinic
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5-methylcytosine
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5-hydroxymethyluracil
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g-quadruplexes
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transversions
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ape1
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thymine-dna
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formamidopyrimidine
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5-hydroxyuracil
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short-patch
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xrcc1
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apurinic
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class-switching
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3,n4-ethenocytosine
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translesion
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post-replicative
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ap-endonuclease
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aid-induced
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apobec3g
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drug development
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molecular biology
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analysis
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medicine
- 3.2.2.27
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deamination
- cytosine
- glycosylases
- endonuclease
- thymine
-
abasic
-
misincorporation
- ung2
-
uracil-containing
- duplex
-
hypermutation
- dump
- deoxyuridine
-
activation-induced
-
excises
- mispairs
-
base-excision
- dutpase
- 8-oxoguanine
-
apyrimidinic
- 5-methylcytosine
- 5-hydroxymethyluracil
-
g-quadruplexes
-
transversions
- ape1
-
thymine-dna
- formamidopyrimidine
- 5-hydroxyuracil
-
short-patch
- xrcc1
-
apurinic
-
class-switching
- 3,n4-ethenocytosine
-
translesion
-
post-replicative
- ap-endonuclease
-
aid-induced
- apobec3g
- drug development
- molecular biology
- analysis
- medicine
Reaction
Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil =
Synonyms
5-formyluracil-DNA glycosylase, AFUDG, Afung protein, Blr0248, DNA N-glycosylase, dr0689, DR_0968, endonuclease VIII, family 1 uracil N-glycosylase, family 4 UDGa, family 4 uracil-DNA glycosylase, HMUDG, mismatch-specific uracil-DNA glycosylase, MjUDG, More, MtuNei1, MUG, Pa-UDG, PF1385, PfuUDG, single-strand selective monofunctional uracil-DNA glycosylase, single-strand selective uracil DNA glycosylase, single-strand-selective mono-functional uracil-DNA glycosylase, single-strand-selective monofunctional uracil DNA glycosylase, single-strand-selective monofunctional uracil-DNA glycosylase, single-stranded monofunctional uracil DNA N-glycosylase, SMUG1, SMUG1-like glycosylase, STK_22380, Ta0477, TD12-encoded protein, Thd1p, Thp1p, TMUDG, type 1 UDG, UDG, UDG1, UDGa, UdgB, UDGIV, UDGV, UL114, UL2, UNG, UNG-1, Ung-type uracil glycosylase, UNG1, UNG2, Unga, uracil DNA glycosylase, uracil DNA glycosylase 2, uracil DNA-glycosylase, uracil DNA-glycosylase 2, uracil-DNA glycosylase, uracil-DNA glycosylase 2, uracil-DNA glycosylase a, uracil-DNA N-glycosylase, uracil-N-glycosylase 2
ECTree
3.2.2.27 uracil-DNA glycosylaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.2.2.27 - uracil-DNA glycosylase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.0046
5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hSMUG1
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0.0027
5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hSMUG1
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0.0038
5-hydroxymethyluracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hSMUG1
-
0.00017
double stranded DNA containing G-U mismatch
mutant enzyme E41Q/G42D, at pH 7.6 and 60°C
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0.00074
double stranded DNA containing G-U mismatch
mutant enzyme N89A, at pH 7.6 and 60°C
-
0.00076
double stranded DNA containing G-U mismatch
mutant enzyme H155S, at pH 7.6 and 60°C
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0.00097
double stranded DNA containing G-U mismatch
wild type enzyme, at pH 7.6 and 60°C
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0.0004
uracil-containing calf thymus DNA
-
mutant enzyme G183D/R302K, at pH 7.5 and 37°C
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0.0005
uracil-containing calf thymus DNA
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wild type enzyme, at pH 7.5 and 15°C
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0.0006
uracil-containing calf thymus DNA
-
mutant enzyme G183D/R302K, at pH 7.5 and 22°C
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0.00074
uracil-containing calf thymus DNA
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mutant enzyme G183D, at pH 7.5 and 22°C
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0.0008
uracil-containing calf thymus DNA
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wild type enzyme, at pH 7.5 and 37°C
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0.0008
uracil-containing calf thymus DNA
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wild type enzyme, at pH 7.5 and 22°C
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0.00083
uracil-containing calf thymus DNA
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mutant enzyme G183D, at pH 7.5 and 37°C
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0.001
uracil-containing calf thymus DNA
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mutant enzyme G183D/R302K, at pH 7.5 and 15°C
-
0.00113
uracil-containing calf thymus DNA
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mutant enzyme G183D, at pH 7.5 and 15°C
-
0.00157
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 37°C
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0.0021
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 37°C
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0.0022
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 15°C
-
0.00226
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 15°C
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0.00238
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 15°C
-
0.0024
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 22°C
-
0.00265
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 37°C
-
0.00276
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 22°C
-
0.00277
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 22°C
-
0.000054
uracil-containing double-stranded DNA
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pH 7.5, 52°C, mutant enzyme K100A
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0.00007
uracil-containing double-stranded DNA
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pH 7.5, 52°C, wild-type enzyme
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0.000008
uracil-containing single-stranded DNA
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pH 7.5, 52°C, mutant enzyme K100A
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0.000011
uracil-containing single-stranded DNA
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pH 7.5, 52°C, wild-type enzyme
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0.000015
uracil-mismatched double-stranded DNA
pH 7.0, 37°C, excision of a single uracil residue from PS-G, recombinant enzyme
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0.00003
uracil-mismatched double-stranded DNA
pH 7.0, 37°C, excision of a single uracil residue from U-G, recombinant enzyme
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0.0007
uracil-mismatched double-stranded DNA
pH 8.0, 37°C, recombinant enzyme
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0.001
uracil-mismatched double-stranded DNA
pH 7.5, 37°C, excision of a single uracil residue from U-A, recombinant enzyme
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0.001
uracil-mismatched double-stranded DNA
pH 7.5, 37°C, excision of a single uracil residue from U-G, recombinant enzyme
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0.0014
uracil-mismatched double-stranded DNA
pH 7.5, 37°C, excision of a single uracil residue from U-T, recombinant enzyme
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0.0016
uracil-mismatched double-stranded DNA
pH 7.5, 37°C, excision of a single uracil residue from U-C, recombinant enzyme
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0.0017
uracil-mismatched double-stranded DNA
pH 7.5, 37°C, excision of a single uracil residue from PS-U, recombinant enzyme
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0.003
uracil-mismatched double-stranded DNA
pH 7.5, 80°C, cell extract (wild-type enzyme)
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0.0052
uracil-mismatched double-stranded DNA
pH 8.0, 37°C, recombinant wild-type enzyme
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0.0087
uracil-mismatched double-stranded DNA
pH 7.5, 80°C, recombinant enzyme
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0.009
uracil-mismatched double-stranded DNA
pH 8.0, 37°C, recombinant mutant A205S
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0.148
uracil-mismatched double-stranded DNA
pH 8.0, 37°C, recombinant mutant H200Q
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0.256
uracil-mismatched double-stranded DNA
pH 8.0, 37°C, recombinant mutant D77N
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0.0007
uracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hUNG2
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0.004
uracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
0.0000022
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 37°C, wild-type enzyme
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0.0004
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hUNG2
-
0.0013
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
0.0005
uracil-mismatched single-stranded DNA
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wild type enzyme, at pH 7.5 and 15°C
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0.0008
uracil-mismatched single-stranded DNA
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wild type enzyme, at pH 7.4 and 37°C
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0.001
uracil-mismatched single-stranded DNA
Q7WYV4
pH 7.5, 37°C, family 4 UDG
-
0.00157
uracil-mismatched single-stranded DNA
mutant enzyme G183G, at pH 7.4 and 37°C
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0.0017
uracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hSMUG1
-
0.0021
uracil-mismatched single-stranded DNA
wild type enzyme, at pH 7.4 and 37°C
-
0.0022
uracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hUNG2
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0.0022
uracil-mismatched single-stranded DNA
wild type enzyme, at pH 7.5 and 15°C
-
0.00265
uracil-mismatched single-stranded DNA
mutant enzyme G183G/K302R, at pH 7.4 and 37°C
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0.004
uracil-mismatched single-stranded DNA
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mutant enzyme G183D/R302K, at pH 7.4 and 37°C
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0.0083
uracil-mismatched single-stranded DNA
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mutant enzyme G183D, at pH 7.4 and 37°C
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additional information
additional information
kinetics of mutant enzymes, overview
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additional information
additional information
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kinetics of mutant enzymes, overview
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additional information
additional information
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steady-state kinetics of wild-type and mutant enzymes
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additional information
additional information
SMUG1 single-turnover kinetics using oligonucleotide substrates containing purine and pyrimidine analogs
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additional information
additional information
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SMUG1 single-turnover kinetics using oligonucleotide substrates containing purine and pyrimidine analogs
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additional information
additional information
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steady-state kinetics with several oligonucleitide substrate possessing U-A or U-G mispairs, overview
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additional information
additional information
substrate specificity, kinetic analysis
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additional information
additional information
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substrate specificity, kinetic analysis
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additional information
additional information
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kinetic parameters for the opposite-base dependent excision of 5-formyluracil from DNA by hSMUG1
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additional information
additional information
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kinetics of MtuNei1 with diverse substrates, overview
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additional information
additional information
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thermodynamics of UNG interactions with undamaged and damaged, e.g. 2'-deoxypseudouridine, substrates
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additional information
additional information
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thermodynamics of UNG interactions with undamaged and damaged, e.g. 2'-deoxypseudouridine, substrates
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additional information
additional information
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significantly different Km-values for the two substrates, with values of 70 nM for double-stranded DNA and 11 nM for single-stranded DNA
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