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Literature summary for 3.2.2.27 extracted from

  • Boorstein, R.J.; Cumming, A.; Marenstein, D.R.; Chan, M.K.; Ma, Y.; Neubert, T.A.; Brown, S.M.; Teebor, G.W.
    Definitive identification of mammalian 5-hydroxymethyluracil DNA N-glycosylase activity as SMUG1 (2001), J. Biol. Chem., 276, 41991-41997.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression as GST-tagged enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil-mismatched single-stranded DNA + H2O Homo sapiens
-
uracil + single-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O Bos taurus
-
uracil + single-stranded DNA with abasic site
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus Q59T47
-
-
Homo sapiens Q53HV7
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from thymus by ammonium sulfate fractionation, ion exchange chromatography, heparin affinity chromatography, and DNA affinity chromatography Bos taurus
recombinant GST-tagged enzyme about 500fold, the tag is removed Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
renaturation of the enzyme from gel slices after SDS-PAGE, method, overview Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxyuracil-mismatched single-stranded DNA + H2O DNA of Bacillus subtilis phage SPO1 Bos taurus 5-hydroxyuracil + single-stranded DNA with abasic site
-
?
additional information substrate specificity of SMUG, activity of hSMUG1 against uracil containing single- and double-stranded DNA containing matched and mismatched uracil, overview Homo sapiens ?
-
?
uracil-mismatched single-stranded DNA + H2O
-
Homo sapiens uracil + single-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O
-
Bos taurus uracil + single-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O single-stranded 26-mer uracil-containing 2'-deoxyribose oligonucleotide Homo sapiens uracil + single-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O single-stranded 26-mer uracil-containing 2'-deoxyribose oligonucleotide Bos taurus uracil + single-stranded DNA with abasic site
-
?

Subunits

Subunits Comment Organism
? x * 30000-34000, SDS-PAGE Homo sapiens
? x * 30000-34000, SDS-PAGE Bos taurus
More peptide mapping Bos taurus

Synonyms

Synonyms Comment Organism
DNA N-glycosylase
-
Homo sapiens
DNA N-glycosylase
-
Bos taurus
HMUDG
-
Bos taurus
single-stranded monofunctional uracil DNA N-glycosylase
-
Homo sapiens
single-stranded monofunctional uracil DNA N-glycosylase
-
Bos taurus
SMUG1
-
Homo sapiens
SMUG1
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens
7.5
-
assay at Bos taurus