Cloned (Comment) | Organism |
---|---|
expression as GST-tagged enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched single-stranded DNA + H2O | Homo sapiens | - |
uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | Bos taurus | - |
uracil + single-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q59T47 | - |
- |
Homo sapiens | Q53HV7 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from thymus by ammonium sulfate fractionation, ion exchange chromatography, heparin affinity chromatography, and DNA affinity chromatography | Bos taurus |
recombinant GST-tagged enzyme about 500fold, the tag is removed | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
renaturation of the enzyme from gel slices after SDS-PAGE, method, overview | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxyuracil-mismatched single-stranded DNA + H2O | DNA of Bacillus subtilis phage SPO1 | Bos taurus | 5-hydroxyuracil + single-stranded DNA with abasic site | - |
? | |
additional information | substrate specificity of SMUG, activity of hSMUG1 against uracil containing single- and double-stranded DNA containing matched and mismatched uracil, overview | Homo sapiens | ? | - |
? | |
uracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | - |
Bos taurus | uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | single-stranded 26-mer uracil-containing 2'-deoxyribose oligonucleotide | Homo sapiens | uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | single-stranded 26-mer uracil-containing 2'-deoxyribose oligonucleotide | Bos taurus | uracil + single-stranded DNA with abasic site | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000-34000, SDS-PAGE | Homo sapiens |
? | x * 30000-34000, SDS-PAGE | Bos taurus |
More | peptide mapping | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
DNA N-glycosylase | - |
Homo sapiens |
DNA N-glycosylase | - |
Bos taurus |
HMUDG | - |
Bos taurus |
single-stranded monofunctional uracil DNA N-glycosylase | - |
Homo sapiens |
single-stranded monofunctional uracil DNA N-glycosylase | - |
Bos taurus |
SMUG1 | - |
Homo sapiens |
SMUG1 | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Bos taurus |