3.2.1.B28: Pyrococcus furiosus beta-glycosidase
This is an abbreviated version!
For detailed information about Pyrococcus furiosus beta-glycosidase, go to the full flat file.
Reaction
The enzyme produces mannose and glucose mainly from mannooligosaccharides and cellobiose by hydrolyzing them from the reducing end. It can not hydrolyze alpha-form substrates and bulk polymers like mannan and starch =
Synonyms
beta-glycosidase, Bgl, BGLPf, CelB, PFTG
ECTree
3.2.1.B28 Pyrococcus furiosus beta-glycosidaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.2.1.B28 - Pyrococcus furiosus beta-glycosidase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
5.3
2-nitrophenyl beta-D-galactoside
release of 2-nitrophenol, pH 7.5, 80°C
46
pentyl beta-D-glucopyranoside
pH 5.0, 80°C, 6% water, 94% hexanol
additional information
additional information
mutants enzymes R77Q and R77Q/N206D show a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside
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0.89
2-nitrophenyl beta-D-galactopyranoside
mutant F426Y, pH 5.0, 90°C
2.3
2-nitrophenyl beta-D-galactopyranoside
wild-type, pH 5.0, 90°C
4.8
2-nitrophenyl beta-D-galactopyranoside
mutant M424K, pH 5.0, 90°C
40
2-nitrophenyl beta-D-galactopyranoside
mutant E417S/M424K/F426Y, pH 5.0, 90°C
42
2-nitrophenyl beta-D-galactopyranoside
mutant E417S, pH 5.0, 90°C
16
2-nitrophenyl beta-D-galactopyranoside-6-phosphate
mutant E417S/M424K/F426Y, pH 5.0, 90°C
22
2-nitrophenyl beta-D-galactopyranoside-6-phosphate
mutant E417S, pH 5.0, 90°C
31
2-nitrophenyl beta-D-galactopyranoside-6-phosphate
wild-type, pH 5.0, 90°C
38
2-nitrophenyl beta-D-galactopyranoside-6-phosphate
mutant F426Y, pH 5.0, 90°C
0.1
2-nitrophenyl beta-D-glucopyranoside
pH 5.0, 95°C, mutant enzyme Q77R/Q150W
0.22
2-nitrophenyl beta-D-glucopyranoside
wild-type, pH 6.5, 65°C, presence of 2 M formate
0.3
2-nitrophenyl beta-D-glucopyranoside
pH 5.0, 95°C, mutant enzyme Q150W
0.51
2-nitrophenyl beta-D-glucopyranoside
mutant M424K, pH 5.0, 90°C
0.57
2-nitrophenyl beta-D-glucopyranoside
mutant F426Y, pH 5.0, 90°C
1.3
2-nitrophenyl beta-D-glucopyranoside
mutant E372A, pH 5.0, 65°C
1.49
2-nitrophenyl beta-D-glucopyranoside
mutant E372A, pH 6.5, 65°C, presence of 2 M formate
2.1
2-nitrophenyl beta-D-glucopyranoside
mutant E372A, pH 4.0, 65°C
4.3
2-nitrophenyl beta-D-glucopyranoside
mutant E372A, pH 3.0, 65°C
29
2-nitrophenyl beta-D-glucopyranoside
mutant E417S, pH 5.0, 90°C
59
2-nitrophenyl beta-D-glucopyranoside
mutant E417S/M424K/F426Y, pH 5.0, 90°C
0.7
4-nitrophenyl beta-D-galactopyranoside
wild-type, pH 4.8, 20°C
1.2
4-nitrophenyl beta-D-galactopyranoside
mutant N4515S, pH 4.8, 20°C
1.3
4-nitrophenyl beta-D-galactopyranoside
mutant T371A, pH 4.8, 20°C
3.4
4-nitrophenyl beta-D-galactopyranoside
mutant M424V, pH 4.8, 20°C
4.5
4-nitrophenyl beta-D-galactopyranoside
mutant A419T, pH 4.8, 20°C
5
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, wild-type enzyme
8.5
4-nitrophenyl beta-D-galactopyranoside
mutant N4515S, pH 4.8, 90°C
15.7
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme N206D
21.7
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme R77Q
22
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme R77Q/N206D
0.02
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 95°C, mutant enzyme Q77R/Q150W
0.05
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 95°C, mutant enzyme Q150W
0.19
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, wild-type enzyme
0.39
4-nitrophenyl beta-D-glucopyranoside
90°C, pH not specified in the publication, enzyme from Pyrococcus furiosus
0.39
4-nitrophenyl beta-D-glucopyranoside
pH 6.5, temperature not specified in the publication, recombinant enzyme
0.41
4-nitrophenyl beta-D-glucopyranoside
90°C, pH not specified in the publication, enzyme expressed in Escherichia coli
0.41
4-nitrophenyl beta-D-glucopyranoside
pH 6.5, temperature not specified in the publication, enzyme purified from Pyrococcus furiosus
0.57
4-nitrophenyl beta-D-glucopyranoside
mutant T371A, pH 4.8, 20°C
0.92
4-nitrophenyl beta-D-glucopyranoside
mutant M424V, pH 4.8, 20°C
1.1
4-nitrophenyl beta-D-glucopyranoside
mutant N4515S, pH 4.8, 20°C
1.3
4-nitrophenyl beta-D-glucopyranoside
mutant A419T, pH 4.8, 20°C
1.4
4-nitrophenyl beta-D-glucopyranoside
mutant N4515S, pH 4.8, 90°C
4.6
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme N206D
13.8
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme R77Q
13.9
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme R77Q/N206D
21
4-nitrophenyl beta-D-glucoside
pH 5.0, 95°C, mutant enzyme F426Y
27
4-nitrophenyl beta-D-glucoside
pH 5.0, 95°C, mutant enzyme M424K/F426Y
39
4-nitrophenyl beta-D-glucoside
pH 5.0, 95°C, mutant enzyme M424K
0.03
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 95°C, mutant enzyme Q77R/Q150W
0.05
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 95°C, mutant enzyme Q150W
0.1
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme R77Q/N206D, below 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside
0.15
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme R77Q, below 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside
0.63
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme N206D
0.64
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme R77Q, above 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside
1.3
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, wild-type enzyme
1.5
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme R77Q/N206D, above 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside
