3.2.1.B28: Pyrococcus furiosus beta-glycosidase

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For detailed information about Pyrococcus furiosus beta-glycosidase, go to the full flat file.

Reaction

The enzyme produces mannose and glucose mainly from mannooligosaccharides and cellobiose by hydrolyzing them from the reducing end. It can not hydrolyze alpha-form substrates and bulk polymers like mannan and starch =

Synonyms

beta-glycosidase, Bgl, BGLPf, CelB, PFTG

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.B28 Pyrococcus furiosus beta-glycosidase

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Results	in table
1	Activating Compound
2	AA Sequence
17	Application
17	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
5	General Stability
18	Inhibitors
89	kcat/KM [mM/s]
9	Ki Value [mM]
127	KM Value [mM]
1	Localization
2	Metals/Ions
13	Molecular Weight [Da]
1	Organic Solvent Stability
38	Organism
17	pH Optimum
4	pH Range
1	pH Stability
1	pI Value
26	Protein Variants
10	Purification (Commentary)
1	Reaction
38	Reference
40	Specific Activity [micromol/min/mg]
103	Substrates and Products (Substrate)
13	Subunits
20	Synonyms
15	Temperature Optimum [°C]
4	Temperature Range [°C]
45	Temperature Stability [°C]
109	Turnover Number [1/s]

Expression

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Expression on EC 3.2.1.B28 - Pyrococcus furiosus beta-glycosidase

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during growth of Pyrococcus furiosus on either cellobiose or laminarin, the activities of both beta-glucosidase and endoglucanase are increased at least fivefold compared with levels during growth on maltose or pyruvate, due to an enhanced transcription of both the celB gene and the lamA operon in the presence of these glucose-containing substrates

Pyrococcus furiosus

Q51723

736342

gene locus includes the celB gene, and a divergently orientated gene cluster, adhA-adhB-lamA, which codes for two alcohol dehydrogenases and an extracellular beta-1,3-endoglucanase. The in vivo and in vitro transcription initiation sites of both the celB gene and the lamA operon lie 25 nucleotides downstream of conserved TATA box motifs

Pyrococcus furiosus

Q51723

736342