Literature summary for 3.2.1.B28 extracted from

Hansson, T.; Adlercreutz, P.
The temperature influences the ratio of glucosidase and galactosidase activities of beta-glycosidases (2002), Biotechnol. Lett., 24, 1465-1471.

No PubMed abstract available

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.3	-	4-nitrophenyl beta-D-galactoside	pH 5.0, 90°C	Pyrococcus furiosus
2.6	-	4-nitrophenyl beta-D-glucoside	pH 5.0, 90°C	Pyrococcus furiosus
4.6	-	4-nitrophenyl beta-D-galactoside	pH 5.0, 70°C	Pyrococcus furiosus
7.2	-	4-nitrophenyl beta-D-galactoside	pH 5.0, 50°C	Pyrococcus furiosus
9.1	-	4-nitrophenyl beta-D-glucoside	pH 5.0, 70°C	Pyrococcus furiosus
20	-	4-nitrophenyl beta-D-glucoside	pH 5.0, 50°C	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	Q51723	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-galactoside + H2O	at 80°C 61% of the activity with 4-nitrophenyl beta-D-glucoside	Pyrococcus furiosus	4-nitrophenol + D-galactose	-	?
4-nitrophenyl beta-D-glucoside + H2O	-	Pyrococcus furiosus	4-nitrophenol + D-glucose	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	CelB shows almost constant selcetivity for the glycon part of a donor substrate as the temperature increases. The specificity constant (Vmax/Km) ratio of phenyl-beta-glucoside and phenyl-beta-galactoside substrate decreases only slightly from 2.6 to 2.1 between 50 and 90°C	Pyrococcus furiosus

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Release 2023.1 (January 2023)