3.2.1.B28: Pyrococcus furiosus beta-glycosidase
This is an abbreviated version!
For detailed information about Pyrococcus furiosus beta-glycosidase, go to the full flat file.
Reaction
The enzyme produces mannose and glucose mainly from mannooligosaccharides and cellobiose by hydrolyzing them from the reducing end. It can not hydrolyze alpha-form substrates and bulk polymers like mannan and starch =
Synonyms
beta-glycosidase, Bgl, BGLPf, CelB, PFTG
ECTree
3.2.1.B28 Pyrococcus furiosus beta-glycosidaseAdvanced search results
results (
results (KCat KM Value
KCat KM Value on EC 3.2.1.B28 - Pyrococcus furiosus beta-glycosidase
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kcat/KM VALUE [1/mMs-1] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
1900
2-nitrophenyl beta-D-galactoside
release of 2-nitrophenol, pH 7.5, 80°C
additional information
additional information
mutants enzymes R77Q and R77Q/N206D show a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside
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4.4
2-nitrophenyl beta-D-galactopyranoside
mutant E417S/M424K/F426Y, pH 5.0, 90°C
25.3
2-nitrophenyl beta-D-galactopyranoside
mutant E417S, pH 5.0, 90°C
396
2-nitrophenyl beta-D-galactopyranoside
mutant M424K, pH 5.0, 90°C
899
2-nitrophenyl beta-D-galactopyranoside
wild-type, pH 5.0, 90°C
1000
2-nitrophenyl beta-D-galactopyranoside
mutant F426Y, pH 5.0, 90°C
0.7
2-nitrophenyl beta-D-galactopyranoside-6-phosphate
mutant F426Y, pH 5.0, 90°C
1.6
2-nitrophenyl beta-D-galactopyranoside-6-phosphate
wild-type, pH 5.0, 90°C
4.2
2-nitrophenyl beta-D-galactopyranoside-6-phosphate
mutant E417S/M424K/F426Y, pH 5.0, 90°C
7.7
2-nitrophenyl beta-D-galactopyranoside-6-phosphate
mutant E417S, pH 5.0, 90°C
0.3
2-nitrophenyl beta-D-glucopyranoside
mutant E372A, pH 5.0, 65°C
2.17
2-nitrophenyl beta-D-glucopyranoside
pH 5.0, 95°C, mutant enzyme Q77R/Q150W
2.26
2-nitrophenyl beta-D-glucopyranoside
pH 5.0, 95°C, mutant enzyme Q150W
2.6
2-nitrophenyl beta-D-glucopyranoside
mutant E372A, pH 6.5, 65°C, presence of 2 M formate
3.1
2-nitrophenyl beta-D-glucopyranoside
mutant E372A, pH 4.0, 65°C
10.9
2-nitrophenyl beta-D-glucopyranoside
mutant E372A, pH 3.0, 65°C
13
2-nitrophenyl beta-D-glucopyranoside
mutant E417S/M424K/F426Y, pH 5.0, 90°C
113
2-nitrophenyl beta-D-glucopyranoside
mutant E417S, pH 5.0, 90°C
1484
2-nitrophenyl beta-D-glucopyranoside
mutant F426Y, pH 5.0, 90°C
1510
2-nitrophenyl beta-D-glucopyranoside
mutant M424K, pH 5.0, 90°C
5350
2-nitrophenyl beta-D-glucopyranoside
wild-type, pH 6.5, 65°C, presence of 2 M formate
1.3
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme R77Q/N206D
3.1
4-nitrophenyl beta-D-galactopyranoside
mutant A419T, pH 4.8, 20°C
3.2
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme R77Q
3.4
4-nitrophenyl beta-D-galactopyranoside
mutant M424V, pH 4.8, 20°C
3.5
4-nitrophenyl beta-D-galactopyranoside
mutant N4515S, pH 4.8, 20°C
8.2
4-nitrophenyl beta-D-galactopyranoside
mutant T371A, pH 4.8, 20°C
11.5
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme N206D
120
4-nitrophenyl beta-D-galactopyranoside
mutant N4515S, pH 4.8, 90°C
480
4-nitrophenyl beta-D-galactopyranoside
wild-type, pH 4.8, 90°C
561
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, wild-type enzyme
2.44
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 95°C, mutant enzyme Q150W
2.95
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 95°C, mutant enzyme Q77R/Q150W
12.3
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme R77Q/N206D
14.9
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme R77Q
24.9
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme N206D
31
4-nitrophenyl beta-D-glucopyranoside
mutant A419T, pH 4.8, 20°C
43
4-nitrophenyl beta-D-glucopyranoside
mutant M424V, pH 4.8, 20°C
43
4-nitrophenyl beta-D-glucopyranoside
mutant N4515S, pH 4.8, 20°C
45
4-nitrophenyl beta-D-glucopyranoside
mutant T371A, pH 4.8, 20°C
1000
4-nitrophenyl beta-D-glucopyranoside
mutant N4515S, pH 4.8, 90°C
7337
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, wild-type enzyme
1.3
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme R77Q/N206D, above 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside
1.9
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme R77Q, above 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside
2.83
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 95°C, mutant enzyme Q150W
3.1
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme N206D
3.63
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 95°C, mutant enzyme Q77R/Q150W
5.7
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme R77Q, below 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside
10.2
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme R77Q/N206D, below 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside
49.8
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, wild-type enzyme
