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Literature summary for 3.2.1.B28 extracted from
- Expression and characterization of an extremely thermostable beta-glycosidase (mannosidase) from the hyperthermophilic archaeon Pyrococcus furiosus DSM3638 (2011), N. Biotechnol., 28, 639-648.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the hyperthermostable beta-glycosidase may be useful for food and pharmaceutical applications | Pyrococcus furiosus |
| pharmacology | the hyperthermostable beta-glycosidase may be useful for food and pharmaceutical applications | Pyrococcus furiosus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
lactose | pH 5.0, 100°C | Pyrococcus furiosus |  |
| 0.07 | - |
cellobiose | pH 5.0, 100°C | Pyrococcus furiosus | |
| 0.09 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
| 0.09 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
| 0.12 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56326 | - |
4 * 56326, calculated from sequence | Pyrococcus furiosus |
| 57000 | - |
4 * 57000, SDS-PAGE | Pyrococcus furiosus |
| 230000 | - |
non-denaturing PAGE | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q8U3U9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | 37% of the activity compared to 4-nitrophenyl-beta-D-mannopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | 114% of the activity compared to 4-nitrophenyl-beta-D-mannopyranoside | Pyrococcus furiosus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + beta-D-mannopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | 37% of the activity compared to 4-nitrophenyl-beta-D-mannopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| cellobiose + H2O | - |
Pyrococcus furiosus | 2 beta-D-glucose | - |
? | |
| lactose + H2O | - |
Pyrococcus furiosus | D-glucose + D-galactose | - |
? | |
| mannooligosaccharide + H2O | hydrolysis of mannose from the reducing end | Pyrococcus furiosus | ? | - |
? | |
| additional information | the enzyme shows transglycosylation and transgalactosylation activities toward cellobiose, lactose and mannooligosaccharides that could produce galactooligosaccharides and maltooligosaccharides. It can not hydrolyze alpha-form substrates and bulk polymers like mannan and starch | Pyrococcus furiosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 57000, SDS-PAGE | Pyrococcus furiosus |
| tetramer | 4 * 56326, calculated from sequence | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PFTG | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 110 | 70°C: about 50% of maximal activity, 110°C: about 45% of maximal activity | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
2 h, stable | Pyrococcus furiosus |
| 90 | - |
half-life: 398 min | Pyrococcus furiosus |
| 95 | - |
half-life: 114 min | Pyrococcus furiosus |
| 100 | - |
1 h, 73% loss of activity | Pyrococcus furiosus |
| 110 | - |
thermal transition midpoint | Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
lactose | pH 5.0, 100°C | Pyrococcus furiosus | |
| 0.16 | - |
cellobiose | pH 5.0, 100°C | Pyrococcus furiosus | |
| 0.23 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
| 0.26 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
| 0.3 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
sodium citrate buffer | Pyrococcus furiosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6 | pH 4.5: about 85% of maximal activity, pH 6.0: about 60% of maximal activity | Pyrococcus furiosus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.82 | - |
lactose | pH 5.0, 100°C | Pyrococcus furiosus | |
| 1.89 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
| 2.28 | - |
cellobiose | pH 5.0, 100°C | Pyrococcus furiosus | |
| 2.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
| 3.02 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
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