3.2.1.B28: Pyrococcus furiosus beta-glycosidase

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For detailed information about Pyrococcus furiosus beta-glycosidase, go to the full flat file.

Reaction

The enzyme produces mannose and glucose mainly from mannooligosaccharides and cellobiose by hydrolyzing them from the reducing end. It can not hydrolyze alpha-form substrates and bulk polymers like mannan and starch =

Synonyms

beta-glycosidase, Bgl, BGLPf, CelB, PFTG

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.B28 Pyrococcus furiosus beta-glycosidase

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Results	in table
1	Activating Compound
2	AA Sequence
17	Application
17	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
5	General Stability
18	Inhibitors
89	kcat/KM [mM/s]
9	Ki Value [mM]
127	KM Value [mM]
1	Localization
2	Metals/Ions
13	Molecular Weight [Da]
1	Organic Solvent Stability
38	Organism
17	pH Optimum
4	pH Range
1	pH Stability
1	pI Value
26	Protein Variants
10	Purification (Commentary)
1	Reaction
38	Reference
40	Specific Activity [micromol/min/mg]
103	Substrates and Products (Substrate)
13	Subunits
20	Synonyms
15	Temperature Optimum [°C]
4	Temperature Range [°C]
45	Temperature Stability [°C]
109	Turnover Number [1/s]

Temperature Optimum

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Temperature Optimum on EC 3.2.1.B28 - Pyrococcus furiosus beta-glycosidase

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100

102 - 105

104

105

recombinant protein

assay at

assay at

assay at

additional information

Pyrococcus furiosus

Q51723

CelB shows almost constant selcetivity for the glycon part of a donor substrate as the temperature increases. The specificity constant (Vmax/Km) ratio of phenyl-beta-glucoside and phenyl-beta-galactoside substrate decreases only slightly from 2.6 to 2.1 between 50 and 90°C

724633