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3.2.1.B28: Pyrococcus furiosus beta-glycosidase

This is an abbreviated version!
For detailed information about Pyrococcus furiosus beta-glycosidase, go to the full flat file.

Reaction

The enzyme produces mannose and glucose mainly from mannooligosaccharides and cellobiose by hydrolyzing them from the reducing end. It can not hydrolyze alpha-form substrates and bulk polymers like mannan and starch =

Synonyms

beta-glycosidase, Bgl, BGLPf, CelB, PFTG

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.B28 Pyrococcus furiosus beta-glycosidase

Inhibitors

Inhibitors on EC 3.2.1.B28 - Pyrococcus furiosus beta-glycosidase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol
1,3-dimethylimidazolium methylsulfate
the enzyme retains full catalytic efficiency for lactose hydrolysis at 80°C in a 50% (v/v) solution of the ionic liquid 1,3-dimethylimidazoliummethylsulfate in sodium citrate buffer, pH 5.5. It is inactive but not irreversibly denatured at 70% (v/v) ionic liquid
1-methyl-1-butanol
10% (v/v), about% inhibition
2-propanol
10% (v/v), about% inhibition
acetone
10% (v/v), about% inhibition
D-galactose
measured with lactose as the substrate
D-glucose
DMSO
10% (v/v), about% inhibition
ethanol
10% (v/v), about 50% inhibition
formate
10% (v/v), about% inhibition
Isopropanol
10% (v/v), about% inhibition
methanol
10% (v/v), about% inhibition
additional information
not inhibited by EDTA
-