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Literature summary for 3.2.1.B28 extracted from

  • Park, S.; Alli, I.; Park, K.; Oh, B.; Lee, B.
    Mutation studies in the active site of beta-glycosidase from Pyrococcus furiosus DSM 3638 (2013), Protein Pept. Lett., 20, 107-114.
    View publication on PubMed
Search for more literature for 3.2.1.B28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, mutant enzymes Q150W and Q77R/Q150W Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
Q150W the mutant enzyme has more affinity (Km) towards each substrate but shows lower turnover number (kcat) compared to the wild-type enzyme Pyrococcus furiosus
Q77R/Q150W the mutant enzyme has more affinity (Km) towards each substrate but shows lower turnover number (kcat) compared to the wild-type enzyme Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 lactose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
0.02
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
0.03
-
 lactose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
0.03
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
0.04
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
0.05
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
0.05
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
0.1
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
0.3
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
0.38
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 x * 57000, SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U3U9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + beta-D-mannopyranose
-
 ?
cellobiose + H2O
-
 Pyrococcus furiosus 2 beta-D-glucose
-
 ?
lactose + H2O
-
 Pyrococcus furiosus D-glucose + D-galactose
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
PFTG
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
 assay at Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 lactose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
0.03
-
 lactose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
0.05
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
0.1
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
0.11
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
0.13
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
0.13
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
0.22
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
0.67
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
0.74
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 sodium citrate buffer, assay at Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.36
-
 lactose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
1.92
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
2.17
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
2.21
-
 lactose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
2.26
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
2.44
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
2.71
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
2.83
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
2.95
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.63
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus