3.2.1.B28: Pyrococcus furiosus beta-glycosidase
This is an abbreviated version!
For detailed information about Pyrococcus furiosus beta-glycosidase, go to the full flat file.
Reaction
The enzyme produces mannose and glucose mainly from mannooligosaccharides and cellobiose by hydrolyzing them from the reducing end. It can not hydrolyze alpha-form substrates and bulk polymers like mannan and starch =
Synonyms
beta-glycosidase, Bgl, BGLPf, CelB, PFTG
ECTree
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KI Value
KI Value on EC 3.2.1.B28 - Pyrococcus furiosus beta-glycosidase
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0.0000065 - 0.0098
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol
0.0000065
pH 5.0, 90°C, wild-type enzyme
0.0000093
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol
pH 5.0, 70°C, substrate: 4-nitrophenyl beta-D-galactopyranoside
0.000097
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol
pH 5.0, 90°C, mutant enzyme R77Q
0.0027
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol
pH 5.0, 90°C, mutant enzyme R77Q/N206D
0.0098
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol
pH 5.0, 90°C, mutant enzyme N206D
43
pH 5.0, 70°C, substrate: 4-nitrophenyl beta-D-galactopyranoside