3.2.1.B28: Pyrococcus furiosus beta-glycosidase
This is an abbreviated version!
For detailed information about Pyrococcus furiosus beta-glycosidase, go to the full flat file.
Reaction
The enzyme produces mannose and glucose mainly from mannooligosaccharides and cellobiose by hydrolyzing them from the reducing end. It can not hydrolyze alpha-form substrates and bulk polymers like mannan and starch =
Synonyms
beta-glycosidase, Bgl, BGLPf, CelB, PFTG
ECTree
3.2.1.B28 Pyrococcus furiosus beta-glycosidaseAdvanced search results
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results (Cloned
Cloned on EC 3.2.1.B28 - Pyrococcus furiosus beta-glycosidase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli, mutant enzymes Q150W and Q77R/Q150W
expression in in Lactobacillus plantarum NC8 and Lactobacillus casei as hosts. Both lactobacilli harboring the pSIP409-celB vector produce active CelB in batch bioreactor cultivations while the specific CelB activity of the cell free extract is about 44% higher with Lactobacillus plantarum than with Lactobacillus casei using 4-nitrophenyl beta-galactoside as the substrate. A fedbatch bioreactor cultivation of Lactobacillus plantarum NC8 pSIP409-celB results in a specific CelB activity of 2500 nkat 4-nitrophenyl beta-D-galactopyranoside/mg protein after 28 h. A repeated dosage of the inducer (sakacin P inducing peptide) does not increase the enzyme expression further
expression of wild-type enzyme and mutant enzymes in Escherichia coli
expression using a baculovirus expression vector system in silkworm, Bombyx mori
overexpressed in Escherichia coli, resulting in high-level (up to 20% of total protein) production of the enzyme
recombinant mutant that lacks the C-terminal 23 residues and includes six substitutive mutations R170A, R220A, Y227F, F447S, R448V and E449Kis expressed in Escherichia coli BL21
expression in Escherichia coli
