Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
N206N | about 300fold decrease in catalytic efficiency for 4-nitrophenyl-beta-D-glucospyranoside, about 50fold decrease in catalytic efficiency for 4-nitrophenyl-beta-D-galactoside | Pyrococcus furiosus |
R77Q | 2000fold decrease in catalytic efficiency for 4-nitrophenyl-beta-D-glucospyranoside, 175fold decrease in catalytic efficiency for 4-nitrophenyl-beta-D-galactoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus |
R77Q/N206D | about 600fold decrease in catalytic efficiency for 4-nitrophenyl-beta-D-glucospyranoside, about 430fold decrease in catalytic efficiency for 4-nitrophenyl-beta-D-galactoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol | - |
Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutants enzymes R77Q and R77Q/N206D show a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
0.1 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D, below 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
0.15 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q, below 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
0.19 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus furiosus | |
0.63 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme N206D | Pyrococcus furiosus | |
0.64 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q, above 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
1.3 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus furiosus | |
1.5 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D, above 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
4.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme N206D | Pyrococcus furiosus | |
5 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus furiosus | |
13.8 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme R77Q | Pyrococcus furiosus | |
13.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D | Pyrococcus furiosus | |
15.7 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme N206D | Pyrococcus furiosus | |
21.7 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme R77Q | Pyrococcus furiosus | |
22 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q51723 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | catalytic efficiency (kcat/Km) for 4-nitrophenyl beta-D-galactopyranoside is 7.6% compared to catalytic catalytic efficiency (kcat/Km) for 4-nitrophenyl beta-D-glucopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-mannopyranoside + H2O | catalytic efficiency (kcat/Km) for 4-nitrophenyl beta-D-mannopyranoside is 0.7% compared to catalytic catalytic efficiency (kcat/Km) for 4-nitrophenyl beta-D-glucopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-mannose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CelB | - |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
102 | - |
half-life: over 10 h | Pyrococcus furiosus |
106 | - |
melting temperature | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutants enzymes R77Q and R77Q/N206D show a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
0.85 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q, below 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
1.02 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D, below 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
1.22 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q, above 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
1.9 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D, above 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
1.97 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme N206D | Pyrococcus furiosus | |
29.2 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D | Pyrococcus furiosus | |
65.9 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus furiosus | |
69.2 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme R77Q | Pyrococcus furiosus | |
116 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme N206D | Pyrococcus furiosus | |
172 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D | Pyrococcus furiosus | |
180 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme N206D | Pyrococcus furiosus | |
207 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme R77Q | Pyrococcus furiosus | |
1140 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus furiosus | |
2827 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus furiosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000065 | - |
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol | pH 5.0, 90°C, wild-type enzyme | Pyrococcus furiosus | |
0.000097 | - |
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol | pH 5.0, 90°C, mutant enzyme R77Q | Pyrococcus furiosus | |
0.0027 | - |
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol | pH 5.0, 90°C, mutant enzyme R77Q/N206D | Pyrococcus furiosus | |
0.0098 | - |
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol | pH 5.0, 90°C, mutant enzyme N206D | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutants enzymes R77Q and R77Q/N206D show a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
1.3 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D | Pyrococcus furiosus | |
1.3 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D, above 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
1.9 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q, above 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
3.1 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme N206D | Pyrococcus furiosus | |
3.2 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme R77Q | Pyrococcus furiosus | |
5.7 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q, below 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
10.2 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D, below 1 mM 4-nitrophenyl beta-D-mannopyranoside. The mutant enzyme shows a biphasic behavior for the hydrolysis of 4-nitrophenyl beta-D-mannopyranoside with separate kinetic parameters above and below 1 mM 4-nitrophenyl beta-D-mannopyranoside | Pyrococcus furiosus | |
11.5 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, mutant enzyme N206D | Pyrococcus furiosus | |
12.3 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme R77Q/N206D | Pyrococcus furiosus | |
14.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme R77Q | Pyrococcus furiosus | |
24.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, mutant enzyme N206D | Pyrococcus furiosus | |
49.8 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus furiosus | |
561 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus furiosus | |
7337 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, wild-type enzyme | Pyrococcus furiosus |