Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and mutant enzymes in Escherichia coli | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
F426Y | mutant has lower activity than the wild-type enzyme, but provides a higher ratio of transglucosylation product to hydrolysis products compared to wild-type enzyme, 2.6fold increase. The mutant enzyme has higher selectivity over the wide range of temperatures tested | Pyrococcus furiosus |
M424K | mutant has lower activity than the wild-type enzyme, but provides a higher ratio of transglucosylation product to hydrolysis products compared to wild-type enzyme | Pyrococcus furiosus |
M424K/F426Y | mutant has lower activity than the wild-type enzyme, but provides a higher ratio of transglucosylation product to hydrolysis products compared to wild-type enzyme | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme F426Y | Pyrococcus furiosus | |
27 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme M424K/F426Y | Pyrococcus furiosus | |
39 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme M424K | Pyrococcus furiosus | |
49 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, wild-type enzyme | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54665 | - |
x * 54665, calculated from sequence | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q51723 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + beta-D-glucose | - |
? | |
additional information | the enzyme also shows transglucosylation activity | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54665, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
CelB | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme M424K/F426Y | Pyrococcus furiosus | |
0.9 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme F426Y | Pyrococcus furiosus | |
5 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, mutant enzyme M424K | Pyrococcus furiosus | |
7.1 | - |
4-nitrophenyl beta-D-glucoside | pH 5.0, 95°C, wild-type enzyme | Pyrococcus furiosus |