Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
overexpressed in Escherichia coli, resulting in high-level (up to 20% of total protein) production of the enzyme | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
E372D | 200-fold reduction in specific activity | Pyrococcus furiosus |
E372Q | 1000-fold reduction in specific activity | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
4-nitrophenyl beta-D-glucopyranoside | 90°C, pH not specified in the publication, enzyme from Pyrococcus furiosus | Pyrococcus furiosus | |
0.39 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, temperature not specified in the publication, recombinant enzyme | Pyrococcus furiosus | |
0.41 | - |
4-nitrophenyl beta-D-glucopyranoside | 90°C, pH not specified in the publication, enzyme expressed in Escherichia coli | Pyrococcus furiosus | |
0.41 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, temperature not specified in the publication, enzyme purified from Pyrococcus furiosus | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54580 | - |
x * 54580, calculated from sequence | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q51723 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54580, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
CelB | - |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
30 h, enzyme produced by Escherichia coli retains 80% of its activity, enzyme from Pyrococcus furiosus retains 90% of its activity | Pyrococcus furiosus |
100 | - |
30 h, enzyme purified from Pyrococcus furiosus and recombinant enzyme retains 80 to 90% of the initial activity | Pyrococcus furiosus |