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Literature summary for 3.2.1.B28 extracted from

  • Voorhorst, W.G.; Eggen, R.I.; Luesink, E.J.; de Vos, W.M.
    Characterization of the celB gene coding for beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus and its expression and site-directed mutation in Escherichia coli (1995), J. Bacteriol., 177, 7105-7111.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus
overexpressed in Escherichia coli, resulting in high-level (up to 20% of total protein) production of the enzyme Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
E372D 200-fold reduction in specific activity Pyrococcus furiosus
E372Q 1000-fold reduction in specific activity Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
4-nitrophenyl beta-D-glucopyranoside 90°C, pH not specified in the publication, enzyme from Pyrococcus furiosus Pyrococcus furiosus
0.39
-
4-nitrophenyl beta-D-glucopyranoside pH 6.5, temperature not specified in the publication, recombinant enzyme Pyrococcus furiosus
0.41
-
4-nitrophenyl beta-D-glucopyranoside 90°C, pH not specified in the publication, enzyme expressed in Escherichia coli Pyrococcus furiosus
0.41
-
4-nitrophenyl beta-D-glucopyranoside pH 6.5, temperature not specified in the publication, enzyme purified from Pyrococcus furiosus Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54580
-
x * 54580, calculated from sequence Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q51723
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Pyrococcus furiosus 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Pyrococcus furiosus 4-nitrophenol + beta-D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 54580, calculated from sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
CelB
-
Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
30 h, enzyme produced by Escherichia coli retains 80% of its activity, enzyme from Pyrococcus furiosus retains 90% of its activity Pyrococcus furiosus
100
-
30 h, enzyme purified from Pyrococcus furiosus and recombinant enzyme retains 80 to 90% of the initial activity Pyrococcus furiosus