Application | Comment | Organism |
---|---|---|
food industry | the enzyme suitable for hydrolysis of lactose at temperatures at 70-80°C | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galactose | measured with lactose as the substrate | Pyrococcus furiosus | |
D-glucose | measured with 2-nitrophenyl beta-D-galactoside as the substrate | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.3 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.5, 80°C | Pyrococcus furiosus | |
107 | - |
lactose | pH 5.5, 80°C | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q51723 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
lactose + H2O | - |
Pyrococcus furiosus | D-glucose + D-galactose | - |
? | |
additional information | the transfer of the galactosyl group from lactose to acceptors such as lactose or D-glucose rather than water is significant and depends on the initial lactose concentration as well as the time-dependent substrate/product ratio during batchwise lactose conversion | Pyrococcus furiosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CelB | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermostability is not affected by the presence of Mg2+ | Pyrococcus furiosus |
70 | - |
t1/2: 60 d | Pyrococcus furiosus |
80 | - |
t1/2: about 22 d | Pyrococcus furiosus |
90 | - |
t1/2: 3.2 d | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Pyrococcus furiosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
76 | - |
D-glucose | pH 5.5, 80°C | Pyrococcus furiosus | |
270 | - |
D-galactose | pH 5.5, 80°C | Pyrococcus furiosus |