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Literature summary for 3.2.1.B28 extracted from

  • Hansson, T.; Andersson, M.; Wehtje, E.; Adlercreutz, P.
    Influence of water activity on the competition between beta-glycosidase-catalysed transglycosylation and hydrolysis in aqueous hexanol (2001), Enzyme Microb. Technol., 29, 527-534.
No PubMed abstract available
Search for more literature for 3.2.1.B28

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
46
-
 pentyl beta-D-glucopyranoside pH 5.0, 80°C, 6% water, 94% hexanol Pyrococcus furiosus

Organic Solvent Stability

Organic Solvent Comment Organism
hexanol enzyme catalyzes both hydrolysis and transglycosylation of glycosidc substrates. In hexanol/water two-phase systems, hydrolysis is by far the dominating reaction even though the total activity increases. In hexanol containing various amounts of water, the selectivity for the alcohol increases with increasing water activity. This counteracts the effect of higher water concentration and the transglycosylation/hydrolysis ratio increases with increasing water activity Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q51723
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + beta-D-glucopyranose
-
 ?
additional information enzyme catalyzes both hydrolysis and transglycosylation of glycosidc substrates. In hexanol/water two-phase systems, hydrolysis is by far the dominating reaction even though the total activity increases. In hexanol containing various amounts of water, the selectivity for the alcohol increases with increasing water activity. This counteracts the effect of higher water concentration and the transglycosylation/hydrolysis ratio increases with increasing water activity Pyrococcus furiosus ?
-
 ?
pentyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus pentanol + beta-D-glucopyranose
-
 ?