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3.2.1.73: licheninase

This is an abbreviated version!
For detailed information about licheninase, go to the full flat file.

Word Map on EC 3.2.1.73

Reaction

beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranose
+ 4 H2O = 5 beta-D-glucopyranose

Synonyms

(1,3)(1,4)-beta-D-glucan-4-glucanohydrolase, (1->3,1->4)-beta-glucanase isoenzyme EII, 1,3-1,4-beta-D-glucan 4-glucanohydrolase, 1,3-1,4-beta-D-glucan glucanohydrolase, 1,3-1,4-beta-D-glucan-4-glucano hydrolase, 1,3-1,4-beta-D-glucanase, 1,3-1,4-beta-glucanase, 1,3;1,4-beta-glucan 4-glucanohydrolase, 1,3;1,4-beta-glucan endohydrolase, Af-EGL7, Afu6g01800, Beg1, beta-(1,3-1,4)-glucanase, beta-(1--> 3), (1--> 4)-D-glucan 4-glucanohydrolase, beta-1,3-1,4 glucanase, beta-1,3-1,4-D-glucanase, beta-1,3-1,4-glucanase, beta-1,3;1,4-glucanase, beta-1-3, 1-4 glucan 4-glucanohydrolase, beta-glucanase, BG1, Bg1314, Bga1, bgc, bgi, Bgl, bgl5-1, BglA, BglA13, BglA16, BglA51, BglBB, bglBC1, BGlc8H, BglM2, BglS, BglT, BglTO, Bglu16A, bifunctional xylanase/endoglucanase, BLB369, BLB369 endo-beta-1,3-1,4-glucanase, Blc8H, BP_Cel9A, Cel5F, CP7 beta-1,3-1,4-glucanase, CtGlu16A, Cthe_0211, CtLic16A, CtLic26A, E-LICHN, EG1, EGL, EII, endo-(1,3)(1,4)-beta-glucanase, endo-(1,3;1,4)-beta-glucanase, endo-beta-1,3-1,4 glucanase, endo-beta-1,3-1,4-glucanase, endo-beta-1,3;1,4-glucan-D-glycosyl hydrolase, endo-beta-glucanase, endoglucanase, endotype beta-1,3-1,4-glucanase, Fisuc_2961, Fsbeta-glucanase, FSU_0226, Gcs2, GH7 endo-1,4-beta-glucanase, GHF16 TFsbeta-glucanase, GHF17 barley 1,3-1,4-beta-D-glucanase, Glc16A, GLU-1, GLU-3, glu369, Gluc5_26A, GluIII, GluUS570, glycoside hydrolase family 9 endoglucanase, GyrA, H(A16-M), lam1, LamA, laminarinase, Lic16A, Lic8H, LicA, LicB, Lichenase, lichenase-2, LicKM, licM, LicMB, LicS, McLic1, mHG, Mixed linkage beta-glucanase, More, NFEg16A, PbBglu16A, PlicA, PtLic16A, Ra0505, RuCelA, TaGlu34, TC2, TC5, TF-glu, TF-glucanase, theme C glycoside hydrolase family 9 endo-beta-glucanase, TM1752, US8_01508, XynIII, XynZ, ZgLamA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.73 licheninase

Purification

Purification on EC 3.2.1.73 - licheninase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3.1fold to homogeneity by ammonium sulfate fractionation, dialysis and anion exchange chromatography, followed by hydrophobic interaction chromatography
affinity chromatography of epoxy-activated sepharose 6B and ultrafiltration technique
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by ammonium sulfate precipitation and two steps of ion-exchange chromatographies. Purified 122.5fold with an apparent homogeneity and a recovery yield of 8.9%
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by gel filtration and ultrafiltration
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DEAE Sephadex A50 column chromatography and Sephacryl S-200 gel filtration
Thermomonospora sp.
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DEAE-Sepharose CL-6B column chromatography
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GSTrap FF column chromatography and HiTrap Benzamidine FF column chromatography
Hi-Trap Q column chromatography and CHT-II column chromatography
immobilized metal ion affinity chromatography, gel filtration, anion exchange chromatography
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mutants purified on Ni-NTA affinity column, more than 95% purity
native enzyme 37fold from strain YC-9 by ammonium sulfate precipitation, anion exchange chromatography, and gel filtration, recombinant enzyme from Escherichia coli strain BL21 (DE3) cell culture supernatant by ammonium sulfate precipitation, anion exchange chromatography, and gel filtration
native enzyme 6.8fold by ammonium sulfate fractionation and two different steps of anion exchange chromatography, to homogeneity
native enzyme by 16fold ammonium sulfate fractionation, combined cation and anion exchange chromatography, and another anion exchange chromatography
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native extracellular enzyme 0.63fold from cell-free culture supernatant by heat treatment at 50°C for 10 min, anion exchange chromatography, lyophilization, and gel filtration to homogeneity
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native extracellular enzyme 14.24fold from culture supernatant by ultrafiltration and gel filtration to homogeneity
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native extracellular enzyme from cell culture supernatant 7.3fold to homogeneity by ammonium sulfate fractionation, and anion exchange and hydrophobic interaction chromatography
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native extracellular isozymes EG1 and EG2 0.24fold and 11.6fold, respectively, from cell-free culture supernatant by dialysis and concentration with PEG 400, followed by anion exchange chromatography and gel filtration, both isozymes to homogeneity
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Ni-IDA Sepharose resin column chromatography
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Ni-NTA agarose column chromatography
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Ni-NTA column chromatography
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Ni-NTA His Bind resin column chromatography
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on Ni-NTA affinity column
parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase
parental enzymes, hybrid beta-glucanase enzyme H1 which contains the 107 amino-terminal residues of mature Bacillus amyloliquefaciens beta-glucanase and the 107 carboxyl-terminal amino acid residues of Bacillus beta-glucanase and hybrid enzyme H2 which consists of the 105 amino-terminal residues from the Bacillus macerans enzyme and the carboxyl-terminal 107 amino acids from Bacillus amyloliquefaciens
Q-Sepharose FF column twice, Ni-NTA affinity column for the Escherichia coli protein, Q-Sepharose FF column for the Pichia pastoris protein
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recombinant enzyme
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recombinant enzyme from Escherichia coli
recombinant enzyme from Escherichia coli strain C43(DE3), the signal peptide is cleaved
KM079629
recombinant GST-tagged EGL1 from Escherichia coli by glutathione affinity chromatography, the tag is cleaved off by thrombin, followed by benzamidine resin chromatography
recombinant GST-tagged EGL2 from Escherichia coli by glutathione affinity chromatography, the tag is cleaved off by thrombin, followed by benzamidine resin chromatography
recombinant His-tagged enzyme 2.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by dialysis, ultrafiltration, and nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
recombinant His-tagged isolated catalytic domain of the enzyme from Escherichia coli strain BL21trxB(DE3) by nickel affinity chromatography and dialysis, tag cleavage by factor Xa digestion, and removal of the tag by another step of nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)
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recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, tag cleavage and again nickel affinity chromatography, recombinant extracellular wild-type and mutants V18Y, W203Y, and V18Y/W203Y enzymes from Pichia pastoris cell culture supernatant by dialysis and anion exchange chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant mutant chimeric laccase/beta-1,3-1,4-glucanase enzyme from Escherichia coli by nickel affinity chromatography and ultrafiltration
recombinant N-terminally His-tagged linear and circular enzymes from Escherichia coli strains BL21(DE3) or JM109(DE3) by nickel affinity chromatography
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recombinant protein
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secreted recombinant OmpA-His6-tagged enzyme from cell culture supernatant by nickel affinity chromatography
Sephadex G-100 gel filtration
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SP-Sepharose column chromatography and Sephacryl S-100 gel filtration
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the cloned protein from the recombinant strain BL21/pET21a-RuCelA is purified using Ni-NTA resin
wild type and mutant enzymes W101F, W101Y, E103D, E103Q, D105N, D105K, E107D, E107Q, and E107H
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