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Literature summary for 3.2.1.73 extracted from

  • Tang, Y.; Yang, S.; Yan, Q.; Zhou, P.; Cui, J.; Jiang, Z.
    Purification and characterization of a novel beta-1,3-1,4-glucanase (lichenase) from thermophilic Rhizomucor miehei with high specific activity and its gene sequence (2012), J. Agric. Food Chem., 60, 2354-2361.
    View publication on PubMed
Search for more literature for 3.2.1.73

Cloned(Commentary)

Cloned (Comment) Organism
gene RmLic16A, DNA and amino acid sequence determination and analysis, sequence comparisons Rhizomucor miehei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 2.0 mg/ml with barley beta-glucan and 1.4 mg/ml with lichenan at pH 5.5, 60°C Rhizomucor miehei

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Rhizomucor miehei
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31564
-
 1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation Rhizomucor miehei
33700
-
 gel filtration Rhizomucor miehei
35400
-
 1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation Rhizomucor miehei

Organism

Organism UniProt Comment Textmining
Rhizomucor miehei H9BZ70 gene RmLic16A
-
Rhizomucor miehei CAU432 H9BZ70 gene RmLic16A
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme contains two possible N-glycosylation sites at 72 (NKT) and 275 (NPT), respectively Rhizomucor miehei

Purification (Commentary)

Purification (Comment) Organism
native enzyme 6.8fold by ammonium sulfate fractionation and two different steps of anion exchange chromatography, to homogeneity Rhizomucor miehei

Source Tissue

Source Tissue Comment Organism Textmining
additional information high-level extracellular enzyme production of 6230 U/mL on oat flour at 3% w/v as a carbon source at 50 °C Rhizomucor miehei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28820 33480 purified native enzyme, substrate barley beta-glucan, pH 5.5, 60°C Rhizomucor miehei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O best substrate Rhizomucor miehei cellotriose + cellotetraose + cellobiose + D-glucose oligosaccharides, mainly trisaccharide and tetrasaccharide ?
barley beta-glucan + H2O best substrate Rhizomucor miehei CAU432 cellotriose + cellotetraose + cellobiose + D-glucose oligosaccharides, mainly trisaccharide and tetrasaccharide ?
lichenan + H2O 95.2% activity compared to barley beta-glucan Rhizomucor miehei cellotriose + ? oligosaccharides, mainly trisaccharide ?
lichenan + H2O 95.2% activity compared to barley beta-glucan Rhizomucor miehei CAU432 cellotriose + ? oligosaccharides, mainly trisaccharide ?
additional information thin layer chromatography product analysis, overview. Poor activity with laminarin, no activity with birchwood xylan, carboxymethyl cellulose, pullulan, soluble starch, locust bean gum, cellulose, Avicel, beta-1,3-glucan, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-galactopyranoside, substrate specificity, overview Rhizomucor miehei ?
-
 ?
additional information thin layer chromatography product analysis, overview. Poor activity with laminarin, no activity with birchwood xylan, carboxymethyl cellulose, pullulan, soluble starch, locust bean gum, cellulose, Avicel, beta-1,3-glucan, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-galactopyranoside, substrate specificity, overview Rhizomucor miehei CAU432 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation Rhizomucor miehei
More peptide mapping of native enzyme Rhizomucor miehei

Synonyms

Synonyms Comment Organism
beta-1,3-1,4-glucanase
-
 Rhizomucor miehei
endo-beta-1,3-1,4-glucanase
-
 Rhizomucor miehei
Lichenase
-
 Rhizomucor miehei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Rhizomucor miehei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 activity range, profile overview Rhizomucor miehei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified native enzyme, no loss in activity at pH 5.5 for 30 min, half-life is 225 min Rhizomucor miehei
70
-
 purified native enzyme, pH 5.5, half-life is 101 min Rhizomucor miehei
75
-
 purified native enzyme, pH 5.5, half-life is 64 min Rhizomucor miehei
80
-
 purified native enzyme, pH 5.5, half-life is 53 min, 80% activity remaining after 30 min Rhizomucor miehei
85
-
 purified native enzyme, pH 5.5, half-life is 27 min, inactivation after 120 min Rhizomucor miehei
90
-
 purified native enzyme, pH 5.5, 60% activity remaining after 30 min Rhizomucor miehei
100
-
 purified native enzyme, pH 5.5, 40% activity remaining after 30 min Rhizomucor miehei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Rhizomucor miehei

pH Range

pH Minimum pH Maximum Comment Organism
3.5 7.5 activity range, profile overview Rhizomucor miehei

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7.5 purified native enzyme, retains 90% of its activity at 60°C for 30 min Rhizomucor miehei

pI Value

Organism Comment pI Value Maximum pI Value
Rhizomucor miehei sequence calculation
-
 3.9

General Information

General Information Comment Organism
evolution the enzyme is a member of the glycosylhydrolase faamily GH16 Rhizomucor miehei