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Literature summary for 3.2.1.73 extracted from

  • Yaish, M.W.; Doxey, A.C.; McConkey, B.J.; Moffatt, B.A.; Griffith, M.
    Cold-active winter rye glucanases with ice-binding capacity (2006), Plant Physiol., 141, 1459-1472.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Secale cereale

Organism

Organism UniProt Comment Textmining
Secale cereale Q1EMA6 cultivar Musketeer
-

Purification (Commentary)

Purification (Comment) Organism
GSTrap FF column chromatography and HiTrap Benzamidine FF column chromatography Secale cereale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O
-
Secale cereale D-glucose + ?
-
?

Synonyms

Synonyms Comment Organism
beta-1,3-1,4-glucanase
-
Secale cereale
GLU-1
-
Secale cereale
GLU-3
-
Secale cereale

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-8 60
-
Secale cereale

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-4
-
GLU-3 retains 14%-35% activity at -4°C Secale cereale
64
-
GLU-3 is irreversibly denatured above 64°C Secale cereale