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0.197
-
concentrated crude extract, at 50°C, pH 5
1.01
recombinant enzyme at 37°C
1.57
-
pH 6.0, 40°C, mutant D58E
10800
-
expressed in Pichia pastoris X-33, sodium citrate buffer, pH 6.0, 50°C, 10 min
11.6
mutant E11L, 45°C, pH 5.0
1106
native enzyme, substrate beta-D-glucan, pH 6.0, 50°C
11940
substrate barley beta-glucan, pH 7.0, 70°C
12.6
-
after 55.53fold purification, at 50°C, pH 5
12502
-
purified native enzyme, pH 6.0, 65°C, substrate oat beta-glucan
1280
-
recombinant enzyme, using barley beta-glucan as a substrate, at 50°C and pH 6.0
1282
mutant C17R/Q87T/D152G/Y307H/V330A/N344D, pH 5.0, 40°C
1335
-
recombinant enzyme, using lichenan as a substrate, at 50°C and pH 6.0
13527
-
purified native enzyme, pH 6.0, 65°C, substrate barley beta-glucan
1388
-
pH 6.0, 40°C, wild type
15 - 19
-
pH 6.0, mutant W105H
15 - 25
mutant N139A, 45°C, pH not specified in the publication
15.6
-
isozyme EG1, pH 5.0, 50°C, substrate lichenan
15.9
azo blue barley beta-glucan, pH 6.0, 50°C
1572
-
pH 6.0, mutant W165H
161
substrate barley beta-glucan, pH 6.0, 55°C
1621
-
pH 6.0, mutant W105F
1755
-
pH 6.0, mutant W198F
1791
-
pH 6.0, mutant W165F
18.89
Thermomonospora sp.
-
enzyme from culture filtrate, using xylan as a substrate, at 50°C, pH 7.0
1906
-
recombinant enzyme, using lichenan as a substrate
192
native enzyme, substrate lichenan, pH 6.0, 70°C
1986
mutant E47I, 45°C, pH not specified in the publication
2.22
substrate barley beta-D-glucan, pH 5.5, 50°C
2.43
-
pH 6.0, 40°C, mutant D58N
2.57
-
pH 6.0, 40°C, mutant E60D
2.7
-
purified recombinant linear enzyme and wild-type enzyme, substrate barley-beta-glucan, pH and temperature not specified in the publication
23
purified enzyme without addition of agents and metal ions, with lichenan as the substrate, pH 10.0, 55°C
234
-
mutant enzyme D317A, at 50°C
2373
purified native enzyme, pH 6.0, 65°C
24
purified recombinant His-tagged enzyme, substrate barley beta-glucan, pH 6.0, 30°C
24.83
KM079629
substrate lichenan, pH 6.0, 50°C
240
-
mutant enzyme D220A, at 50°C
241
-
mutant enzyme E323A, at 50°C
245
-
mutant enzyme E242A, at 50°C
2479
-
recombinant enzyme after 8.74fold purification, using barley beta-glucan as a substrate
25
Thermomonospora sp.
-
enzyme from culture filtrate, using lichenan as a substrate, at 50°C, pH 7.0
258
-
mutant enzyme D106A, at 50°C
262
mutant F40I, 45°C, pH not specified in the publication
26530
purified recombinant enzyme, pH 6.0, 40°C, substrate barley beta-glucan
266
-
mutant enzyme D258A, at 50°C
271
-
mutant enzyme E262A, at 50°C
2717
-
recombinant wild-type enzyme, pH 6.5, 40°C, substrate AZO blue beta-glucan
273
-
mutant enzyme D100A, at 50°C
274
-
mutant enzyme D195A, at 50°C
2740
substrate lichenan, pH 5.0, 60°C
275
native enzyme, substrate beta-D-glucan, pH 6.0, 70°C
283.8
-
recombinant enzyme from crude extract, using barley beta-glucan as a substrate
28820 - 33480
purified native enzyme, substrate barley beta-glucan, pH 5.5, 60°C
2941
-
pH 6.0, mutant W186F
296
-
mutant enzyme D196A, at 50°C
308
mutant R137Q, 40°C, pH not specified in the publication
32100
purified recombinant enzyme, pH 6.0, 40°C, substrate barley beta-glucan
3370
-
recombinant optimally-modified enzyme, pH 6.5, 40°C, substrate AZO blue beta-glucan
3581
mutant K64A, 50°C, pH not specified in the publication
3671
mutant K64M, 50°C, pH not specified in the publication
3936
-
wild-type, pH 6.5, 40°C
39520
purified recombinant enzyme, pH 6.0, 40°C, substrate barley beta-glucan
40 - 45
-
mutant N31C/T187C/P102C/N125C, pH 6.5, 40°C
4040
substrtae beta-D-glucan, pH 5.0, 60°C
4094
-
mutant E46P/S43E/H205P/S40E, pH 6.5, 40°C
41.66
-
purified native enzyme, pH 5.5, 50°C, substrate lichenan
41210
purified recombinant enzyme, pH 6.0, 40°C, substrate barley beta-glucan
419
-
pH 6.0, mutant W141F
4274
mutant K20S/N31C/CS40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C
43 - 46
mutant N44L, 55°C, pH not specified in the publication
43.2
purified enzyme without addition of agents and metal ions, with oat beta-D-glucan as the substrate, pH 10.0, 55°C
431.8
-
substrate barley beta-glucan, pH 6.0, 55°C
480
-
pH 6.0, mutant W148F
5.75
-
pH 6.0, 40°C, mutant E56D
50.9
-
crude culture supernatant
502
-
pH 6.0, 40°C, mutant G63A
52.7
purified enzyme without addition of agents and metal ions, with barley beta-D-glucan as the substrate, pH 10.0, 55°C
5393
purified recombinant enzyme, pH 6.0, 65°C
54.3
carboxymethylcellulose as substrate, pH 5.0, 50°C
545
-
pH 6.0, 40°C, mutant M39F
5694
wild-type, expressed in Escherichia coli, pH 5.0, 50°C
58.6
hydroxyethylcellulose as substrate, pH 5.0, 50°C
598.6
Thermomonospora sp.
-
after 31.6fold purification, using xylan as a substrate, at 50°C, pH 7.0
60 - 61
mutant N44Q, 55°C, pH not specified in the publication
607
wild-type, pH 5.0, 40°C
6242
-
122.5fold purified enzyme
626
-
substrate lichenan, pH 6.0-7.0, 45°C, mutant TFsW203F
644
mutant Y42L, 45°C, pH not specified in the publication
6520
mutant V18Y, expressed in Escherichia coli, pH 5.0, 50°C
69
-
pH 6.0, mutant W141H
699
mutant R137M, 40°C, pH not specified in the publication
70.4
substrate lichenan, pH 6.0, 55°C
7055
purified recombinant His-tagged enzyme, substrate barley beta-glucan, pH 6.2, 55°C
724
mutant N197D/M322V, pH 5.0, 40°C
748
-
mutant enzyme D314A, at 50°C
7500
purified recombinant enzyme, substrate barley beta-glucan, pH 6.0, 40°C
754
-
wild type enzyme, at 50°C
767
-
isozyme EG2, pH 5.0, 50°C, substrate lichenan
77.7
purified enzyme with addition of Co2+, with barley beta-D-glucan as the substrate, pH 10.0, 55°C
7833
wild-type, 50°C, pH 5.0
792.2
Thermomonospora sp.
-
after 31.6fold purification, using lichenan as a substrate, at 50°C, pH 7.0
7980
-
expressed in Escherichia coli, sodium citrate buffer, pH 6.0, 50°C, 10 min
803
mutant L62G, 45°C, pH not specified in the publication
82.3
-
wild-type, pH 6.0, 50°C
8208
substrate lichenan, pH 7.0, 70°C
85.3
purified recombinant enzyme, pH 6.5, 60°C, substrate barley beta-glucan
851
native enzyme, substrate lichenan, pH 6.0, 50°C
916
-
pH 6.0, mutant W112F
9225
-
purified native enzyme, pH 6.0, 65°C, substrate lichenan
9263
mutant W203Y, expressed in Escherichia coli, pH 5.0, 50°C
9967
mutant V18Y/W203Y, expressed in Escherichia coli, pH 5.0, 50°C
10.4
-
hybrid enzyme H2 which consists of the 105 amino-terminal residues from the Bacillus macerans enzyme and the carboxyl-terminal 107 amino acids from Bacillus amyloliquefaciens
10.4
-
hybrid enzyme H2 which consists of the 105 amino-terminal residues from the Bacillus macerans enzyme and the carboxyl-terminal 107 amino acids from Bacillus amyloliquefaciens
1746
fusion protein encoding beta-1,3-1,4-glucanase both from Bacillus amyloliquefaciens and Clostridium thermocellum, substrate lichenan, pH 6.0, 70°C
1746
fusion protein encoding beta-1,3-1,4-glucanase both from Bacillus amyloliquefaciens and Clostridium thermocellum, substrate lichenan, pH 6.0, 70°C
2065
wild-type
2065
-
wild type, sodium citrate buffer, pH 6.0, 50°C, 10 min
2434
fusion protein encoding beta-1,3-1,4-glucanase both from Bacillus amyloliquefaciens and Clostridium thermocellum, substrate beta-D-glucan, pH 6.0, 70°C
2434
fusion protein encoding beta-1,3-1,4-glucanase both from Bacillus amyloliquefaciens and Clostridium thermocellum, substrate beta-D-glucan, pH 6.0, 70°C
2490
-
wild-type, pH 6.5, 40°C
2490
wild-type, pH 6.5, 40°C
29
-
pH 6.0, mutant W54F
29
-
purified native enzyme, substrate is Hordeum vulgare beta-1,3-1,4-glucan
3722
-
-
3722
-
hybrid beta-glucanase enzyme H1 which contains the 107 amino-terminal residues of mature Bacillus amyloliquefaciens beta-glucanase and the 107 carboxyl-terminal amino acid residues of Bacillus beta-glucanase
3722
-
hybrid beta-glucanase enzyme H1 which contains the 107 amino-terminal residues of mature Bacillus amyloliquefaciens beta-glucanase and the 107 carboxyl-terminal amino acid residues of Bacillus beta-glucanase
70
-
pH 6.0, mutant W203R
8726
-
pH 6.0, mutant W203F
additional information
-
2.2 U/ml, pH 5.0, 60°C, substrate barley beta-glucan
additional information
-
4504 U/gds
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
100.3 U/ml, substrate barley beta-glucan, pH 5.0, 60°C
additional information
135.6 U/ml, substrate barley beta-glucan, pH 7.0, 70°C
additional information
-
45.0 U/ml, pH 3.0, 50-60°C, substrate lichenan
additional information
-
1.5 U/ml, pH and temperature not specified in the publication, substrate barley beta-glucan
additional information
-
20.3 U/ml, pH 4.8, 80°C, substrate barley beta-glucan
additional information
-
20025 U/gds, pH 5.5, 60°C
additional information
20025 U/gds, pH 5.5, 60°C
additional information
-
6230 U/ml, pH and temperature not specified in the publication
additional information
6230 U/ml, pH and temperature not specified in the publication
additional information
-
8.5 U/ml, pH 4.0-5.0, 50-60°C, substrate barley beta-glucan
additional information
-
9023.62 U/gds, pH and temperature not specified in the publication