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Literature summary for 3.2.1.73 extracted from

  • Lee, J.M.; Moon, S.Y.; Kim, Y.R.; Kim, K.W.; Lee, B.J.; Kong, I.S.
    Improvement of thermostability and halostability of beta-1,3-1,4-glucanase by substituting hydrophobic residue for Lys(48) (2017), Int. J. Biol. Macromol., 94, 594-602.
    View publication on PubMed
Search for more literature for 3.2.1.73

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure. The enzyme is primarily composed of a beta-sheet, with two juxtaposed curved antiparallel beta-sheets consisting of seven strands. It forms a single compact globular domain, creating a deep cavity as a carbohydrate-binding channel for beta-glucan. The carbohydrate-binding region is a type B domain Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants Comment Organism
K48A 123% of wild-type activity, thermostability and halostability are enhanced. Catalytic efficiency of mutants is enhanced as a result of the increase in substrate affinity. The half-lives of the mutant increases up to about 7fold at 60-70°C Bacillus sp. (in: Bacteria)
K48L 137% of wild-type activity, thermostability and halostability are enhanced. Catalytic efficiency of mutants is enhanced as a result of the increase in substrate affinity. The half-lives of the mutant increases up to about 7fold at 60-70°C Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
NaCl 30%, halflife of wild-type 230 h, mutant K48A 694 h, mutant K48L 693 h, respectively Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Barley beta-glucan wild-type, 2.1 mg/ml, mutant K48A 1.6 mg/ml, mutant K48L 1.4 mg/ml, respectively, pH 6.0, 50°C Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) KCCM 90078
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
82.3
-
 wild-type, pH 6.0, 50°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Bacillus sp. (in: Bacteria) beta-glucan oligosaccharides
-
 ?
barley beta-glucan + H2O
-
 Bacillus sp. (in: Bacteria) KCCM 90078 beta-glucan oligosaccharides
-
 ?

Synonyms

Synonyms Comment Organism
Bg1314
-
 Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.5
-
 Barley beta-glucan wild-type, pH 6.0, 50°C Bacillus sp. (in: Bacteria)
7.5
-
 Barley beta-glucan mutant K48A, pH 6.0, 50°C Bacillus sp. (in: Bacteria)
7.5
-
 Barley beta-glucan mutant K48L, pH 6.0, 50°C Bacillus sp. (in: Bacteria)