Cloned (Comment) | Organism |
---|---|
hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli | Bacillus amyloliquefaciens |
hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli | Paenibacillus macerans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli | Bacillus amyloliquefaciens |
additional information | hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli | Paenibacillus macerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase | - |
Bacillus subtilis | - |
- |
- |
Paenibacillus macerans | - |
parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase | - |
Purification (Comment) | Organism |
---|---|
parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase | Bacillus amyloliquefaciens |
parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase | Paenibacillus macerans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3722 | - |
- |
Bacillus amyloliquefaciens |
3722 | - |
- |
Paenibacillus macerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucan + H2O | beta-D-glucan from barley | Bacillus subtilis | additional information | - |
? | |
beta-D-glucan + H2O | beta-D-glucan from barley | Bacillus amyloliquefaciens | additional information | - |
? | |
beta-D-glucan + H2O | beta-D-glucan from barley | Paenibacillus macerans | additional information | - |
? | |
lichenin + H2O | - |
Bacillus subtilis | additional information | - |
? | |
lichenin + H2O | - |
Bacillus amyloliquefaciens | additional information | - |
? | |
lichenin + H2O | - |
Paenibacillus macerans | additional information | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Bacillus subtilis |
55 | - |
- |
Bacillus amyloliquefaciens |
65 | - |
- |
Paenibacillus macerans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 65 | more than 80% of maximal activity at 37°C and at 65°C | Bacillus subtilis |
37 | 65 | more than 80% of maximal activity at 37°C and at 65°C | Bacillus amyloliquefaciens |
55 | 75 | more than 80% of maximal activity at 55°C and at 75°C | Paenibacillus macerans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
pH 6.0, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase exhibit 90% of the initial activity, the parental enzymes retain 5% or less of the initial activity | Bacillus amyloliquefaciens |
70 | - |
pH 6.0, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase exhibit 90% of the initial activity, the parental enzymes retain 5% or less of the initial activity | Paenibacillus macerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Bacillus subtilis |
6.5 | - |
- |
Bacillus amyloliquefaciens |
7.6 | - |
- |
Bacillus subtilis |
7.6 | - |
- |
Bacillus amyloliquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | more than 80% of maximal activity at pH 5.5 and pH 7.0 | Bacillus amyloliquefaciens |
5.9 | 6.5 | more than 80% of maximal activity at pH 5.9 and pH 6.5 | Bacillus subtilis |
5.9 | 8.5 | more than 80% of maximal activity at pH 5.9 and pH 8.5 | Paenibacillus macerans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.1 | - |
65°C, 1 h, 95% loss of activity | Bacillus amyloliquefaciens |
4.1 | - |
65°C, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, 85%-95% of the initial activity is retained | Bacillus amyloliquefaciens |
4.1 | - |
65°C, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, 85%-95% of the initial activity is retained | Paenibacillus macerans |
4.1 | - |
65°C, 1 h, complete inactivation | Paenibacillus macerans |