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Literature summary for 3.2.1.73 extracted from

  • Olsen, O.; Borrisss, R.; Simon, O.; Thomsen, K.K.
    Hybrid Bacillus (1-3,1-4)-beta-glucanases: engineering thermostable enzymes by construction of hybrid genes (1991), Mol. Gen. Genet., 225, 177-185.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli Bacillus amyloliquefaciens
hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli Paenibacillus macerans

Protein Variants

Protein Variants Comment Organism
additional information hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli Bacillus amyloliquefaciens
additional information hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli Paenibacillus macerans

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase
-
Bacillus subtilis
-
-
-
Paenibacillus macerans
-
parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase
-

Purification (Commentary)

Purification (Comment) Organism
parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase Bacillus amyloliquefaciens
parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase Paenibacillus macerans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3722
-
-
Bacillus amyloliquefaciens
3722
-
-
Paenibacillus macerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucan + H2O beta-D-glucan from barley Bacillus subtilis additional information
-
?
beta-D-glucan + H2O beta-D-glucan from barley Bacillus amyloliquefaciens additional information
-
?
beta-D-glucan + H2O beta-D-glucan from barley Paenibacillus macerans additional information
-
?
lichenin + H2O
-
Bacillus subtilis additional information
-
?
lichenin + H2O
-
Bacillus amyloliquefaciens additional information
-
?
lichenin + H2O
-
Paenibacillus macerans additional information
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Bacillus subtilis
55
-
-
Bacillus amyloliquefaciens
65
-
-
Paenibacillus macerans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 65 more than 80% of maximal activity at 37°C and at 65°C Bacillus subtilis
37 65 more than 80% of maximal activity at 37°C and at 65°C Bacillus amyloliquefaciens
55 75 more than 80% of maximal activity at 55°C and at 75°C Paenibacillus macerans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
pH 6.0, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase exhibit 90% of the initial activity, the parental enzymes retain 5% or less of the initial activity Bacillus amyloliquefaciens
70
-
pH 6.0, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase exhibit 90% of the initial activity, the parental enzymes retain 5% or less of the initial activity Paenibacillus macerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Bacillus subtilis
6.5
-
-
Bacillus amyloliquefaciens
7.6
-
-
Bacillus subtilis
7.6
-
-
Bacillus amyloliquefaciens

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 more than 80% of maximal activity at pH 5.5 and pH 7.0 Bacillus amyloliquefaciens
5.9 6.5 more than 80% of maximal activity at pH 5.9 and pH 6.5 Bacillus subtilis
5.9 8.5 more than 80% of maximal activity at pH 5.9 and pH 8.5 Paenibacillus macerans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.1
-
65°C, 1 h, 95% loss of activity Bacillus amyloliquefaciens
4.1
-
65°C, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, 85%-95% of the initial activity is retained Bacillus amyloliquefaciens
4.1
-
65°C, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, 85%-95% of the initial activity is retained Paenibacillus macerans
4.1
-
65°C, 1 h, complete inactivation Paenibacillus macerans