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-4
GLU-3 retains 14%-35% activity at -4°C
20 - 40
-
the remaining activity decreased to 20% at 20-40°C and is completely destroyed at 50-70°C
20 - 50
-
30 min with about 50% of activity remaining at 50°C
22
-
11 days, complete inactivation of wild-type, 70% residual activity of mutant D70V
25 - 35
-
decrease in stability from 25°C to 35°C
25 - 40
purified recombinant enzyme, pH 6.0, 70 min, completely stable
30
12 h, 50% activity remaining
30 - 70
80% activity remaining
30 - 90
-
temperature effects on mutant W203F and on W203F mutant bifunctional hybrid enzymes, the latter is more resistant to heat treatment than the parental TFsW203F, overview
40 - 50
-
purified isozymes EG1 and EG2, retaining over 90% activity after 1 h
44.5
-
15 min, 50% of activity, mutant G44R
46
-
15 min, 50% of activity, mutant K23R
46.1
-
15 min, 50% of activity, mutant A79P
46.2
-
15 min, 50% of activity, mutant F85Y
47.5
-
15 min, 50% of activity, wild type
47.9
-
15 min, 50% of activity, mutant M298K, mutant T17D
48.2
-
15 min, 50% of activity, mutant N290H
50 - 60
-
sharp decrease in activity up to 60°C, but very stable below 50°C
50.8
-
wild-type, melting temperature
51.2
-
15 min, 50% of activity, mutant H300P
52
-
10 min, 50% inactivation, wild-type
54.9
-
mutant N31C/T187C/P102C/N125C, melting temperature
57
Tm of wild-type enzyme
62
-
melting temperature, wild-type
64
GLU-3 is irreversibly denatured above 64°C
66
-
10 min, 50% inactivation, mutant D70V
70 - 80
purified enzyme, pH 10.0, 30 min, loss of 80% activity
76.5
-
melting temperature, mutant E46P/S43E/H205P/S40E
80 - 90
purified recombinant enzyme, 70% and 40% activity remaining after 2 h at 80°C and 90°C, respectively, half-lives of the enzyme are 3.4 h and 1.4 h at 80°C and 90°C, respectively
100
purified native enzyme, pH 5.5, 40% activity remaining after 30 min
100
-
even when subjected to 100°C for 3 h, beta-glucanase activity does not show significant reduction
30 - 60
completely stable up to 50°C for 1 h and loss of 10% activity after 48 h at 50°C, 50% activity remaing at 55°C after 30 min and inactivation after 5 h at 55°C, rapid inactivation at 60°C
30 - 60
-
99.1, 87.8, 56.6, and 34.2% residual activity after 30 min preincubation at 30, 40, 50, and 60°C, respectively
30 - 60
-
104.5, 101.2, 67.9, and 21.2% residual activity after 30 min preincubation at 30, 40, 50, and 60°C, respectively
30 - 60
-
102, 91.2, 60.7, and 4.3% residual activity after 30 min preincubation at 30, 40, 50, and 60°C, respectively
30 - 60
-
98, 74.3, 2.4, and 0% residual activity after 30 min preincubation at 30, 40, 50, and 60°C, respectively
40
-
pH 5.5, 10 min, stable
40
-
recombinant enzyme, pH 6.0, 24 h, stable
40
-
purified native enzyme, 75% activity remaining after 120 min
40 - 55
24 h, completely stable
40 - 55
-
the enzyme remains stable between 40 and 55°C after 30 min incubation
45
the wild-type maintains more than 85% of the original activity for 10 min
45
purified enzyme, pH 10.0, 30 min, completely stable up to
45
-
recombinant enzyme, pH 6.0, 24 h, 28% loss of activity
50
-
purified enzyme, pH 5.0, half-life is 150 min
50
purified recombinant His-tagged enzyme, pH 6.0, 30 min, completely stable
50
-
recombinant enzyme, pH 6.0, 24 h, 41% loss of activity
50
-
10 min, about 25% loss of activity
50
30 min, more than 90% of maximum activity
50
purified recombinant His-tagged enzyme retaining more than 70% activity at up to 50°C for 2 h
50
-
purified native enzyme, 60% activity remaining after 120 min
50 - 70
-
after incubation at 50, 60, and 70°C for 30 min, the residual activity is 95, 60, and 0%, respectively
50 - 70
-
after 2 h incubation at 50°C and 60°C, the residual activity remaines 100% and 50%, respectively, while the enzymatic activity is abolished after 3 min incubation at 70°C
50 - 70
-
the purified native enzyme shows half-lives of 209, 130 and 69 min at 50, 60 and 70°C, respectively
55
purified enzyme, pH 10.0, 30 min, loss of 50% activity
55
-
recombinant enzyme, pH 6.0, 24 h, 70% loss of activity
55
3 h, 85% activity remaining
55
purified recombinant His-tagged enzyme, half-life is about 50 min, 10% activity remaining after 2 h
60
-
purified enzyme, pH 5.0, half-life is 135 min
60
purified recombinant enzyme, retains more than 90% activity after 2 h
60
2 h, more than 85% residual activity, both wild-type and mutant K142N/Q203L/N214D
60
-
2 h, 90% activity remaining
60
1 h, 50% activity remaining
60
-
purified isozymes EG1 and EG2, half-lives are 55 min and 5 h, respectively
60
-
10 min, about 35% loss of activity
60
-
pH 5.0-6.0, 10 min, stable. pH 6, in presence of 10 mM CaCl2 and serum albumin, stable for 120 min
60
half-life of wild-type 46.2 min, of mutant K48A 346 min, of mutant K48L 138 min, respectively
60
-
15 min, pH 6.5, stable
60
-
the melting temperature is 50.8°C. The half-life of the enzyme at 60°C is 59 min. The enzyme retains more than 60% of its activity at up to 75°C and is inactivated at 80°C after 10 min of incubation
60
stable for at least 1 h
60
-
10 min, 50% loss of activity, crude enzyme extract
60
-
pH 5.5, 10 min, complete loss of activity
60
-
4 min, 50% inactivation, wild-type, 11 min, 50% inactivation, mutant D70V
60
-
10 min, complete inactivation
60
purified recombinant His-tagged enzyme, inactivation within 10 min, in presence of 5 mM CaCl2 the enzyme retains over 80% activity after 2 h
60
after 30 min and 1 h at 60°C, the enzyme retains about 60% and 35% of activity, respectively
60
-
purified native enzyme, half-life is 80 min
60
purified native enzyme, no loss in activity at pH 5.5 for 30 min, half-life is 225 min
60 - 70
-
purified recombinant enzyme, the wild-type enzymes activity decline rapidly above 60°C, while the optimally-modified enzyme is more stable and shows activity up to 70°C
60 - 70
-
half-lives are 80 min at 60°C and 21 min at 70°C
65
-
purified enzyme, pH 5.0, half-life is 90 min
65
-
hybrid beta-glucanase enzyme H1 which contains the 107 amino-terminal residues of mature Bacillus amyloliquefaciens beta-glucanase is stable for more than 1 h at pH 5.5. The 107 carboxyl-terminal amino acid residues of Bacillus beta-glucanase. Hybrid enzyme H2 which consists of the 105 amino-terminal residues from the Bacillus macerans enzyme and the carboxyl-terminal 107 amino acids from Bacillus amyloliquefaciens irreversible thermoinactivated within 20-25 min
65
-
the linear enzyme form loses 50% activity within 3 min, whereas the two circular variants have 6fold (LicA-C1) and 16fold (LicA-C2) increased half-life time of inactivation
65
purified recombinant His-tagged enzyme, pH 6.0, 30 min, loss of 50% activity
65
-
purified native enzyme, half-life is over 68 days
65
-
hybrid beta-glucanase enzyme H1 which contains the 107 amino-terminal residues of mature Bacillus amyloliquefaciens beta-glucanase is stable for more than 1 h at pH 5.5. The 107 carboxyl-terminal amino acid residues of Bacillus beta-glucanase. Hybrid enzyme H2 which consists of the 105 amino-terminal residues from the Bacillus macerans enzyme and the carboxyl-terminal 107 amino acids from Bacillus amyloliquefaciens irreversible thermoinactivated within 20-25 min
65
purified recombinant His-tagged enzyme, in presence of 5 mM CaCl2 the enzyme retains 40% activity after 10 min, and 20% after 20 min, inactivation after 1 h
70
-
purified recombinant enzyme, pH 7.0, 40% activity remaining after 6 h
70
-
purified enzyme, pH 5.0, half-life is 30 min
70
purified recombinant enzyme, retains more 75% activity after 10 min
70
-
pH 6.0, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase exhibit 90% of the initial activity, the parental enzymes retain 5% or less of the initial activity
70
-
purified isozymes EG1 and EG2, half-lives are 10 min and 52 min, respectively
70
2 h, 70% activity remaining
70
purified recombinant His-tagged enzyme, pH 6.0, 30 min, inactivation
70
1 h, 80% activity remaining
70
half-life of wild-type 4.5 min, of mutant K48A 30.5 min, of mutant K48L 15.1 min, respectively
70
-
1 h, 60% residual activity, mutant E46P/S43E/H205P/S40E
70
4 h, 60% activity remaining
70
5 min, 48% residual activity, 60 min, 30% residual activity
70
-
1 h, 15% loss of activity
70
-
purified native enzyme, half-life is 125 h
70
30 min, 65% of maximum activity
70
-
pH 6.0, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase exhibit 90% of the initial activity, the parental enzymes retain 5% or less of the initial activity
70
-
purified native enzyme, half-life is 21 min
70
purified native enzyme, pH 5.5, half-life is 101 min
70
-
purified native enzyme, 30 min, over 80% activity remaining
70
purified recombinant enzyme, completely stable for 8 h, and loss of 20% activity after 22 h
75
-
purified recombinant wild-type enzyme, 10 min, 51% remaining activity in absence of Ca2+, 86% in presence of 1 mM Ca2+
75
-
1 h, 25% loss of activity
75
1 h, 75% activity remaining
75
-
purified native enzyme, half-life is 22 h
75
purified native enzyme, pH 5.5, half-life is 64 min
80
-
purified recombinant wild-type enzyme, 5 min, 54% remaining activity in absence of Ca2+, 73% in presence of 1 mM Ca2+
80
20 min, 80% residual activity
80
-
purified recombinant enzyme, pH 7.0, no activity remaining after 1 h
80
4 h, 80% activity remaining
80
purified recombinant enzyme, retains more 44% activity after 10 min
80
30 min, 50% activity remaining
80
purified recombinant enzyme, retains 54% activity at 80°C after incubation for 60 min
80
4 min, complete loss of activtiy for wild-type, 10 min, 60% residual activity for mutant T113S
80
1 h, 54% activity remaining
80
10 min, 60% activity remaining
80
-
purified native enzyme, half-life is 1 h
80
purified native enzyme, pH 5.5, half-life is 53 min, 80% activity remaining after 30 min
80
16 h, completely stable
80
Thermomonospora sp.
-
half-life of 5 min at 80°C
80
-
optimum condition for the beta-glucanase stability
85
5 min, 25% residual activity
85
-
purified native enzyme, half-life is about 5 min
85
purified native enzyme, pH 5.5, half-life is 27 min, inactivation after 120 min
90
10 min, 80% activity remaining
90
-
the residual activity of mHG at 90°C for 10 min is 83.45% of its maximum activity
90
10 min, 40% residual activity for mutant T113S
90
-
80% activity after 10 min
90
-
purified native enzyme, half-life is 80 s
90
purified native enzyme, pH 5.5, 60% activity remaining after 30 min
additional information
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Ca2+ stabilizes the enzyme at higher temperatures, thermal stability of recombinant wild-type enzyme and mutants in presence and absence of Ca2+, overview
additional information
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particularly thermostable in presence of Ca2+
additional information
-
Ca2+ stabilizes hybrid enzymes and parental enzymes against thermal inactivation
additional information
-
addition of 1 mM EDTA causes the linear enzyme to denature at 59.8°C, which is lower compared to the melting temperature of 62.6°C in the presence of calcium. At 1 mM EDTA, the circular enzyme variant LicA-C1 shows a melting temperature of 62.8°C, at 1 mM CaCl2 the melting temperature is 66.4°C
additional information
-
particularly thermostable in presence of Ca2+
additional information
-
particularly thermostable in presence of Ca2+
additional information
-
particularly thermostable in presence of Ca2+
additional information
-
particularly thermostable in presence of Ca2+
additional information
-
thermal inactivation follow first-order kinetics, overview
additional information
-
particularly thermostable in presence of Ca2+
additional information
-
particularly thermostable in presence of Ca2+
additional information
-
Ca2+ stabilizes hybrid enzymes and parental enzymes against thermal inactivation
additional information
-
particularly thermostable in presence of Ca2+