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Literature summary for 3.2.1.73 extracted from

  • Yang, S.; Xiong, H.; Yan, Q.; Yang, H.; Jiang, Z.
    Purification and characterization of a novel alkaline beta-1,3-1,4-glucanase (lichenase) from thermophilic fungus Malbranchea cinnamomea (2014), J. Ind. Microbiol. Biotechnol., 41, 1487-1495.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slightly activating Malbranchea cinnamomea
SDS slightly activating Malbranchea cinnamomea

Application

Application Comment Organism
additional information the alkaline beta-1,3-1,4-glucanase may have potential in industrial applications, such as detergent, paper and pulp industries Malbranchea cinnamomea

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Malbranchea cinnamomea
Cr3+
-
Malbranchea cinnamomea
Fe2+
-
Malbranchea cinnamomea
Fe3+
-
Malbranchea cinnamomea
Hg2+
-
Malbranchea cinnamomea
Mg2+
-
Malbranchea cinnamomea
Mn2+
-
Malbranchea cinnamomea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 0.69 mg/ml with barley beta-glucan, 1.11 mg/ml with oat beta-glucan, and 0.63 with lichenan, pH 10.0, 55°C Malbranchea cinnamomea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Malbranchea cinnamomea
Co2+ best activating metal ion Malbranchea cinnamomea
Cu2+ activates Malbranchea cinnamomea
K+ activates slightly Malbranchea cinnamomea
additional information the enzyme activity is not or poorly affected by EDTA, Tween-80, Triton X-100, H2O2, NaOCl, and diverse washing powders and soaps, overview Malbranchea cinnamomea
Ni2+ activates Malbranchea cinnamomea
Zn2+ activates Malbranchea cinnamomea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44700
-
1 * 44700, SDS-PAGE Malbranchea cinnamomea
46900
-
gel filtration Malbranchea cinnamomea

Organism

Organism UniProt Comment Textmining
Malbranchea cinnamomea S6DXG7 i.e. Malbranchea sulfurea
-
Malbranchea cinnamomea S168 S6DXG7 i.e. Malbranchea sulfurea
-

Purification (Commentary)

Purification (Comment) Organism
3.1fold to homogeneity by ammonium sulfate fractionation, dialysis and anion exchange chromatography, followed by hydrophobic interaction chromatography Malbranchea cinnamomea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23
-
purified enzyme without addition of agents and metal ions, with lichenan as the substrate, pH 10.0, 55°C Malbranchea cinnamomea
43.2
-
purified enzyme without addition of agents and metal ions, with oat beta-D-glucan as the substrate, pH 10.0, 55°C Malbranchea cinnamomea
52.7
-
purified enzyme without addition of agents and metal ions, with barley beta-D-glucan as the substrate, pH 10.0, 55°C Malbranchea cinnamomea
77.7
-
purified enzyme with addition of Co2+, with barley beta-D-glucan as the substrate, pH 10.0, 55°C Malbranchea cinnamomea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
Malbranchea cinnamomea ?
-
?
barley beta-glucan + H2O
-
Malbranchea cinnamomea S168 ?
-
?
lichenan + H2O
-
Malbranchea cinnamomea ?
-
?
lichenan + H2O
-
Malbranchea cinnamomea S168 ?
-
?
additional information the enzyme shows strict substrate specificity for barley beta-glucan, oat beta-glucan and lichenan, but is not active with other tested polysaccharides and synthetic 4-nitrophenyl derivates Malbranchea cinnamomea ?
-
?
additional information the enzyme shows strict substrate specificity for barley beta-glucan, oat beta-glucan and lichenan, but is not active with other tested polysaccharides and synthetic 4-nitrophenyl derivates Malbranchea cinnamomea S168 ?
-
?
oat beta-glucan + H2O
-
Malbranchea cinnamomea ?
-
?
oat beta-glucan + H2O
-
Malbranchea cinnamomea S168 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 44700, SDS-PAGE Malbranchea cinnamomea

Synonyms

Synonyms Comment Organism
beta-1,3-1,4-glucanase
-
Malbranchea cinnamomea
Lichenase
-
Malbranchea cinnamomea
McLic1
-
Malbranchea cinnamomea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Malbranchea cinnamomea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 temperature profile overview Malbranchea cinnamomea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
purified enzyme, pH 10.0, 30 min, completely stable up to Malbranchea cinnamomea
55
-
purified enzyme, pH 10.0, 30 min, loss of 50% activity Malbranchea cinnamomea
70 80 purified enzyme, pH 10.0, 30 min, loss of 80% activity Malbranchea cinnamomea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
lichenan pH 10.0, 55°C Malbranchea cinnamomea
0.016
-
Barley beta-glucan pH 10.0, 55°C Malbranchea cinnamomea
0.022
-
Oat beta-glucan pH 10.0, 55°C Malbranchea cinnamomea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Malbranchea cinnamomea

pH Range

pH Minimum pH Maximum Comment Organism
4 11 pH profile overview Malbranchea cinnamomea

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 2 purified enzyme, 37°C, 30 min, inactivation Malbranchea cinnamomea
4 10 purified enzyme, stable at Malbranchea cinnamomea
11
-
purified enzyme, 37°C, 30 min, loss of 50% activity Malbranchea cinnamomea