Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slightly activating | Malbranchea cinnamomea | |
SDS | slightly activating | Malbranchea cinnamomea |
Application | Comment | Organism |
---|---|---|
additional information | the alkaline beta-1,3-1,4-glucanase may have potential in industrial applications, such as detergent, paper and pulp industries | Malbranchea cinnamomea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Malbranchea cinnamomea | |
Cr3+ | - |
Malbranchea cinnamomea | |
Fe2+ | - |
Malbranchea cinnamomea | |
Fe3+ | - |
Malbranchea cinnamomea | |
Hg2+ | - |
Malbranchea cinnamomea | |
Mg2+ | - |
Malbranchea cinnamomea | |
Mn2+ | - |
Malbranchea cinnamomea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.69 mg/ml with barley beta-glucan, 1.11 mg/ml with oat beta-glucan, and 0.63 with lichenan, pH 10.0, 55°C | Malbranchea cinnamomea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Malbranchea cinnamomea | |
Co2+ | best activating metal ion | Malbranchea cinnamomea | |
Cu2+ | activates | Malbranchea cinnamomea | |
K+ | activates slightly | Malbranchea cinnamomea | |
additional information | the enzyme activity is not or poorly affected by EDTA, Tween-80, Triton X-100, H2O2, NaOCl, and diverse washing powders and soaps, overview | Malbranchea cinnamomea | |
Ni2+ | activates | Malbranchea cinnamomea | |
Zn2+ | activates | Malbranchea cinnamomea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44700 | - |
1 * 44700, SDS-PAGE | Malbranchea cinnamomea |
46900 | - |
gel filtration | Malbranchea cinnamomea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malbranchea cinnamomea | S6DXG7 | i.e. Malbranchea sulfurea | - |
Malbranchea cinnamomea S168 | S6DXG7 | i.e. Malbranchea sulfurea | - |
Purification (Comment) | Organism |
---|---|
3.1fold to homogeneity by ammonium sulfate fractionation, dialysis and anion exchange chromatography, followed by hydrophobic interaction chromatography | Malbranchea cinnamomea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23 | - |
purified enzyme without addition of agents and metal ions, with lichenan as the substrate, pH 10.0, 55°C | Malbranchea cinnamomea |
43.2 | - |
purified enzyme without addition of agents and metal ions, with oat beta-D-glucan as the substrate, pH 10.0, 55°C | Malbranchea cinnamomea |
52.7 | - |
purified enzyme without addition of agents and metal ions, with barley beta-D-glucan as the substrate, pH 10.0, 55°C | Malbranchea cinnamomea |
77.7 | - |
purified enzyme with addition of Co2+, with barley beta-D-glucan as the substrate, pH 10.0, 55°C | Malbranchea cinnamomea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Malbranchea cinnamomea | ? | - |
? | |
barley beta-glucan + H2O | - |
Malbranchea cinnamomea S168 | ? | - |
? | |
lichenan + H2O | - |
Malbranchea cinnamomea | ? | - |
? | |
lichenan + H2O | - |
Malbranchea cinnamomea S168 | ? | - |
? | |
additional information | the enzyme shows strict substrate specificity for barley beta-glucan, oat beta-glucan and lichenan, but is not active with other tested polysaccharides and synthetic 4-nitrophenyl derivates | Malbranchea cinnamomea | ? | - |
? | |
additional information | the enzyme shows strict substrate specificity for barley beta-glucan, oat beta-glucan and lichenan, but is not active with other tested polysaccharides and synthetic 4-nitrophenyl derivates | Malbranchea cinnamomea S168 | ? | - |
? | |
oat beta-glucan + H2O | - |
Malbranchea cinnamomea | ? | - |
? | |
oat beta-glucan + H2O | - |
Malbranchea cinnamomea S168 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44700, SDS-PAGE | Malbranchea cinnamomea |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Malbranchea cinnamomea |
Lichenase | - |
Malbranchea cinnamomea |
McLic1 | - |
Malbranchea cinnamomea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Malbranchea cinnamomea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | temperature profile overview | Malbranchea cinnamomea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
purified enzyme, pH 10.0, 30 min, completely stable up to | Malbranchea cinnamomea |
55 | - |
purified enzyme, pH 10.0, 30 min, loss of 50% activity | Malbranchea cinnamomea |
70 | 80 | purified enzyme, pH 10.0, 30 min, loss of 80% activity | Malbranchea cinnamomea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
lichenan | pH 10.0, 55°C | Malbranchea cinnamomea | |
0.016 | - |
Barley beta-glucan | pH 10.0, 55°C | Malbranchea cinnamomea | |
0.022 | - |
Oat beta-glucan | pH 10.0, 55°C | Malbranchea cinnamomea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Malbranchea cinnamomea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | pH profile overview | Malbranchea cinnamomea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 2 | purified enzyme, 37°C, 30 min, inactivation | Malbranchea cinnamomea |
4 | 10 | purified enzyme, stable at | Malbranchea cinnamomea |
11 | - |
purified enzyme, 37°C, 30 min, loss of 50% activity | Malbranchea cinnamomea |