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3.2.1.73: licheninase

This is an abbreviated version!
For detailed information about licheninase, go to the full flat file.

Word Map on EC 3.2.1.73

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beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranose
+ 4 H2O = 5 beta-D-glucopyranose

Synonyms

(1,3)(1,4)-beta-D-glucan-4-glucanohydrolase, (1->3,1->4)-beta-glucanase isoenzyme EII, 1,3-1,4-beta-D-glucan 4-glucanohydrolase, 1,3-1,4-beta-D-glucan glucanohydrolase, 1,3-1,4-beta-D-glucan-4-glucano hydrolase, 1,3-1,4-beta-D-glucanase, 1,3-1,4-beta-glucanase, 1,3;1,4-beta-glucan 4-glucanohydrolase, 1,3;1,4-beta-glucan endohydrolase, Af-EGL7, Afu6g01800, Beg1, beta-(1,3-1,4)-glucanase, beta-(1--> 3), (1--> 4)-D-glucan 4-glucanohydrolase, beta-1,3-1,4 glucanase, beta-1,3-1,4-D-glucanase, beta-1,3-1,4-glucanase, beta-1,3;1,4-glucanase, beta-1-3, 1-4 glucan 4-glucanohydrolase, beta-glucanase, BG1, Bg1314, Bga1, bgc, bgi, Bgl, bgl5-1, BglA, BglA13, BglA16, BglA51, BglBB, bglBC1, BGlc8H, BglM2, BglS, BglT, BglTO, Bglu16A, bifunctional xylanase/endoglucanase, BLB369, BLB369 endo-beta-1,3-1,4-glucanase, Blc8H, BP_Cel9A, Cel5F, CP7 beta-1,3-1,4-glucanase, CtGlu16A, Cthe_0211, CtLic16A, CtLic26A, E-LICHN, EG1, EGL, EII, endo-(1,3)(1,4)-beta-glucanase, endo-(1,3;1,4)-beta-glucanase, endo-beta-1,3-1,4 glucanase, endo-beta-1,3-1,4-glucanase, endo-beta-1,3;1,4-glucan-D-glycosyl hydrolase, endo-beta-glucanase, endoglucanase, endotype beta-1,3-1,4-glucanase, Fisuc_2961, Fsbeta-glucanase, FSU_0226, Gcs2, GH7 endo-1,4-beta-glucanase, GHF16 TFsbeta-glucanase, GHF17 barley 1,3-1,4-beta-D-glucanase, Glc16A, GLU-1, GLU-3, glu369, Gluc5_26A, GluIII, GluUS570, glycoside hydrolase family 9 endoglucanase, GyrA, H(A16-M), lam1, LamA, laminarinase, Lic16A, Lic8H, LicA, LicB, Lichenase, lichenase-2, LicKM, licM, LicMB, LicS, McLic1, mHG, Mixed linkage beta-glucanase, More, NFEg16A, PbBglu16A, PlicA, PtLic16A, Ra0505, RuCelA, TaGlu34, TC2, TC5, TF-glu, TF-glucanase, theme C glycoside hydrolase family 9 endo-beta-glucanase, TM1752, US8_01508, XynIII, XynZ, ZgLamA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.73 licheninase

Cloned

Cloned on EC 3.2.1.73 - licheninase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C-terminally truncated (10 kDa), overexpression in Escherichia coli BL21 (DE3) and Pichia pastoris X-33, mutants with increased catalytic efficiency and thermotolerance
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DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic analysis. Recombinant expression of the enzyme fused to the Trx-tag/His-tag/S-tag sequences in Escherichia coli strain BL21 (DE3), the enzyme is located in the cell lysate and the cell culture supernatant
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21/pET21a
expressed in Escherichia coli strain BL21 (DE3)
-
expressed in Lactobacillus reuteri strain Pg4
expressed in Nicotiana benthamina as fusion protein
-
expressed in Pichia pastoris strain GS115
-
expression as GST-fusion protein
-
expression in Escherichia coli
expression in Escherichia coli and Pichia pastoris
expression in Escherichia coli, isolation and characterization of the gene
-
expression in Pichia pastoris
expression in Pichia pastoris and Escherichia coli, recombinant enzmye expressed in Pichia pastoris shows higher specific activity
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), heterologous expression of extracellular wild-type and mutant enzymes in Pichia pastoris
expression of the His-tagged isolated catalytic domain of the enzyme, residues 31-251, in Escherichia coli strain BL21trxB(DE3) with an extra linker AGAGA at its N-terminus
expression of the N-terminally His-tagged enzyme with or without inteins, constructs for linear and circular enzymes, in Escherichia coli strains BL21(DE3) or JM109(DE3). The inteins are spliced out
-
expression of wild-type enzyme and mutant circular enzyme constructs in Escherichia coli strain XL1-Blue
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gene AFUA_6G01800 or Af-egl7, DNA and amino acid sequnce determination and analysis, sequence comparisons, quantitative RT-PCR exoression analysis, recombinant expression in Escherichia coli strain Rosetta (DE3) pLysS in inclusion bodies, subcloning in Escherichia coli strain DH10beta
gene beg1, recombinant expression in Saccharomyces cerevisiae, the enzyme is secreted
gene bg1, recombinant expression in Escherichia coli and in Pichia pastoris
gene bg1314, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21
gene bga1, recombinant expression in Escherichia coli, the enzyme is secreted
gene bgc, recombinant expression in Bacillus subtilis and in Bacillus megaterium
gene bgi, recombinant expression in Escherichia coli, the enzyme is secreted
gene bgl, recombinant expression in Escherichia coli, the enzyme is secreted
gene bgl5-1, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109
gene bgl5-1, recombinant expression in Escherichia coli, the enzyme is secreted
gene bglA, recombinant expression in Escherichia coli
gene bglA13, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli
gene bglA16, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli
gene bglA51, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli
gene bglBB, recombinant expression in Escherichia coli, the enzyme is secreted
gene bglM2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli
gene bglS, expression of mutant chimeric laccase/beta-1,3-1,4-glucanase enzyme in Escherichia coli
gene bglS, recombinant expression in Escherichia coli
gene bglS, recombinant expression in Escherichia coli, in Saccharomyces cerevisiae, in plants, and in mammalian cells, the enzyme is secreted
gene bglS, recombinant expression in Saccharomyces cerevisiae
gene bp_cel9A, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21
gene FSU_0226, recombinant expression in Escherichia coli and in Pichia pastoris
gene gcs2, recombinant expression in Escherichia coli, the enzyme is secreted
gene glu369, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the extracellular enzyme in Escherichia coli TOP10 cells using vector pKJD4 also encoding the OmpA signal sequence for Escherichia coli periplasmic localization, periplasmic secretion of the recombinant OmpA-His6-tagged enzyme
gene lic16A, recombinant expression in Escherichia coli
gene LicB, expression of mature enzyme and isolated catalytic domain, as well as of the enzyme truncation mutants in Escherichia coli strain BL21
-
gene licB, recombinant expression in Escherichia coli and in Pichia pastoris
gene mt-lic, genomic library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree and sequence comparisons, recombinant expression in Escherichia coli strain C43(DE3)
KM079629
gene NFEg16A, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) as intracellular soluble protein
gene OsEGL1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, functional expression od GST-tagged EGL1 in Escherichia coli
gene OsEGL2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, functional expression of GST-tagged EGL2 in Escherichia coli
gene plicA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene PtLic16A, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene RmLic16A, DNA and amino acid sequence determination and analysis, sequence comparisons
gene SU40-glu, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene US8_01508, recombinant expression in Escherichia coli, the enzyme is secreted
-
gene Zobellia_2431, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
hybrid beta-glucanase enzyme H1 which contains the 107 amino-terminal residues of mature Bacillus amyloliquefaciens beta-glucanase and the 107 carboxyl-terminal amino acid residues of Bacillus beta-glucanase and hybrid enzyme H2 which consists of the 105 amino-terminal residues from the Bacillus macerans enzyme and the carboxyl-terminal 107 amino acids from Bacillus amyloliquefaciens, expression in Escherichia coli
hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli
hybrid from Bacillus macerans and Bacillus amyloliquefaciens
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hybrid from Bacillus macerans and Bacillus amyloliquefaciens, H(A16-M)
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into pET3a vector and expressed in Escherichia coli strain BL21(DE3)pLysS
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overexpression in Escherichia coli XL1-blue and BL21 with His-tag
-
plasmid carrying the truncated 1,3-1,4-beta-D-glucanase gene in the pET26b(+) vector, transformed into Escherichia coli XL1-Blue. Overexpression of the truncated form and mutants in Escherichia coli BL21 (DE3) cells
recombinant expression and secretion from Bacillus subtilis strain WB800 using vector pP43JM2
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recombinant expression in Escherichia coli
recombinant expression in Escherichia coli and in Pichia pastoris
recombinant expression in Escherichia coli, and in Mus musculus, and in Sus scrofa parotid gland
recombinant expression in transgenic Nicotiana tabacum plants
-
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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recombinant expression, the enzyme is secreted
transgenic barley expressing a protein-engineered, thermostable (1,3-1,4)-beta-glucanase during germination
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wild-type and mutants cloned into vector pET26b(+) and expressed in Escherichia coli BL21 (DE3)