Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | activates to 139.72% | Bacillus tequilensis | |
Triton X-100 | activates to 221.30% | Bacillus tequilensis | |
Tween-20 | activates to 159.20% | Bacillus tequilensis | |
Tween-80 | activates to 267.73% | Bacillus tequilensis |
Application | Comment | Organism |
---|---|---|
food industry | the thermostable enzyme can be useful in mashing at 72°C of brewing processes | Bacillus tequilensis |
Cloned (Comment) | Organism |
---|---|
gene bgl5-1, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 | Bacillus tequilensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Bacillus tequilensis | |
Ca2+ | 16% inhibition at 10 mM, 15% at 1 mM | Bacillus tequilensis | |
Co2+ | 6% inhibition at 10 mM, 16% at 1 mM | Bacillus tequilensis | |
DTT | - |
Bacillus tequilensis | |
EDTA | - |
Bacillus tequilensis | |
Fe2+ | 92% inhibition at 10 mM | Bacillus tequilensis | |
Li+ | 48.5% inhibition at 10 mM, 18% at 1 mM | Bacillus tequilensis | |
Mg2+ | activates by 89% at 1 mM, inhibits 50% at 10 mM | Bacillus tequilensis | |
Mn2+ | 45% inhibition at 10 mM, 20% at 1 mM | Bacillus tequilensis | |
Zn2+ | 52% inhibition at 10 mM, 44% at 1 mM | Bacillus tequilensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 10.0 mg/ml with barley beta-glucan, pH 6.0, 40°C, recombinant enzyme | Bacillus tequilensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates by 89% at 1 mM, inhibits 50% at 10 mM | Bacillus tequilensis | |
additional information | metal ions are required for enzyme activity and also disulfide linkage may be involved in catalytic activity | Bacillus tequilensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 26900, about, sequence calculation, x * 24000, recombinant enzyme, SDS-PAGE | Bacillus tequilensis |
26900 | - |
x * 26900, about, sequence calculation, x * 24000, recombinant enzyme, SDS-PAGE | Bacillus tequilensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | Bacillus tequilensis | - |
? | - |
? | |
barley beta-glucan + H2O | Bacillus tequilensis CGX5-1 | - |
? | - |
? | |
lichenan + H2O | Bacillus tequilensis | - |
? | - |
? | |
lichenan + H2O | Bacillus tequilensis CGX5-1 | - |
? | - |
? | |
oat beta-glucan + H2O | Bacillus tequilensis | - |
? | - |
? | |
oat beta-glucan + H2O | Bacillus tequilensis CGX5-1 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus tequilensis | K0A689 | isolated from Asian giant hornet, Vespa mandarinia, nest, gene bgl5-1 | - |
Bacillus tequilensis CGX5-1 | K0A689 | isolated from Asian giant hornet, Vespa mandarinia, nest, gene bgl5-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by dialysis, ultrafiltration, and nickel affinity chromatography | Bacillus tequilensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7500 | - |
purified recombinant enzyme, substrate barley beta-glucan, pH 6.0, 40°C | Bacillus tequilensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Bacillus tequilensis | ? | - |
? | |
barley beta-glucan + H2O | best substrate | Bacillus tequilensis | ? | - |
? | |
barley beta-glucan + H2O | - |
Bacillus tequilensis CGX5-1 | ? | - |
? | |
barley beta-glucan + H2O | best substrate | Bacillus tequilensis CGX5-1 | ? | - |
? | |
lichenan + H2O | - |
Bacillus tequilensis | ? | - |
? | |
lichenan + H2O | - |
Bacillus tequilensis CGX5-1 | ? | - |
? | |
additional information | no activity with carboxymethyl cellulose, xylan, laminarin, and soluble starch, substrate specificity, overview | Bacillus tequilensis | ? | - |
? | |
additional information | no activity with carboxymethyl cellulose, xylan, laminarin, and soluble starch, substrate specificity, overview | Bacillus tequilensis CGX5-1 | ? | - |
? | |
oat beta-glucan + H2O | - |
Bacillus tequilensis | ? | - |
? | |
oat beta-glucan + H2O | - |
Bacillus tequilensis CGX5-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26900, about, sequence calculation, x * 24000, recombinant enzyme, SDS-PAGE | Bacillus tequilensis |
Synonyms | Comment | Organism |
---|---|---|
1,3-1,4-beta-glucanase | - |
Bacillus tequilensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Bacillus tequilensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | high activity within this range, recombinant enzyme | Bacillus tequilensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | purified recombinant enzyme, pH 6.0, 70 min, completely stable | Bacillus tequilensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus tequilensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | activity range, over 80% activity within the range of pH 5.0-7.5, recombinant enzyme | Bacillus tequilensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus tequilensis | sequence calculation | - |
6.76 |