Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Paecilomyces sp. (in: Eurotiomycetes) | |
additional information | the J18 strain produces the maximum level of extracellular beta-glucanase (135.6 U/ml) when grown in a medium containing corncob (5%, w/v) at 50°C for 4 days | Paecilomyces sp. (in: Eurotiomycetes) | |
N-bromosuccinimide | - |
Paecilomyces sp. (in: Eurotiomycetes) |
Application | Comment | Organism |
---|---|---|
industry | properties make the enzyme highly suitable for industrial applications | Paecilomyces sp. (in: Eurotiomycetes) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | inhibits the enzyme activity at 4 mM by 56.9% | Paecilomyces sp. (in: Eurotiomycetes) | |
beta-mercaptoethanol | inhibits the enzyme activity at 4 mM by 82.7% | Paecilomyces sp. (in: Eurotiomycetes) | |
Ca2+ | inhibits the enzyme activity at 4 mM by 55.5% | Paecilomyces sp. (in: Eurotiomycetes) | |
Cu2+ | completely inhibits the enzyme activity at 4 mM | Paecilomyces sp. (in: Eurotiomycetes) | |
DTT | inhibits the enzyme activity at 4 mM by 82.3% | Paecilomyces sp. (in: Eurotiomycetes) | |
EDTA | inhibits the enzyme activity at 4 mM by 43.4% | Paecilomyces sp. (in: Eurotiomycetes) | |
Fe2+ | inhibits the enzyme activity at 4 mM by 14.9% | Paecilomyces sp. (in: Eurotiomycetes) | |
Fe3+ | inhibits the enzyme activity at 4 mM by 13.5% | Paecilomyces sp. (in: Eurotiomycetes) | |
Hg2+ | completely inhibits the enzyme activity at 4 mM | Paecilomyces sp. (in: Eurotiomycetes) | |
Mg2+ | inhibits the enzyme activity at 4 mM by 52.9% | Paecilomyces sp. (in: Eurotiomycetes) | |
Mn2+ | completely inhibits the enzyme activity at 4 mM | Paecilomyces sp. (in: Eurotiomycetes) | |
Na+ | inhibits the enzyme activity at 4 mM by 47.3%; inhibits the enzyme activity at 4 mM by 80.3% | Paecilomyces sp. (in: Eurotiomycetes) | |
Ni2+ | inhibits the enzyme activity at 4 mM by 11.5% | Paecilomyces sp. (in: Eurotiomycetes) | |
SDS | inhibits the enzyme activity at 4 mM by 88.2% | Paecilomyces sp. (in: Eurotiomycetes) | |
Sr2+ | inhibits the enzyme activity at 4 mM by 49% | Paecilomyces sp. (in: Eurotiomycetes) | |
Zn2+ | inhibits the enzyme activity at 4 mM by 11.6% | Paecilomyces sp. (in: Eurotiomycetes) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31690 | - |
mass spectrometry | Paecilomyces sp. (in: Eurotiomycetes) |
34600 | - |
gel filtration | Paecilomyces sp. (in: Eurotiomycetes) |
38600 | - |
1 * 38600, SDS-PAGE | Paecilomyces sp. (in: Eurotiomycetes) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paecilomyces sp. (in: Eurotiomycetes) | - |
- |
- |
Paecilomyces sp. (in: Eurotiomycetes) J18 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate precipitation and two steps of ion-exchange chromatographies. Purified 122.5fold with an apparent homogeneity and a recovery yield of 8.9% | Paecilomyces sp. (in: Eurotiomycetes) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50.9 | - |
crude culture supernatant | Paecilomyces sp. (in: Eurotiomycetes) |
6242 | - |
122.5fold purified enzyme | Paecilomyces sp. (in: Eurotiomycetes) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) | beta-glucan oligosaccharides | - |
? | |
barley beta-glucan + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) J18 | beta-glucan oligosaccharides | - |
? | |
lichenan + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) | lichenan oligosaccharides | - |
? | |
lichenan + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) J18 | lichenan oligosaccharides | - |
? | |
additional information | beta-glucanase does not act toward avicel, carboxymethylcellulose, cellulose, filter paper, beta-1,3-glucan, hydroxyethyl-cellulose, laminarin, starch, pullulan, birchwood xylan, locust bean gum, cellobiose, and xylobiose. No activity for p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-fucopyranoside, p-nitrophenyl-beta-D-galactopyranoside, and p-nitrophenyl-beta-D-mannopyranoside | Paecilomyces sp. (in: Eurotiomycetes) | ? | - |
? | |
additional information | beta-glucanase does not act toward avicel, carboxymethylcellulose, cellulose, filter paper, beta-1,3-glucan, hydroxyethyl-cellulose, laminarin, starch, pullulan, birchwood xylan, locust bean gum, cellobiose, and xylobiose. No activity for p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-fucopyranoside, p-nitrophenyl-beta-D-galactopyranoside, and p-nitrophenyl-beta-D-mannopyranoside | Paecilomyces sp. (in: Eurotiomycetes) J18 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38600, SDS-PAGE | Paecilomyces sp. (in: Eurotiomycetes) |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Paecilomyces sp. (in: Eurotiomycetes) |
beta-glucanase | - |
Paecilomyces sp. (in: Eurotiomycetes) |
endo-beta-1,3-1,4-glucanase | - |
Paecilomyces sp. (in: Eurotiomycetes) |
Lichenase | - |
Paecilomyces sp. (in: Eurotiomycetes) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Paecilomyces sp. (in: Eurotiomycetes) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | - |
Paecilomyces sp. (in: Eurotiomycetes) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
for 30 min | Paecilomyces sp. (in: Eurotiomycetes) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
81.6 | - |
Barley beta-glucan | calculated as D-glucose units released from beta-glucan | Paecilomyces sp. (in: Eurotiomycetes) | |
166.4 | - |
lichenan | calculated as D-glucose units released from lichenan | Paecilomyces sp. (in: Eurotiomycetes) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Paecilomyces sp. (in: Eurotiomycetes) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Paecilomyces sp. (in: Eurotiomycetes) |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Paecilomyces sp. (in: Eurotiomycetes) | isoelectric focusing | - |
4.2 |