Cloned (Comment) | Organism |
---|---|
expression of the His-tagged isolated catalytic domain of the enzyme, residues 31-251, in Escherichia coli strain BL21trxB(DE3) with an extra linker AGAGA at its N-terminus | Acetivibrio thermocellus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant catalytic domain, sitting drop vapour diffusion method, mixing of 0.002 ml of 30 mg/ml protein in 25 mM Tris pH 7.5, 150 mM NaCl, and 0.002 ml reservori solution containing 0.04 M citric acid, 0.06 M Bis-Tris propane, pH 6.4, 18% w/v PEG 3350, 25°C, 7 days, optimized method, X-ray diffraction structure determination and analysis at 1.95 A resolution, molecular replacement method | Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, about, recombinant catalytic domain, SDS-PAGE | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Acetivibrio thermocellus | the enzyme catalyzes the specific hydrolysis of internal beta-1,4-glycosidic bonds adjacent to the 3-O-substituted glucose residues in mixed-linked beta-glucans. The Clostridium thermocellum enzyme exhibits a high specific activity towards barley beta-glucan and lichenan, but is not active towards laminarin, curdlan and cellulosic substrates | ? | - |
? | |
additional information | Acetivibrio thermocellus DSM 1237 | the enzyme catalyzes the specific hydrolysis of internal beta-1,4-glycosidic bonds adjacent to the 3-O-substituted glucose residues in mixed-linked beta-glucans. The Clostridium thermocellum enzyme exhibits a high specific activity towards barley beta-glucan and lichenan, but is not active towards laminarin, curdlan and cellulosic substrates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A3DBX3 | - |
- |
Acetivibrio thermocellus DSM 1237 | A3DBX3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isolated catalytic domain of the enzyme from Escherichia coli strain BL21trxB(DE3) by nickel affinity chromatography and dialysis, tag cleavage by factor Xa digestion, and removal of the tag by another step of nickel affinity chromatography | Acetivibrio thermocellus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranose + 4 H2O = 5 beta-D-glucopyranose | beta-1,3-1,4-glucanase catalyzes strict endo-hydrolysis of the beta-1,4-glycosidic linkage adjacent to a 3-O-substituted glucose residue in the mixed-linked beta-glucans via a double-displacement mechanism | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
barley beta-glucan + H2O | - |
Acetivibrio thermocellus DSM 1237 | ? | - |
? | |
lichenan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
lichenan + H2O | - |
Acetivibrio thermocellus DSM 1237 | ? | - |
? | |
additional information | the enzyme catalyzes the specific hydrolysis of internal beta-1,4-glycosidic bonds adjacent to the 3-O-substituted glucose residues in mixed-linked beta-glucans. The Clostridium thermocellum enzyme exhibits a high specific activity towards barley beta-glucan and lichenan, but is not active towards laminarin, curdlan and cellulosic substrates | Acetivibrio thermocellus | ? | - |
? | |
additional information | the enzyme catalyzes the specific hydrolysis of internal beta-1,4-glycosidic bonds adjacent to the 3-O-substituted glucose residues in mixed-linked beta-glucans. The Clostridium thermocellum enzyme exhibits a high specific activity towards barley beta-glucan and lichenan, but is not active towards laminarin, curdlan and cellulosic substrates | Acetivibrio thermocellus DSM 1237 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, about, recombinant catalytic domain, SDS-PAGE | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Acetivibrio thermocellus |
CtGlu16A | - |
Acetivibrio thermocellus |
Lichenase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Acetivibrio thermocellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 12 | 50% of maximal activity within this range | Acetivibrio thermocellus |
General Information | Comment | Organism |
---|---|---|
evolution | beta-1,3-1,4-glucanases from various microorganisms and plants belong to glycoside hydrolase families 16 and 17, respectively | Acetivibrio thermocellus |