Cloned (Comment) | Organism |
---|---|
gene PtLic16A, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Paecilomyces sp. 'thermophila' |
Protein Variants | Comment | Organism |
---|---|---|
C263S | random mutagenesis, the mutant shows an acidic shift in the pH optimum and altered substrate specificity compared to the wild-type enzyme | Paecilomyces sp. 'thermophila' |
D221G2 | random mutagenesis, the mutant shows unaltered properties compared to the wild-type enzyme | Paecilomyces sp. 'thermophila' |
D56G | random mutagenesis, the mutant shows an acidic shift in the pH optimum and altered substrate specificity compared to the wild-type enzyme | Paecilomyces sp. 'thermophila' |
D56G/D221G/C263S | random mutagenesis, mutant PtLic16AM2 shows an acidic shift in the pH optimum and altered substrate specificity compared to the wild-type enzyme. Mutation D221G alone does not lead to altered enzyme properties | Paecilomyces sp. 'thermophila' |
additional information | screening of the random mutant library, mutations D56G, D221G, and C263S have only minor effects on specific activity and pH stability | Paecilomyces sp. 'thermophila' |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 3.69 mg/ml for the wild-type enzyme and 3.30 mg/ml for mutant D56G/D221G/C263S, with barley beta-glucan at pH 5.0, 70°C | Paecilomyces sp. 'thermophila' |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Paecilomyces sp. 'thermophila' | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Paecilomyces sp. 'thermophila' | beta-1,3-1,4-glucanase strictly cleaves the beta-1,4-D-glucosidic bonds adjacent to beta-1,3-linkages in mixed linked beta-glucans | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paecilomyces sp. 'thermophila' | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) | Paecilomyces sp. 'thermophila' |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Paecilomyces sp. 'thermophila' | ? | - |
? | |
additional information | beta-1,3-1,4-glucanase strictly cleaves the beta-1,4-D-glucosidic bonds adjacent to beta-1,3-linkages in mixed linked beta-glucans | Paecilomyces sp. 'thermophila' | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular structure modeling and analysis | Paecilomyces sp. 'thermophila' |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Paecilomyces sp. 'thermophila' |
PtLic16A | - |
Paecilomyces sp. 'thermophila' |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Paecilomyces sp. 'thermophila' |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | activity range, wild-type and mutant enzymes, profile overview | Paecilomyces sp. 'thermophila' |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
102 | - |
Barley beta-glucan | pH 5.0, 70°C, wild-type enzyme | Paecilomyces sp. 'thermophila' | |
143 | - |
Barley beta-glucan | pH 5.0, 70°C, mutant D56G/D221G/C263S | Paecilomyces sp. 'thermophila' |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid residues at positions 56 and 263 are important in determining optimal pH activity | Paecilomyces sp. 'thermophila' |
5 | - |
mutant D56G/D221G/C263S | Paecilomyces sp. 'thermophila' |
5.5 | - |
mutant C263S | Paecilomyces sp. 'thermophila' |
6 | - |
mutant D56G | Paecilomyces sp. 'thermophila' |
7 | - |
wild-type enzyme and mutant D221G | Paecilomyces sp. 'thermophila' |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | activity range, wild-type and mutant enzymes, profile overview | Paecilomyces sp. 'thermophila' |
General Information | Comment | Organism |
---|---|---|
additional information | molecular structure modeling and analysis | Paecilomyces sp. 'thermophila' |